Method for perstraction of fermented microbial intracellular product with non-ionic surfactant

Abstract

The invention discloses a method for perstraction of a fermented microbial intracellular product with a non-ionic surfactant. Specifically, during fermentation of a microbial intracellular product, a non-ionic surfactant micellar solution formed by addition of the non-ionic surfactant concentration or a cloud point system formed thereby is adopted as a microbial fermentation medium, by which the intracellular product can be penetrated to an extracellular environment so as to improve the product level of microbial fermentation. Simultaneously, perstraction and fermentation of the microbial intracellular product can be realized. The method of the invention is especially suitable for the fermentation process of an intracellular product, such as production of intracellular microbial enzymes, production of intracellular organic small molecule substances as well as production of intracellular oil compounds, etc. The method provided in the invention effectively eliminates intracellular product inhibition, enhances the concentration of a microbial fermentation product, and adjusts the composition of a microbial fermentation secondary metabolite, thus improving the volume yield of microbial fermentation as well as the efficiency of intracellular product fermentation, product release and other processes.

Description

technical field [0001] The invention relates to a method for extracting intracellular products of fermented microorganisms, in particular to a method for permeating and extracting intracellular products of fermented microorganisms with a nonionic surfactant. Background technique [0002] Microbial fermentation intracellular products usually accumulate high product concentrations in the cells, resulting in product inhibition. The main solution is to increase the product concentration by increasing the cell density of microorganisms. On the other hand, the accumulation of intracellular products makes it necessary to use methods such as cell disruption and solvent extraction in the downstream separation and purification process, which increases the complexity of the process and operating costs. Using fermentation in a water-organic solvent two-phase system to change the permeability of the cell membrane can release the intracellular products to the extracellular environment, bu...

AI Technical Summary

Problems solved by technology

Wang Z, Dai Z. Extractive microbial fermentation in cloud point system. Enzyme Microbial Technology, 2010, 46: 407-418], but the application of non-ionic surfactant solution and the cloud point system formed in the process of microbial fermentation to change the cell membrane The so-called osmoextraction fermentation technique of osmolarity to release intracellular products while extracting products has not been reported yet.

Images