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Asexual propagation method of liverwort

A kind of land money and local technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of troublesome movement, large space occupation, low breeding efficiency, etc., and achieve the effect of cost saving and space saving

Inactive Publication Date: 2013-07-10
LIYANG AOGU INFORMATION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because this method needs to prepare flowerpots, soil, etc., on the one hand, it takes up a lot of space and is troublesome to move, and on the other hand, the breeding efficiency is not high.

Method used

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  • Asexual propagation method of liverwort
  • Asexual propagation method of liverwort

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Prepare MS liquid medium. The specific formula is as follows:

[0032] Potassium nitrate 1900mg / l, ammonium nitrate 1650mg / l, potassium dihydrogen phosphate 170mg / l, magnesium sulfate 370mg / l, calcium chloride 440mg / l, potassium iodide 0.83mg / l, boric acid 6.2mg / l, manganese sulfate 22.3mg / l l, Zinc Sulfate 8.6mg / l, Sodium Molybdate 0.25mg / l, Copper Sulfate 0.025mg / l, Cobalt Chloride 0.025mg / l, Disodium EDTA 37.3mg / l, Ferrous Sulfate 27.8mg / l l, inositol 100mg / l, glycine 2mg / l, thiamine hydrochloride 0.1mg / l, pyridoxine hydrochloride 0.5mg / l, niacin 0.5mg / l.

[0033] (2) Obtain cultured tissue blocks

[0034] The material is ground money (M.polymorpha). Cut the fronds of the ground money into 2×2mm tissue pieces. Use a coarse-meshed soil sieve to screen out tissue pieces that are too small.

[0035] (3) Disinfection of tissue blocks

[0036] First, wash the chopped tissue pieces with tap water, then soak them in 70% alcohol on the ultra-clean bench for 1 minut...

Embodiment 2

[0042] (1) Prepare B5 liquid medium. The specific formula is as follows:

[0043] Potassium nitrate 2500mg / l, magnesium sulfate 250mg / l, calcium chloride 150mg / l, ammonium sulfate 134mg / l, potassium iodide 0.75mg / l, boric acid 3.0mg / l, manganese sulfate 10mg / l, zinc sulfate 2.0mg / l, Sodium molybdate 0.25mg / l, cobalt chloride 0.025mg / l, copper sulfate 0.025mg / l, disodium edetate 37.3mg / l, ferrous sulfate 27.8mg / l, inositol 100mg / l, tobacco Acid 1.0mg / l, pyridoxine hydrochloride 1.0mg / l, ammonium sulfate hydrochloride 10mg / l.

[0044] (2) Obtain cultured tissue blocks

[0045]It is based on the ground money (M. cuneiloba). Cut the fronds of the ground money into 2×2mm tissue pieces. Use a coarse-meshed soil sieve to screen out tissue pieces that are too small.

[0046] (3) Sterilization of tissue blocks

[0047] First, wash the chopped tissue pieces with tap water, then soak them in 70% alcohol on the ultra-clean bench for 1 minute, and then sterilize them with 0.1% mercur...

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Abstract

The invention provides a novel method for propagating liverwort. According to the method, the liverwort is propagated by using a liquid culture medium; a plastic bracket is arranged in the liquid culture medium, so that tissue blocks of the liverwort cannot be immersed in the culture medium; and the tissue blocks of the liverwort are managed respectively before or after the rhizoids grow, and therefore, the aim of the rapid and efficient propagation of the liverwort is achieved.

Description

technical field [0001] The invention relates to a method for breeding ground money, in particular to a method for asexual breeding of ground money. Background technique [0002] Moss belongs to the bryophytes, and from the perspective of plant taxonomy, it belongs to the phylum Bryophyta, the class Moss, the order, the family, and the genus. [0003] The ground money is used as medicine as a whole, and it is mild in taste and cool in nature, and enters the spleen and lung meridians. It has the effects of dispelling heat, astringent, replenishing qi, clearing and tonifying, and improving eyesight. It can treat fire eye night blindness, scald burns, cough due to lung heat and chronic dysentery and rectal prolapse. Scientists from Israel's Weizmann Research have found that a component contained in the ground money can inhibit the activity of acetylcholinesterase in the human brain, thereby producing a curative effect on Alzheimer's disease. [0004] As a food, it has a signi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杜春林
Owner LIYANG AOGU INFORMATION TECH
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