Method for screening tumor specificity target and targeting ligand based on tissue chip

Abstract

The invention which belongs to the biotechnological field concretely relates to a method for screening a tumor specificity target and a targeting ligand with a tissue chip. The invention which aims to overcome disadvantages of low medicine or targeting treatment versatility, large gap between a screening system and in-vivo living environment of a tumor, small screening throughout, and time and labor consumption existing in the prior art provides a method for screening the tumor specificity target and the targeting ligand with the tissue chip. The targeting ligand screened in the invention has the advantages of high specificity, realization of tumor cell penetrating, no immunization reaction causing, and high versatility. The screening method maintains the original state of the in-vivo grown tumor; and compared with tumor-bearing mouse screening systems, the screening method has the advantages of animal breeding step omitting, time, labor saving and cost saving, and strong experiment result comparability. Compared with traditional screening systems, the screening method of the invention allows the tumor specificity target analysis and the targeting research to be simultaneously carried out, and the verification process is simple.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for screening tumor-specific targets and targeting ligands based on tissue chips. Background technique [0002] Tumor has become one of the intractable diseases that seriously threaten human health, and is surpassing cardiovascular disease to become the number one cause of death for human beings. The traditional treatment methods for tumors are mainly surgery, radiotherapy and chemotherapy. In addition to resecting and destroying tumor tissues, these treatment methods also cause great damage to normal tissues and cells due to their non-targeting. [0003] Targeted therapy is an important progress in the field of modern tumor therapy. On the basis of tumor molecular cell biology, it uses specific structural molecules possessed by tumor tissues or cells as targets, and uses certain drugs that can specifically bind to these target molecules. Antibodies, ligands, e...

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the disadvantages in the prior art of low versatility of drugs or targeted therapy, large gap between the screening system and the living environment of tumors in vivo, small screening throughput, time-consuming and laborious, and provide a tissue chip-based tumor-specific Screening methods for sexual targets and targeting ligands

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