Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Stable liquid kit for measuring lactic acid

The technology of a kit and a stabilizer is applied in the field of lactic acid determination liquid kits, which can solve the problems such as the decrease of the pH value of the reagent, the poor stability of the reagent bottle opening, the stability of the reagent affecting the measurement accuracy, and the effect of promoting the reaction.

Active Publication Date: 2012-06-20
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
View PDF6 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because hydrazine easily reacts with carbon dioxide in the air to form carbazinic acid, the stability of this method is poor when opening the bottle. After the reagent is opened, the pH value of the reagent will soon drop due to the absorption of carbon dioxide in the air, which will affect the accuracy of the measurement. degree and stability of reagents

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Stable liquid kit for measuring lactic acid
  • Stable liquid kit for measuring lactic acid
  • Stable liquid kit for measuring lactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The components and concentrations of reagent 1 are:

[0031] Imidazole buffer (pH 5.5) 0.3 M

[0032] α-Naphthol 20 mg / L

[0033] NAD 20 mg / L

[0034] Sodium azide 0.2 g / L

[0035] The components and concentrations of reagent 2 are:

[0036] Piperazine-1,4-diethanesulfonic acid buffer (pH 8.5) 0.1M

[0037] Lactate dehydrogenase 50 KU / L

[0038] Ethylene glycol 10g / L

[0039] Proclin300 0.5 g / L.

[0040] The preparation method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and then mixed and stirred evenly.

Embodiment 2

[0042] The components and concentrations of reagent 1 are:

[0043] Citric acid-trisodium citrate buffer (pH 6.0) 0.1 M

[0044] β-naphthol 20 mg / L

[0045] thio-NAD 50 mg / L

[0046] Proclin300 0.1 g / L

[0047] The components and concentrations of reagent 2 are:

[0048] Tris buffer (pH 9.0) 0.2 M

[0049] Lactate dehydrogenase 80 KU / L

[0050] Bovine serum albumin 0.1 g / L

[0051] Proclin300 1g / L

[0052] The preparation method of the kit in Example 2 is the same as in Example 1.

Embodiment 3

[0064] Example 3 The preparation method of the kit is the same as that in Example 1.

[0065] The test conditions for the kit of the present invention to measure lactic acid in a sample are as follows: temperature: 30-37°C; light path of cuvette: 1.0 cm. The main detection wavelength is 340 nm, and the secondary wavelength is 405 nm.

[0066] The method for measuring lactic acid in a sample by using the lactic acid determination kit of the present invention is as follows: add R1 to the sample (calibration tube as the sample) and mix well, incubate at 30-37°C for 3-5 minutes, read the absorbance A0, immediately add R2 and mix well , After reacting at 30-37°C for 5 min, read the absorbance A1, ΔA=A1-A0. The amount of sample used is 3 μl, the amount of reagent 1 is 200 μl, and the amount of reagent 2 is 50 μl.

[0067] The LAC content in the test kit of the present invention is calculated according to the following formula:

[0068]

[0069] The performance of the kit obtaine...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a stable liquid kit for measuring lactic acid, characterized in that the liquid kit is liquid double-reagent composed of a reagent 1 and a reagent 2; the ingredients and concentrations of the reagent 1 are as follows: 0.05-0.5 M of buffering solution with pH of 5.5-6.5, 20-200 mg / L of alpha / beta-naphthol, 20-200 mg / L of coenzyme and 0.1-10 g / L of preservative; the ingredients and concentrations of the reagent 2 are as follows: 0.05-0.5 M of buffering solution with pH of 8.5-9.5, 20-200 KU / L of lactate dehydrogenase, 0.1-100 g / L of stabilizer and 0.1-10 g / L of preservative. The liquid kit of the invention has the advantages of good accuracy and precision and good bottle opening stability, and the liquid kit can be stably stored for at least 15 days if being opened at 2-8 DEG C, so that the liquid kit can satisfy the clinical examination demand.

Description

technical field [0001] The invention belongs to the technical field of medical examination and measurement, and in particular relates to a stable liquid kit for lactic acid measurement. Background technique [0002] Lactic acid is an intermediate product of sugar metabolism, mainly derived from skeletal muscle, brain, skin, renal medulla and red blood cells, etc. The concentration of lactic acid in the blood is related to the rate of lactic acid produced by these tissues and the metabolism rate of lactic acid by the liver. About 65% of lactic acid Utilized by the liver, while extrahepatic lactate is cleared by oxidation of skeletal muscle and renal cortex. It is generally believed that when the lactic acid concentration exceeds 5 mmol / L and the pH value is less than 7.25, it indicates obvious lactic acidosis. Lactic acidosis occurs in two clinical situations: type A (hypoxic): common and associated with decreased tissue oxygenation, such as shock, hypovolemia, and left vent...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52
Inventor 邹炳德沃燕波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com