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Calcium-complex starch-based microporous haemostatic material, and preparation method and application thereof

A hemostatic material, starch-based technology, applied in the fields of medical science, bandages, absorbent pads, etc., can solve the problems of difficult in vivo degradation, high preparation cost, poor biocompatibility, etc., and achieve good biocompatibility, easy preparation, and low cost. low effect

Active Publication Date: 2012-07-04
苏州佰济生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to overcome the shortcomings of current hemostatic materials such as poor biocompatibility, difficulty in in vivo degradation, and high production cost, and provide a calcium complexed starch-based microporous hemostatic material and its preparation method and application

Method used

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  • Calcium-complex starch-based microporous haemostatic material, and preparation method and application thereof
  • Calcium-complex starch-based microporous haemostatic material, and preparation method and application thereof
  • Calcium-complex starch-based microporous haemostatic material, and preparation method and application thereof

Examples

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preparation example Construction

[0062] Preparation method of starch-based microporous hemostatic material

[0063] The preparation method of the starch-based microporous hemostatic material of the present invention includes the following steps:

[0064] (1) Preparation of microporous starch

[0065] Add starch and enzyme to phosphate buffer, stir at 45-80℃ and pH 5-7 for enzymatic hydrolysis reaction, 4-15 hours later, centrifuge and dry to obtain microporous starch; wherein,

[0066] The mass ratio of starch and enzyme is 1: (0.01-0.2);

[0067] The mass-volume ratio of the total amount of starch and enzyme to the phosphate buffer is 100g: (250-1000) ml;

[0068] (2) Carboxymethylation of microporous starch

[0069] Dissolve monochloroacetic acid in ethanol, add sodium hydroxide solution to pH 6.5~7, mix it into the microporous starch prepared in step (1) of ethanol dissolving, stir well, and at 50-80℃ Continue stirring and heat preservation to react for 4-8 hours, add acetic acid to neutralize the pH to 6.5-7, filter...

Embodiment 1

[0110] First, weigh 100g tapioca starch and 1g glucoamylase, add to 250ml, pH=5 phosphate buffer solution, stir well, carry out hydrolysis reaction at 45℃ for 4 hours, centrifuge at 4000r / min, and Vacuum drying for 24 hours to obtain microporous starch.

[0111] Dissolve 8g of monochloroacetic acid in ethanol, adjust it to neutrality with 0.3mol / L sodium hydroxide solution, mix it into the microporous starch prepared above dissolved with 80g of ethanol, stir well and transfer it to a constant temperature bath. Under the condition of 50℃, the reaction was carried out with continuous stirring and heat preservation at 45r / min. After 4 hours, the reaction was completed, acetic acid was added to neutralize the pH to 6.5, filtered, washed with ethanol 3 times, and dried for 24 hours to obtain carboxymethyl microporous starch.

[0112] Finally, the carboxymethylated microporous starch obtained in the above reaction was added to a saturated calcium chloride solution, complexed and assembl...

Embodiment 2～4

[0114] Examples 2 to 4 repeat the experimental steps of Example 1, except that some experimental conditions are shown in Table 1-3.

[0115] The pure microporous starch prepared in Example 1 was observed by scanning electron microscope (SEM) (see attached figure 2 (a) and (b)) and the microstructure of the calcium complex microporous starch prepared in Example 2 and Example 4 (see attached figure 2 (c) and 2(d)). It can be seen from the figure that the prepared calcium-complexed microporous starch particles have a diameter of about 10-20 μm, and the surface is covered with small holes with a diameter of about 1-5 μm, and the pores are embedded from the surface to the center to form a cavity.

[0116] The average pore size of the calcium-complexed microporous starch prepared in Examples 1 to 4 is shown in Table 4. It can be seen from Table 4 that with the prolongation of the enzymatic hydrolysis reaction time, the average pore diameter of the calcium-complexed microporous starch o...

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Abstract

The invention relates to a calcium-complex starch-based microporous haemostatic material, and a preparation method and application thereof. The preparation method comprises the following steps of: firstly, preparing microporous starch loaded with carboxymethyl; secondly, complexing and assembling calcium ions; and finally, preparing the calcium-complex starch-based microporous haemostatic material, which has the pore diameter of 0.1-5um, the haemostatic time of 20 seconds to 30 minutes and can be completely degraded within 8 hours to 6 days. The cost of raw materials is low; the preparation method is simple; the obtained haemostatic material combines the physical haemostatic function of the microporous starch and the chemical haemostatic function of the carboxymethyl and the calcium ions, so that the haemostatic material is quick in haemostasis, high in efficiency, large in viscosity and good in biocompatibility, can be degraded in a body, and can be used for stopping bleeding of bleeding places such as human epidermis and internal tissues, massive bleeding places or active places of substantial organs in the body, and places where bleeding is difficult to control.

Description

Technical field [0001] The invention belongs to the field of tissue damage repairing medical materials, and particularly relates to a calcium-complexed starch-based microporous hemostatic material, and a preparation method and application thereof. Background technique [0002] Blood has the function of nourishing and nourishing all tissues and organs of the body, and is one of the basic substances that constitute the human body and maintain human life activities. Bleeding from trauma is a common occurrence in human life. Acute hemorrhage and deep hemorrhage of substantial organs caused by wars, traffic accidents, natural disasters, etc. are the main causes of death, disability and deformity after human injuries. When the traumatic blood loss reaches more than 20% of the total blood volume, obvious symptoms of shock appear; when it reaches 40% of the total blood volume, life is in danger. According to reports, during the War to Resist US Aggression and Aid Korea, nearly one-third...

Claims

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Application Information

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IPC IPC(8): A61L15/28A61L15/44A61L15/42C08B31/12
Inventor 刘昌胜陈芳萍陈晓龙魏杰
Owner 苏州佰济生物科技有限公司
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