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HIP/PAP recombinant adenovirus and application thereof in resisting ulcerative colitis

A recombinant adenovirus, adenovirus technology, applied in the application, drug combination, biochemical equipment and methods, etc., can solve problems such as poor effect and limited use of patients

Inactive Publication Date: 2012-07-11
THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
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Problems solved by technology

At present, the clinical treatment of UC is mainly based on aminosalicylic acid and hormone preparations, but the effect on some UC, especially severe patients, is poor.
In recent years, clinical application of biological agents including anti-tumor necrosis factor, α 4 beta 7 Integrin, CD 25 Monoclonal antibodies such as monoclonal antibodies are effective in treating UC, but they are only effective for some patients. At the same time, the side effects after application also limit the use of patients. Therefore, it is urgent to develop safe and effective new UC treatment drugs and methods.

Method used

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  • HIP/PAP recombinant adenovirus and application thereof in resisting ulcerative colitis
  • HIP/PAP recombinant adenovirus and application thereof in resisting ulcerative colitis
  • HIP/PAP recombinant adenovirus and application thereof in resisting ulcerative colitis

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Embodiment Construction

[0033] The present invention provides a new application and new method for treating ulcerative colitis by expressing HIP / PAP molecules in the colonic mucosa, successfully constructing a recombinant adenovirus Ad-HIP / PAP expressing HIP / PAP, and enema through recombinant adenovirus Highly express HIP / PAP in rat colonic mucosa. In vivo and in vitro test results show that HIP / PAP can relieve bloody stool and diarrhea in ulcerative colitis, significantly reduce colonic mucosal inflammation, significantly down-regulate the expression of pro-inflammatory factors, reduce colonic mucosal oxidative damage, promote colonic mucosal repair, and accelerate ulcer healing. The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0034] 1. Amplification of target gene HIP / PAP

[0035] Get the colonic mucosal tissue of patients with ulcerative colitis, extract total RNA b...

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Abstract

The invention discloses an HIP / PAP recombinant adenovirus and application of the recombinant virus in resisting ulcerative colitis. A recombinant adenovirus Ad-HIP / PAP expressing HIP / PAP is constructed, and the HIP / PAP is efficiently expressed in colon mucosa of a rat through enema of the recombinant adenovirus. After expression of HIP / PAP is up-regulated, reduction in body weight of rats with ulcerative colitis is not obvious, and conditions of hematochezia and diarrhea are obviously relieved compared with those of a control group. After sacrificing the rats, obvious alleviation of inflammation of colon mucosa is observed, the expression of proinflammatory cytokines is significantly down-regulated, and oxidation damage of the colon mucosa is obviously reduced. In addition, the HIP / PAP also can promote the proliferation of epithelial cells of the colon mucosa, thereby promoting the repair of the colon mucosa, accelerating ulcer healing and having significant therapeutic effect on the ulcerative colitis.

Description

technical field [0001] The invention belongs to the technical field of treating ulcerative colitis, and relates to HIP / PAP recombinant adenovirus and its anti-ulcerative colitis application. Background technique [0002] Ulcerative colitis (UC) is an unexplained disease with mucosal necrosis and inflammation as the main pathological manifestations. Its incidence is increasing year by year in China, and there are more and more severe patients. At present, the clinical treatment of UC is mainly based on aminosalicylic acid and hormone preparations, but the effect on some UC, especially severe patients, is poor. In recent years, clinical application of biological agents including anti-tumor necrosis factor, α 4 beta 7 Integrin, CD 25 Monoclonal antibodies such as monoclonal antibodies are only effective for some patients, and the side effects after application also limit the use of patients. Therefore, it is urgent to develop safe and effective new UC treatment drugs and met...

Claims

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Application Information

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IPC IPC(8): C12N15/861A61K48/00A61P1/04
Inventor 郝志明吕颐菲李爽杨小飞苏厚强蒋会平田筱青殷燕
Owner THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
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