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Injectable artemisia pollen allergen vaccine and preparation method thereof

A technology for allergens and injections, which is applied in the direction of allergen antigen components, respiratory diseases, skin diseases, etc. It can solve the problems of insignificant curative effect and long treatment cycle, so as to reduce medical burden, shorten treatment cycle, improve Efficacy and Safety Effects

Active Publication Date: 2014-09-10
北京新华联协和药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0024] However, compared with subcutaneous injection of immune desensitization, sublingual administration of immune desensitization has a longer treatment cycle, and the curative effect is not significant in a short period of time. The average desensitization cycle is 3 to 5 years

Method used

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  • Injectable artemisia pollen allergen vaccine and preparation method thereof
  • Injectable artemisia pollen allergen vaccine and preparation method thereof
  • Injectable artemisia pollen allergen vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Preparation of Artemisia grandis pollen allergen stock solution

[0055] 1) Collect the pollen of Artemisia grandis through mechanical (vacuum cleaner) collection or hydroponics, and then use a 300-mesh sieve to remove impurities such as dust, and conduct purity testing, morphological and molecular biology identification on the collected pollen.

[0056] 2) Add the pollen obtained in 1) to acetone at a volume ratio of 1:5, and degrease under intermittent stirring at room temperature for 3 hours each time.

[0057] 3) Add 1,000 g of defatted Artemisia grandis pollen to 25,000 ml of 200 mM pH 8.0 disodium hydrogen phosphate-sodium dihydrogen phosphate buffer at a feed ratio of 1:25 (W:V), and stir at 4°C for 24 hours.

[0058] 4) Centrifuge the extracted extract at 6000rpm at 4°C for 15min. Take the supernatant after centrifugation at 8000rpm at 4°C for 30min.

[0059] 5) Clarify and filter the centrate after centrifugation with an 8 μm filter membrane, and th...

Embodiment 2

[0062] Example 2 Preparation of Artemisia annua pollen allergen stock solution

[0063] 1) The pollen of Artemisia annua was collected by mechanical (vacuum cleaner) collection or hydroponic method, and then impurities such as dust were removed using a 300-mesh screen, and the collected pollen was tested for purity, morphological and molecular biological identification.

[0064] 2) Add the pollen obtained in 1) to acetone at a volume ratio of 1:5, and degrease under intermittent stirring at room temperature for 3 hours each time.

[0065] 3) Add 1,000 g of defatted Artemisia annua pollen to 25,000 ml of 200 mM pH 8.0 disodium hydrogen phosphate-sodium dihydrogen phosphate buffer at a feed ratio of 1:25 (W:V), and stir at 4° C. for 24 hours.

[0066] 4) Centrifuge the extracted extract at 6000rpm at 4°C for 15min. Take the supernatant after centrifugation at 8000rpm at 4°C for 30min.

[0067] 5) Clarify and filter the centrate after centrifugation with an 8 μm filter membrane...

Embodiment 3

[0070] Example 3: Preparation of mugwort pollen allergen stock solution

[0071] 1) Collect mugwort pollen by mechanical (vacuum cleaner) collection or hydroponics, and then use a 300-mesh sieve to remove impurities such as dust, and conduct purity testing, morphological and molecular biology identification on the collected pollen.

[0072] 2) Add the pollen obtained in 1) to acetone at a volume ratio of 1:5, stir intermittently at room temperature for degreasing, each time for 3 hours, until the supernatant acetone is colorless after degreasing, and dry the degreasing solid naturally until there is no smell of acetone After weighing.

[0073] 3) Add 1000 g of degreased mugwort pollen to 25000 ml of 200 mM disodium hydrogen phosphate-sodium dihydrogen phosphate buffer at a feed ratio of 1:25 (W:V), and stir at 4° C. for 24 hours.

[0074] 4) Centrifuge the extracted extract at 6000rpm at 4°C for 15min. Take the supernatant after centrifugation at 8000rpm at 4°C for 30min.

...

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Abstract

The invention relates to an injectable artemisia pollen allergen vaccine and a preparation method thereof. The injectable artemisia pollen allergen vaccine contains artemisia pollen allergens and an aluminum adjuvant, wherein the artemisia pollen allergens comprise artemisia sieversiana pollen allergens, sweet wormwood pollen allergens and argy wormwood pollen allergens. The injectable artemisia pollen allergen vaccine has obvious effects of treating hypersensitivity diseases such as allergic dermatitis, chronic urticaria, allergic rhinitis and allergic asthma.

Description

technical field [0001] In the field of biological technology, the invention specifically relates to a vaccine for injection of Artemisia pollen allergens and a preparation method thereof. Background technique [0002] People's understanding of allergic diseases began with "hay fever". In 1911, scientists Noon and Freeman used pollen extract to treat hay fever for the first time, and the history of allergen disease treatment began from then on. The development of allergens in my country was introduced from the United States by Professor Zhang Qingsong, director of the Department of Otorhinolaryngology of Union Medical College Hospital in 1956, and made a breakthrough. He discovered that the most important autumn-type allergenic pollen in China is Artemisia plant pollen. China's own allergens provide a basis for realizing the localization of allergens. In the 1980s, Ye Shitai and Qiao Bingshan led dozens of hospitals across the country to conduct a survey of airborne allergeni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/35A61P11/02A61P11/06A61P17/00A61P37/08
Inventor 白彩明裴潇竹姜敏汤承祁何立峰牛占坡乔秉善
Owner 北京新华联协和药业有限责任公司
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