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Gene chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment

A gene chip and interferon technology, which is applied in the field of chronic hepatitis C prognosis prediction gene chip, can solve the problems of standardization and mass production, which are difficult to overcome, and achieve the effects of facilitating clinical application, reducing treatment costs, and reducing pain.

Inactive Publication Date: 2012-06-06
PEOPLES HOSPITAL PEKING UNIV
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  • Abstract
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Problems solved by technology

This method can greatly increase the lattice density, and the binding amount of each probe on the surface is relatively consistent, but there are still difficulties in standardization and mass production.

Method used

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  • Gene chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment
  • Gene chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment
  • Gene chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment

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Experimental program
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Effect test

Embodiment

[0036] 10ml of anticoagulated blood was taken from 12 patients with chronic hepatitis C before treatment with long-acting interferon plus ribavirin, and peripheral blood mononuclear cells were separated by Ficoll density gradient centrifugation. The separated mononuclear cells were equally divided into control group and experimental group. Group. The control group was cultured with DMEM medium for 48 hours, and the experimental group was treated with DMEM medium containing 100 U / ml IFNα for 48 hours, and the mRNA of the above samples was extracted.

[0037] Detection with gene chips. The change value of the relative expression level of ISG between the experimental group and the control group was obtained, and the total score of ISG was evaluated according to the above-mentioned scoring standard (results are shown in Table 2). in:

[0038] The total ISG expression score of the gene chip in 5 patients was 31-36 points (group 1), predicting that the probability of obtaining a s...

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Abstract

The invention relates to a gene chip for chronic hepatitis C outcome prediction in chronic hepatitis C interferon treatment. The gene chip comprises 12 gene fragments of ISG56, ISG20 and the like. Through the gene chip, in an early stage of chronic hepatitis C interferon treatment on patients or before the chronic hepatitis C interferon treatment on patients, interferon treatment long-term effects can be predicted. Therefore, the gene chip is conducive to optimization and selection of a chronic hepatitis C patient therapeutic schedule, reduces patient pain and treatment costs, and is convenient for detection and clinical application.

Description

【Technical field】 [0001] The invention relates to a gene chip, in particular to a chronic hepatitis C outcome prediction gene chip. 【Background technique】 [0002] Hepatitis C virus (HCV) infection is one of the most important viral infectious diseases at present. About 170 million people in the world are chronically infected with HCV, and chronic HCV infection may further develop into liver cirrhosis or even liver cancer. As there is currently no effective vaccine, there are still three to four million new HCV infections each year. Interferon-alpha (IFN-α) combined with ribavirin is currently the most effective treatment, but it requires subcutaneous injection of medication, the medication cycle is very long, the cost of treatment is expensive, and there are many adverse factors such as side effects of drugs; and interference The sustained virologic response to HCV after antibiotic therapy is only about 50%. Therefore, the discovery of factors that can predict the progno...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C40B40/06
Inventor 潘孝本魏来
Owner PEOPLES HOSPITAL PEKING UNIV
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