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Diterpene compound kalihinol and use thereof

A technology for compounds and diterpenes, applied in the field of diterpenoids, can solve the problem of no anti-fouling active diterpenoids and the like, and achieve the effect of obvious anti-fouling activity

Inactive Publication Date: 2014-07-02
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But so far there is no report on the new diterpenoid kalihinol T with anti-fouling activity isolated from the sponge A. cavernosa

Method used

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  • Diterpene compound kalihinol and use thereof
  • Diterpene compound kalihinol and use thereof
  • Diterpene compound kalihinol and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1. prepare diterpenoid kalihinol T of the present invention

[0018] 1. Preparation of Total Extract

[0019] Take the South China Sea sponge A.cavernosa with a wet weight of 5.5 kg, use 10 times the volume of acetone solution to leak and extract, combine the extracts, and recover the solvent under reduced pressure to obtain a crude extract;

[0020] 2. Separation and purification

[0021] The crude extract was dissolved in water, extracted three times with dichloromethane, the extract was dispersed in 60%-90% methanol and extracted three times with petroleum ether and dichloromethane successively, and the solvent was recovered under reduced pressure to obtain the petroleum ether fraction (49g) and Dichloromethane fraction (30g) extract; put the dichloromethane extract on silica gel decompression column chromatography as usual, use petroleum ether-acetone system at 100:1-50:1-30:1-15:1-10: 1-5:1-1:1 for gradient elution, according to the difference in polar...

Embodiment 2

[0027] Embodiment 2: Antifouling activity test of the compound of the present invention

[0028] The most harmful fouling organism barnacle B. amphitrite larvae were selected to establish the anti-fouling activity screening model. Adult barnacles were collected from the intertidal zone of Hong Kong (22°19'N, 114°16'E) and fed on Chaetoceros gracilis, and were cultured from nauplii to Venus larvae in 0.22-μm-filtered seawater stage, the culture temperature is 28°C, and the anti-fouling activity of Venus stage larvae is tested.

[0029] The test compound kalihinol T was dissolved in dimethyl sulfoxide to form a series of concentration gradients (0.5-10 μg / ml), and sterile filtered seawater was used as the control group. Take about 20 barnacle larvae and put them into a 24-well polystyrene plate, add 1ml of test solution to each well, and make 5 replicate samples for each concentration, and place the 24-well cell culture plate in a 30°C incubator for 24 hours. Observe the numbe...

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Abstract

The invention relates to the field of anti-fouling agent, specifically a novel diterpene compound kalihinol which is acquired by extracting from South China Sea sponge Acanthella caveronosa and a use thereof. The diterpene compound kalihinol has a structure shown as the formula (I). The compound can remarkably restrain barnacle Balanus amphitrite larva adsorption, which indicates that the compound has remarkable fouling resistance activity through in vitro barnacle Balanus amphitrite larva adsorption resistance activity test. Therefore, the diterpene compound kalihinol can be used for preparing a novel anti-fouling agent. The invention provides a novel source for preparing an environment-friendly high-efficient anti-fouling agent.

Description

technical field [0001] The invention relates to the field of antifouling agents, in particular to a new diterpene compound kalihinol T extracted and separated from the sponge Acanthella cavernosa and its application. Background technique [0002] Marine fouling organisms are a general term for marine animals, plants and microorganisms that grow on the bottom of ships and on the surface of artificial facilities in the sea. The proliferation of fouling organisms will cause great harm to ships and artificial facilities in the sea, such as increasing ship resistance, clogging pipelines, accelerating metal corrosion, causing failure of instruments and components in the ocean, and threatening aquaculture. The annual cost of preventing biofouling of artificial facilities in the sea alone is as high as 6.5 billion US dollars. At present, the use of antifouling coatings is the most economical and widely used method to prevent marine pollution. Organotin antifouling agents, such as t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/14A01N47/46A01P7/00A01P13/00A01P1/00
Inventor 林厚文徐影刘香芳焦伟华郎俊慧于豪兵朴淑娟陈万生
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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