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Method of preparing sugar by utilizing sweet potato residues

A technology of sweet potato residue and sweet potato starch wastewater, applied in the biological field, can solve the problems of incomplete degradation, refractory degradation, low hydrolysis rate, etc., and achieve the effect of improving conversion rate, low cost, and strong specificity

Active Publication Date: 2013-04-17
SHANDONG BIO SUNKEEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The dry basis of sweet potato dregs generally contains more than 50% starch and 22-26% fiber. The main components of fiber are cellulose, hemicellulose, lignin and pectin, etc., which are carbohydrates with a dense structure of glucose sugar groups Compound, which is difficult to be degraded by the cellulase secreted by commercially available Trichoderma konii; the sweet potato is separated by rasp mill and starch produced by sieving equipment, and the residual starch in the potato residue is liquefied by α-amylase and saccharified by glucoamylase , its degradation is not complete; other polymer polysaccharides are more difficult to degrade, and the hydrolysis rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Sweet potato dregs (moisture content 13.85%, starch content 52.09%) produced by a company in Shanxi, crushed to 60 mesh, weighed 5.0kg, in a 30L enzymolysis tank, added 20kg of sweet potato starch waste water to adjust the slurry (solid-liquid ratio 1:4) , adjust the pH6.5 with 10% NaOH, add high temperature resistant α-amylase (Thermostable α-amylase) by adding 20U of enzyme per gram of feed, the slurry is heated up to 95°C, incubated for 45 minutes, and used I 2 The liquefaction test turns red and the liquefaction is complete. Cool the slurry to 58-60°C, adjust the pH to 4.8 with 10% HCl, add glucoamylase (Glucoamylase) 100U per gram of material, add 100U of cellulase per gram of material and acid protease (Acid) protease) Add enzyme 10U per gram of material, (keep warm for about 40h), stir, the concentration of reducing sugar in the slurry will no longer increase, stop saccharification, extinguish the enzyme at 110°C, cool down to 50°C, and use a centrifuge to separa...

Embodiment 2

[0042] Sweet potato dregs (moisture 13.85%, starch content 52.09%) produced by a company in Shanxi, crushed to 60 mesh, weighed 5.0kg, in a 30L enzymolysis tank, added 22.5kg of sweet potato starch waste water to adjust slurry (solid-liquid ratio 1: 4.5 ), adjust the pH to 5.5 with sodium bicarbonate, add thermostable α-amylase (Thermostable α-amylase) and add 20U of enzyme per gram of feed, the slurry is heated up to 95°C, incubated for 45 minutes, and used I 2 The liquefaction test turns red and the liquefaction is complete. Cool the slurry to 58-60°C, adjust the pH to 3.5 with acetic acid 1, add glucoamylase (Glucoamylase) 250U per gram of material, cellulase (cellulase) 400U per gram of material and acid protease (Acid protease) ) Add 15U of enzyme per gram of material, (keep warm for about 40 hours), stir until the concentration of reducing sugar in the slurry no longer rises, stop saccharification, inactivate the enzyme at 110°C, cool to 65°C, and use a centrifuge for so...

Embodiment 3

[0044] Sweet potato dregs (moisture content 13.85%, starch content 52.09%) produced by a company in Shanxi, crushed to 60 mesh, weighed 5.0kg, in a 30L enzymolysis tank, added 25kg of sweet potato starch waste water to adjust the slurry (solid-liquid ratio 1:5) , adjust the pH to 8.0 with 10% NaOH, add high temperature resistant α-amylase (Thermostable α-amylase) and add 15U of enzyme per gram of feed, the slurry is heated up to 95°C, incubated for 45 minutes, and used I 2 The liquefaction test turns red and the liquefaction is complete. Cool the slurry to 58-60°C, adjust the pH to 5.5 with 10% HCl, add 300U of enzyme per gram of Glucoamylase, 500U of enzyme per gram of cellulase and acid protease at 60°C protease) Add enzyme 12U per gram of material, keep warm for about 40 hours, stir, the concentration of reducing sugar in the slurry will no longer increase, stop saccharification, inactivate the enzyme at 110°C, cool down to 40°C, and use a centrifuge to separate the solid f...

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Abstract

The invention relates to filed of biology and in particular relates to a method of preparing sugar by utilizing biomass sweet potato residues, used for microbial fermentation, as a sugar source. The method comprises the following steps of: adjusting the pH of sweet potato residue powder or wet residue to be 5.5-8.0, and carrying out heat preservation by adding alpha-amylase; adjusting the pH to be 3.0-5.5, carrying out enzyme removal after carrying out heat preservation at 40-65 DEG C by adding saccharifying enzyme, acid protease and cellulose; and carrying out solid-liquid separation on a feed liquid, and concentrating the liquid to obtain a sugar liquid product, wherein the ingredient of the sugar liquid product is basically the glucose. The preparation method is simple in process, highin specificity, high in product quality, and high in yield, can be used for solving the problem that the environment is severely polluted by the sweet potato residues, and is good in industrial application prospect.

Description

technical field [0001] The invention relates to the field of biology, in particular to a sugar production method using biomass sweet potato residue for microbial fermentation as a sugar source. Background technique [0002] Sweet potato is one of the important food crops in my country. According to statistics, the planting and processing of sweet potato in my country ranks first in the world, with a total output of 85.2 million tons, of which 55% (about 46.86 million tons) is converted into industrial raw materials. Sweet potato slag is a large amount of by-products produced during the processing of fresh sweet potato starch or accumulated as waste, accounting for about 10% of the raw material. The huge biomass renewable resources have not been effectively utilized and seriously polluted the environment. [0003] Fresh sweet potatoes generally contain 30% dry matter and 70% moisture. Sweet potato processing produces a large amount of wastewater containing cell fluid, which ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/20C12P19/14C12P19/02
Inventor 吴允山严共鸿易勇寇正恩
Owner SHANDONG BIO SUNKEEN
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