Extraction method of Cinnamomum camphora seed oil by ultrasound-assisted compound enzyme
A technology of camphor seeds and compound enzymes, which is applied in the direction of fat oil/fat production and fat production, and can solve the problems of quality impact, multiple equipment, and long extraction process
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[0004] Below in conjunction with example the present invention is described in further detail.
[0005] Ultrasonic-assisted compound enzyme extraction of camphor seed oil specific implementation: camphor seed→peeling→drying→crushing→adding buffer solution→adding compound enzyme→adding petroleum ether→ultrasonic treatment→oil separation→right oil ether recovery→vacuum drying → Camphor Seed Oil.
[0006] (1) Pretreatment: camphor seeds remove moldy seeds and other impurity components, remove the exocarp after cleaning, and treat at a temperature of 40-45°C for 36-48 hours to reduce the moisture content of camphor seeds to about 10%, and then The pulverizer is crushed and passed through a 60-80 mesh sieve for later use;
[0007] (2) Buffer preparation: according to the action conditions of the compound enzyme (cellulase+amylase), prepare a buffer solution of pH 4.5;
[0008] (3) Enzymolysis: Add a certain amount of compound enzyme (cellulase + amylase) solution to the buffer so...
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