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Method for extracting pulmonary mesenchymal stem cells

A technology of mesenchymal stem cells and keratinocytes, applied in the biological field, can solve the problems of the small number of stem cells and the inconvenience of experimental research, and achieve the effect of high cell purity, good vitality, and abundant cell sources

Inactive Publication Date: 2012-07-11
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

The results of animal experiments show that the number of stem cells that can be obtained by simple bronchoalveolar lavage is very small, which has caused great inconvenience to experimental research

Method used

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  • Method for extracting pulmonary mesenchymal stem cells
  • Method for extracting pulmonary mesenchymal stem cells
  • Method for extracting pulmonary mesenchymal stem cells

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Embodiment Construction

[0031]Step 1. KGF-2 pretreatment: The route of administration of KGF-2 is intrapulmonary local administration, which is directly administered by instillation through a non-invasive artificial airway. After the rat was anesthetized, it was fixed on a plate of appropriate size, the mouth was opened, and the tracheal intubation was quickly placed about 1 cm below the glottis after the glottis was seen. In order to avoid damage to the local mucosa, the movement should be quick and gentle. After the intubation was completed, the rat was placed obliquely at an angle of 60 degrees to the ground, and the rhKGF-2 aqueous solution with a concentration of 2 mg / ml was slowly dripped into the airway through the tracheal intubation, and the dose was 5 mg rhKGF-2 / kg body weight. After injection, inject 2ml of air into the airway, repeat the air injection for a total of 3 times, so that the liquid in the lumen can be fully distributed to the small airway. After the infusion was completed, the...

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Abstract

The invention provides a method for extracting pulmonary mesenchymal stem cells. The method for extracting the pulmonary mesenchymal stem cells is characterized by comprising the following steps: administering a keratinocyte growth factor-2 (KGF-2) or a keratinocyte growth factor (KGF) in a pulmonary alveolus so as to enrich the pulmonary mesenchymalstem cells; and performing pulmonary alveolus lavage, and separating and purifying the mesenchymal stem cells in the obtained lung lavage fluid so as to obtain the pulmonary mesenchymal stem cells. By the method for extracting pulmonary mesenchymal stem cells, the KGF-2 can be enriched in the pulmonary mesenchymal stem cells, so that extraction through the pulmonary alveolus lavage can be carried out conveniently, and the pulmonary mesenchymal stem cells can be extracted from a normal animal lung tissue; the method for extracting pulmonary mesenchymal stem cells has the advantages that the extracted cells are high in purity, good in activity and convenient to use and the like; and by the method for extracting pulmonary mesenchymal stem cells, the cell source for the clinical stem cell therapy can be richened and a new method for separating autologous pulmonary mesenchymal stem cells is provided.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting a large number of lung mesenchymal stem cells. Background technique [0002] Stem cell research has made rapid progress in the past 10 years. Stem cell therapy has shown good therapeutic effects on many animal disease models. For example, intratracheal instillation or intravenous injection of mesenchymal stem cells can significantly reduce acute lung injury caused by LPS or bacteria. Mesenchymal stem cells can also alleviate myocardial ischemia caused by myocardial infarction. In vivo studies have found that KGF-2 has an anti-fibrotic effect and has a protective effect on bleomycin-induced pulmonary fibrosis. In vitro studies have found that KGF-2 has a protective effect on DNA damage and apoptosis of alveolar epithelial cells induced by mechanical tension, and also has a protective effect on alveolar epithelial cell damage induced by peroxide and asbestos,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 白春学童琳宋元林
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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