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Method for detecting heavy metal by utilizing molecular device based on graphene electrode

A graphene electrode, graphene technology, applied in the field of detection of heavy metals, can solve the problem of lack of sensitivity

Active Publication Date: 2012-05-02
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are more and more researches on heavy metal detection methods, mainly including immunoassay, atomic spectrophotometry, stripping voltammetry, chromatography, test paper method, enzyme analysis method, biochemical sensor method, etc. Some existing methods lack Higher sensitivity is greatly affected by environmental factors, and it is urgently needed to establish a system of highly sensitive detection methods

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  • Method for detecting heavy metal by utilizing molecular device based on graphene electrode
  • Method for detecting heavy metal by utilizing molecular device based on graphene electrode
  • Method for detecting heavy metal by utilizing molecular device based on graphene electrode

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Example 1: Detection of trace amounts of Cu by DNAzyme molecular devices based on graphene electrodes 2+

[0055] Detection of Cu 2+ DNAzyme molecular devices, including:

[0056] a) array of graphene transistor devices, each graphene transistor device in the array of graphene transistor devices includes gate, source, drain and conduction channel, and the conduction channel is graphene; wherein, in the graphene transistor device array The graphene is provided with a passage obtained by etching graphene by oxygen plasma, and the passage is provided with a molecular connection site (nano-gap) with a length of 1-10 nm at intervals, and the molecular connection site is connected to the graphene. The source and drain in the transistor device are vertical; wherein, the gate is a silicon substrate containing a silicon dioxide layer with a thickness of 300nm, and its resistivity is 5-20ohm·cm -1 The source electrode and the drain electrode are composed of a Cr electrode laye...

Embodiment 2

[0074] Example 2: Graphene electrode-based DNAzyme molecular device detects trace amounts of pb 2+

[0075] detect pb 2+ The DNAzyme molecular device, in addition to the DNAzyme connected at the molecular junction site and the detection of Cu 2+ The DNAzyme molecular devices are different, but the rest are the same.

[0076] testing pb 2+ The DNAzyme connected in the DNAzyme molecular device is a DNA with a double-stranded pocket conformation formed by the following sequence c and sequence d, pb 2+ Able to specifically cleave specific sites of the DNA sequence.

[0077] c.H 2 N-(CH 2 ) 3 -5′-GTAGAGAAGGrATATCACTCA-3′-(CH 2 ) 3 -NH 2 (The nucleotide sequence is shown in sequence 3 in the sequence listing)

[0078] d.5'-TGAGTGATAAAGCTGGCCGAGCCTTCTTCTCTAC-3' (the nucleotide sequence is shown in sequence 4 in the sequence listing)

[0079] Among them, the "r" in the sequence c represents that A is ribonucleic acid; the 1-9 bases from the 5' end of the sequence c are com...

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Abstract

The invention discloses a method for detecting heavy metal and a DNAzyme monomolecular connection device based on a graphene electrode special for same. A manufacturing method for the DNAzyme monomolecular connection device comprises the following steps of: 1) constructing a graphene transistor device array; 2) cutting a nanometer gap of 1-10nm in a graphene molecular device; and 3) connecting a designed DNAzyme sequence into the nanometer gap to obtain the DNAzyme monomolecular connection device. An electrical signal detection with heavy metal ion binding and identification is carried out byutilizing the manufactured DNAzyme monomolecular connection device, meanwhile, the interference factors are excluded; the heavy metal ions are tested in sensitivity and the like on the connection device; and the measurement conditions are determined according to the test results in the above steps, and the heavy metal ions are detected on the connection device. According to the invention, the high-sensitivity detection of the heavy metal can be realized through the electricity signal by utilizing the functionalized graphene electrode molecular device. The method is of great significance in detecting a trace amount of the heavy metal in the environment.

Description

technical field [0001] The invention relates to a method for detecting heavy metals using a graphene electrode-based molecular device. Background technique [0002] Heavy metals can not only exist for a long time, but also can be stored in organisms through the accumulation of food chains. The toxicity produced is difficult to detect. Moreover, the pollution of heavy metals is becoming more and more serious, which has attracted people's attention in medicine, food, environment and other aspects. At present, there are more and more researches on heavy metal detection methods, mainly including immunoassay, atomic spectrophotometry, stripping voltammetry, chromatography, test paper method, enzyme analysis method, biochemical sensor method, etc. Some existing methods lack Higher sensitivity is greatly affected by environmental factors, and it is urgently needed to establish a system of highly sensitive detection methods. (1: Malitesta C, Guascito M R. Biosensor Bioelectron, 200...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30C12Q1/44B81C1/00B81B7/04
Inventor 郭雪峰高力
Owner PEKING UNIV
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