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Novel in-vitro diagnosis experiment method and device for allergen

An experimental method, the technology of allergens, applied in the field of medical experiments, can solve the problems of inability to detect at the same time

Inactive Publication Date: 2012-05-30
SINNOWA MEDICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing allergen detection methods cannot detect specific IgE and IgG at the same time 4

Method used

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  • Novel in-vitro diagnosis experiment method and device for allergen
  • Novel in-vitro diagnosis experiment method and device for allergen
  • Novel in-vitro diagnosis experiment method and device for allergen

Examples

Experimental program
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Effect test

Embodiment Construction

[0008] Prepare immunobeads or compliant micromagnetic beads made of avidin-coated iron blocks sandwiched by plastic materials. Anti-human IgE polyclonal or monoclonal antibody labeled with horseradish peroxidase (HRP) and anti-human IgG with alkaline phosphatase (ALP) 4 Polyclonal or monoclonal antibodies, labeled with biotin for various allergen protein antigens. During detection, add patient detection serum (specific IgE and IG4), biotin-labeled allergen protein antigen, enzyme-labeled anti-human IgE and IG4 antibody, and then add avidin-labeled solid-phase carrier immune beads into the reaction tube , incubate at 37°C for about 30 minutes, wash to remove unbound immune complexes, absorb immune beads, one for each cup, and put them in two cuvettes (cis-compliant micro-magnetic beads can take one-third of the total amount, and quantify them separately) Add) and then add two different enzyme-catalyzed chemiluminescence substrates, HRP and ALP, respectively, into the cuvette. ...

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Abstract

The invention provides an in-vitro diagnosis experiment method and device for detecting different human antibodies (IgE and IgG4) specific to multiple allergens, and belongs to medical experiment field. The method adopts horseradish peroxidase (HRP) and alkaline phosphatase (ALP) to label anti-human IgE antibody and anti-human IgG4 antibody respectively, and combines them with solid phase carrier in the same immuno-reaction system. After incubation and washing, equal amounts of the solid phase carriers are placed in colorimetric reaction cuvettes respectively, and then chemiluminescence substrates catalyzed by different enzymes (HRP and ALP) are used to detect luminescence intensity. The method can simultaneously detect IgE antibody and IgG4 antibody of testee specific to multiple allergens. The matched device comprises immuno-bead loading structure system, washing system, and immuno-bead / micro-magnetic-bead transfer system. The device has the functions of controlling immuno-bead addition amount, transferring immuno-bead, quantitatively adding and transferring micro-magnetic-bead, etc.

Description

Technical field: [0001] The invention is a method for simultaneously detecting the specific IgE and IgG produced by the human body to various allergens. 4 The in vitro diagnostic test method and device for different antibodies, the method and supporting device can be applied to a fully automatic allergen detection system to achieve large-scale, high-throughput, rapid and accurate detection of allergen antibodies in patients' serum, and timely diagnosis of diseases. It belongs to the field of medical experiments. Background technique: [0002] In recent years, the incidence of allergic diseases has been increasing year by year. Every year, more than 20% of people suffer from allergic diseases. According to statistics, the incidence of this disease in my country is higher than that in developed countries. Early diagnosis of allergic diseases is very important, and the most critical thing is to find out the allergens that cause individual allergic reactions, and take targeted...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N21/76
Inventor 王礼文宋成桥徐新
Owner SINNOWA MEDICAL SCI & TECH
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