Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of molecularly imprinted solid phase extraction small column for separating pyrethroid pesticide

A technology of pyrethroids and solid-phase extraction cartridges, applied in the field of analysis and detection, to achieve the effects of low cost, high selectivity and sensitivity, and high purification efficiency

Inactive Publication Date: 2012-06-20
NINGBO UNIV
View PDF2 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, although MISPE technology has been successfully applied to the separation and purification of various pesticide residues, it mainly focuses on the research of a single pesticide residue, and most of them are designed based on the specific separation of a single target compound. In the water environment and aquatic organisms, Pyrethroid pesticides mostly exist in the form of mixtures, so the research on molecularly imprinted polymers for multi-residue recognition of pyrethroid pesticides has important application value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of molecularly imprinted solid phase extraction small column for separating pyrethroid pesticide
  • Preparation method and application of molecularly imprinted solid phase extraction small column for separating pyrethroid pesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of molecularly imprinted polymers for pyrethroid pesticides:

[0023] Cypermethrin is used as a template molecule, methacrylic acid (MAA) is used as a functional monomer, ethylene glycol dimethacrylate (EGDMA) is used as a crosslinking agent, and the molar ratio of template molecule: functional monomer: crosslinking agent is 1:5 : 25, template molecules, functional monomers, cross-linking agents, initiators and porogens were added into the reactor, and bulk molecularly imprinted polymers were obtained after polymerization by bulk polymerization. The ratio of the initiator to the crosslinking agent is 4 mg / mmol; the volume ratio of the porogen to the crosslinking agent is 1:10.

[0024] Grind and sieve the obtained massive molecularly imprinted polymers to obtain particles with a particle size of about 40 μm. The template molecules are removed by Soxhlet extraction using a mixed solution of methanol formic acid and methanol with a volume ratio of 9:1, and the...

Embodiment 2

[0036] Preparation of molecularly imprinted polymers for pyrethroid pesticides:

[0037]With cypermethrin as template molecule, methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linking agent, according to template molecule: functional monomer: cross-linking agent molar ratio=1:2 : 25, template molecules, functional monomers, cross-linking agents, initiators and porogens were added into the reactor, and bulk molecularly imprinted polymers were obtained after polymerization by bulk polymerization. The ratio of the initiator to the crosslinking agent is 3 mg / mmol; the volume ratio of the porogen to the crosslinking agent is 1:6.

[0038] The obtained massive molecularly imprinted polymer was ground and sieved to obtain particles with a particle size of 50 μm, and the template molecules were removed by Soxhlet extraction using a mixed solution of methanol, formic acid and methanol at a volume ratio of 9:1, and then placed in a vacuum dr...

Embodiment 3

[0050] Preparation of Molecularly Imprinted Polymers for Pyrethroid Pesticides

[0051] With cypermethrin as template molecule, methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linking agent, according to template molecule: functional monomer: cross-linking agent molar ratio=1:8 : 25, template molecules, functional monomers, cross-linking agents, initiators and porogens were added into the reactor, and bulk molecularly imprinted polymers were obtained after polymerization by bulk polymerization. The ratio of the initiator to the crosslinking agent is 5 mg / mmol; the volume ratio of the porogen to the crosslinking agent is 1:16.

[0052] The block molecularly imprinted polymer obtained was ground and sieved to obtain particles with a particle size of 60 μm, and the template molecules were removed by Soxhlet extraction using methanol: formic acid (9:1, v / v) and methanol, and the obtained molecularly imprinted polymer was further polyme...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method and application of a molecularly imprinted solid phase extraction small column for separating a pyrethroid pesticide. The molecularly imprinted solid phase extraction small column for separating the pyrethroid pesticide is characterized by consisting of cypermethrin as template molecules, methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent according to a mol ratio of 1:(2-8):10; and a pyrethroid pesticide molecularly imprinted polymer obtained with a mass polymerization method is filled into a polypropylene solid phase extraction small column to obtain the molecularly imprinted solid phase extraction small column for separating the pyrethroid pesticide. According to the invention, the pyrethroid pesticide in an aquatic product or a water environmental sample can be efficiently and selectively separated, enriched and purified. Compared with the liquid-liquid extraction and the conventional solid phase extraction, the molecularly imprinted solid phase extraction column disclosed by the invention has the advantages of high enriching and purifying efficiency, low consumption of organic solvent and the like.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to a preparation method and application of a molecularly imprinted solid-phase extraction column for separating pyrethroid pesticides. Background technique [0002] Pyrethrin pesticides are the third generation of new pesticides developed in my country. Due to their high efficiency and low toxicity, they are widely used in agricultural production. However, these pesticides are highly toxic to aquatic organisms such as fish and crabs, resulting in The mortality rate is high, causing great harm to fishery production. At the same time, due to the increasingly serious environmental problems caused by the large-scale use of chemical pesticides, the pesticides entering the environment can easily enter rivers or coastal seas with the surface runoff formed by rainwater, causing pollution to the water environment of coastal seas, and eventually lead to Such pesticides in commercially av...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/22G01N1/34
Inventor 史西志孙爱丽李德祥刘菁华陈炯
Owner NINGBO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products