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Preparation method and application of magnetic fluorescent nanoparticle with shell-core structure

A fluorescent nanoparticle and nanoparticle technology, applied in the fields of biomedicine and nanomaterials, can solve the problems of easy quenching of fluorescence, rough surface of nanoparticles, poor uniformity, etc.

Active Publication Date: 2012-06-20
SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nanoparticles prepared by traditional methods such as self-assembly and co-precipitation have defects such as rough surface, poor uniformity, and easy quenching of fluorescence.

Method used

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  • Preparation method and application of magnetic fluorescent nanoparticle with shell-core structure

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Experimental program
Comparison scheme
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Embodiment 1

[0017] The preparation of embodiment 1 superparamagnetic nanoparticles

[0018] Weigh 2.71g FeCl 3 ·6H 2 O and 1.18 g FeCl 2 4H 2 O was dissolved in 50ml of distilled water. After stirring and mixing, 4mL of concentrated ammonia water was added, and stirred at room temperature for 30min. Under the action of a magnetic field, the product was collected and the supernatant was removed. The product was washed 3 times with deionized water, 200 mL each time. The particles were made up to 50 mL with deionized water.

Embodiment 2

[0019] Example 2 Fe 3 o 4 SiO 2 Preparation of nanoparticles

[0020] Take 7.5mL cyclohexane, 1.77mL Triton X-100, and 1.8mL n-hexanol in a 10mL flask, mix well; add 480μL Fe 3 o 4 Aqueous solution, stirring for 5 minutes to form water-in-oil microcapsules, then add 100 μL TEOS, stir and mix, add 60 μL ammonia water; wrap in tin foil, react for 24 hours; add an equal volume of acetone, vortex, break the microemulsion system; place in a magnetic field , let stand for a while, discard the liquid; wash the particles with acetone, absolute ethanol, and water in sequence, 50 mL / time, and vortex and ultrasonically break the particles during the washing process. The particles were resuspended in 50mL of 1M hydrochloric acid and allowed to stand at room temperature for 24h. Place in a magnetic field, let it stand for a while, and discard the liquid; wash the particles with deionized water and absolute ethanol three times, 50 mL each time; resuspend the particles in 20 mL of absol...

Embodiment 3

[0021] Embodiment 3 coated rare earth fluorescent complex

[0022] Add 6.3 μL triaminopropyltriethoxysilane to 50 μL 0.1M BHHCT solution, vortex and mix well, let stand at room temperature for 20 minutes, add 50 μL 0.1M EuCl 3 solution, mix well, and stand at room temperature for 20min. This liquid is transferred to the Fe obtained in embodiment 2 3 o 4 SiO 2 In absolute ethanol suspension, protected from light, reflux at 80°C for 18h. Place in a magnetic field, let it stand for a while, and discard the liquid; wash the particles 3 times with deionized water and absolute ethanol, 50 mL each time. After the particles were dried in a vacuum oven at 60° C. for about 12 hours, magnetic nanoparticles coated with fluorescent complexes were obtained.

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Abstract

The invention relates to a preparation method and an application of a magnetic fluorescent nanoparticle with a shell-core structure. Firstly, a silica magnetic microsphere with a shell-core structure is prepared by using one or more than one of nanoparticles of Fe3O4, gamma-Fe2O3, MeFe2O4 (Me=Co, Mn, Ni), metal Ni, Co, Fe, and alloy Fe-Co, Ni-Fe as the inner core, and coating a silica shell, and then a fluorescent material (a chelate of Eu3+, Sm3+, Dy3+, Tb3+ and the like) is absorbed on the silica shell. Then, a layer of silica is coated on the surface to improve the stability of the fluorescent magnetic microsphere, and to prevent agglomeration and fluorescent material leakage. A lot of rare earth fluorescent materials are wrapped in the shell layer, so the fluorescence intensity signal of a prepared sample is greatly increased. The nanoparticle has dual functions of enrichment and marking, and has wider application prospects in the biomedical field.

Description

【Technical field】 [0001] The invention belongs to the technical fields of nanomaterials and biomedicine, and in particular relates to the preparation and application of a magnetic fluorescent composite nanoparticle. 【Background technique】 [0002] In the field of modern biomedical research, labeling and enrichment separation are essential steps. Rare earth fluorescent nanoparticles have labeling advantages such as long fluorescence lifetime, large Stokes shift, broad excitation spectrum and sharp emission peak. They have gradually replaced traditional organic fluorescent dyes and are used in the fields of biomarkers and medical diagnosis, but they do not have the functions of collection and separation. Magnetic nanoparticles have the characteristics of good superparamagnetism and easy functionalization of the surface. They can combine with the target to be separated and concentrate under the action of an external magnetic field, but they do not have the function of labeling ...

Claims

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Application Information

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IPC IPC(8): B01J13/02C09K11/06H01F1/00
Inventor 朱海李富荣蒋原李金峰王西丽
Owner SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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