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Stable liquid kit for measuring lactic acid

A technology of kits and reagents, applied in the field of medical testing and determination, can solve the problems such as the decrease of pH value of reagents, poor stability of reagents when opening bottles, and stability of reagents affecting the accuracy of measurement, and achieve the effect of promoting the reaction.

Active Publication Date: 2014-01-01
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because hydrazine easily reacts with carbon dioxide in the air to form carbazinic acid, the stability of this method is poor when opening the bottle. After the reagent is opened, the pH value of the reagent will soon drop due to the absorption of carbon dioxide in the air, which will affect the accuracy of the measurement. degree and stability of reagents

Method used

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  • Stable liquid kit for measuring lactic acid
  • Stable liquid kit for measuring lactic acid
  • Stable liquid kit for measuring lactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The components and concentrations of reagent 1 are:

[0031] Imidazole buffer (pH 5.5) 0.3 M

[0032] α-Naphthol 20 mg / L

[0033] NAD 20 mg / L

[0034] Sodium azide 0.2 g / L

[0035] The components and concentrations of reagent 2 are:

[0036] Piperazine-1,4-diethanesulfonic acid buffer (pH 8.5) 0.1M

[0037] Lactate dehydrogenase 50 KU / L

[0038] Ethylene glycol 10 g / L

[0039] Proclin300 0.5 g / L.

[0040] The preparation method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and then mixed and stirred evenly.

Embodiment 2

[0042] The components and concentrations of reagent 1 are:

[0043] Citric acid-trisodium citrate buffer (pH 6.0) 0.1 M

[0044] β-Naphthol 20 mg / L

[0045] thio-NAD 50 mg / L

[0046] Proclin300 0.1 g / L

[0047] The components and concentrations of reagent 2 are:

[0048] Tris buffer (pH 9.0) 0.2 M

[0049] Lactate dehydrogenase 80 KU / L

[0050] Bovine serum albumin 0.1 g / L

[0051] Proclin300 1 g / L

[0052] The preparation method of the kit of Example 2 is the same as that of Example 1.

Embodiment 3

[0064] Example 3 The preparation method of the kit is the same as in Example 1.

[0065] The test conditions for determining the lactic acid in the sample by the kit of the present invention are as follows: temperature: 30-37°C; and the optical path of the cuvette is 1.0 cm. The main wavelength of detection is 340 nm and the secondary wavelength is 405 nm.

[0066] The method of using the lactic acid determination kit of the present invention to determine lactic acid in a sample is as follows: the sample (calibration tube uses the calibrator as the sample) is mixed with R1, the absorbance A0 is read after incubating at 30-37°C for 3-5 min, and R2 is added immediately to mix. After reacting at 30-37°C for 5 minutes, read the absorbance A1, ΔA=A1-A0. The sample volume is 3 μl, the reagent 1 volume is 200 μl, and the reagent 2 volume is 50 μl.

[0067] The LAC content in the sample determined by the kit of the present invention is calculated according to the following formula:

[0068]...

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Abstract

The invention discloses a stable liquid kit for measuring lactic acid, characterized in that the liquid kit is liquid double-reagent composed of a reagent 1 and a reagent 2; the ingredients and concentrations of the reagent 1 are as follows: 0.05-0.5 M of buffering solution with pH of 5.5-6.5, 20-200 mg / L of alpha / beta-naphthol, 20-200 mg / L of coenzyme and 0.1-10 g / L of preservative; the ingredients and concentrations of the reagent 2 are as follows: 0.05-0.5 M of buffering solution with pH of 8.5-9.5, 20-200 KU / L of lactate dehydrogenase, 0.1-100 g / L of stabilizer and 0.1-10 g / L of preservative. The liquid kit of the invention has the advantages of good accuracy and precision and good bottle opening stability, and the liquid kit can be stably stored for at least 15 days if being opened at 2-8 DEG C, so that the liquid kit can satisfy the clinical examination demand.

Description

Technical field [0001] The invention belongs to the technical field of medical testing and determination, and specifically relates to a stable liquid kit for determination of lactic acid. Background technique [0002] Lactic acid is an intermediate product of sugar metabolism, mainly derived from skeletal muscle, brain, skin, renal medulla and red blood cells. The concentration of lactic acid in the blood is related to the rate of lactic acid production by these tissues and the rate of metabolism of lactic acid by the liver, about 65% of lactic acid It is used by the liver, and extrahepatic lactic acid is eliminated by oxidation of skeletal muscle and renal cortex. It is generally believed that when the lactic acid concentration exceeds 5 mmol / L and the pH value is less than 7.25, it indicates obvious lactic acidosis. Lactic acidosis occurs in the following two types of clinical conditions: Type A (hypoxic type): common and related to reduced tissue oxygenation, such as shock, h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/32
Inventor 邹炳德沃燕波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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