Acetic acid bacterium fermentation nutritive salt and use method thereof
A technology of acetic acid bacteria and nutrient salt, which is applied in the field of nutrient salt solution and fermented nutrient salt, can solve the problems of low cost performance, unclear ingredients, unstable fermentation effect, etc., and achieve the goal of improving the quality of acetic acid, reducing production costs, and shortening the fermentation cycle Effect
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Embodiment 1
[0020] Embodiment 1: Nutrient salt is dissolved in water according to ratio 1 and carries out acetic acid batch fermentation
[0021] 11 Preparation of seed medium:
[0022] Glucose and hydrolyzed yeast powder were dissolved in deionized water at a content of 55% (w / v) and 45% (w / v) respectively, adjusted to pH 6.5, and sterilized at 121° C. for 20 minutes.
[0023] 1.2 Activation and expansion of acetic acid bacteria
[0024] The strains preserved on the slant were activated in a constant temperature incubator at 30°C for 24 hours, and one loop of the strains preserved on the slope was inoculated into a 250mL Erlenmeyer flask with 50mL of seed medium, and cultured with shaking at 120r / min and 30°C for 24h on a shaker. The activated acetic acid bacteria are expanded and cultivated step by step, and after three stages of expansion, it is the seed liquid for acetic acid fermentation.
[0025] 1.3 Preparation of acetic acid bacteria fermentation nutrient salt
[0026] The diff...
Embodiment 2
[0036] Embodiment 2: Nutrient salt is dissolved in water according to ratio 2 and carries out acetic acid batch fermentation
[0037] 2.1-2.2 The preparation method of acetic acid bacteria seed solution is the same as that in Example 1
[0038] 2.3 Preparation of acetic acid bacteria fermentation nutrient salt
[0039] The different components of acetic acid bacteria fermentation nutrient salt are prepared according to ratio 2: 27% (weight) hydrolyzed yeast powder, 16% (weight) ammonium citrate, 45% (weight) glucose, 5% (weight) dipotassium hydrogen phosphate, 4% (weight) disodium hydrogen phosphate, 6% (weight) magnesium sulfate.
[0040] 2.4 Acidification of fermented mash and alcoholization
[0041] Dissolve the prepared nutrient salt in water 12 hours before fermentation according to the addition amount (w / v) of 5‰, add 1% (v / v) liquid fermented vinegar and 5% (v / v) edible alcohol before fermentation, and prepare into acetic acid fermentation mash.
[0042] 2.5 Acetic ...
Embodiment 3
[0049] Embodiment 3: Nutrient salt is dissolved in water according to ratio 3 and carries out acetic acid batch fermentation
[0050] 3.1-3.2 The preparation method of acetic acid bacteria seed solution is the same as that in Example 1
[0051] 3.3 Preparation of nutrient salt fermented by acetic acid bacteria
[0052] The different components of acetic acid bacteria fermentation nutrient salt are prepared according to ratio 3: 26.5% (weight) hydrolyzed yeast powder, 15.5% (weight) ammonium citrate, 44.8% (weight) glucose, 4.2% (weight) dipotassium hydrogen phosphate, 3.7% (weight) disodium hydrogen phosphate, 5.3% (weight) magnesium sulfate.
[0053]3.5 Acidification of fermented mash and alcoholization
[0054] Dissolve the prepared nutrient salt in water 12 hours before fermentation according to the addition amount (w / v) of 3‰, add 1% (v / v) liquid fermented vinegar and 5% (v / v) edible alcohol before fermentation, and prepare into acetic acid fermentation mash.
[0055] ...
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