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Culture medium and culture method for cultivating immature embryo of double-petal paeonia lactiflora

A technique for culturing immature embryos and a culture medium, which is applied in the field of medium formulations for culturing immature embryos of Paeonia suffruticosa, can solve the problems of poor development of embryos of Paeonia japonicas, failure of normal sowing and emergence, and the like, so as to overcome technical obstacles and shorten the breeding cycle. , the effect of increasing growth rate

Inactive Publication Date: 2012-05-02
YANGZHOU UNIV
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Problems solved by technology

[0005] In order to solve the technical problem that the double-flowered peony can not be sown and sprouted normally due to poor embryonic development, the present invention provides a medium formula for cultivating young embryos of double-flowered peony, and a method for cultivating peony seedlings using the medium. Hybrid breeding provides key technical support

Method used

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  • Culture medium and culture method for cultivating immature embryo of double-petal paeonia lactiflora
  • Culture medium and culture method for cultivating immature embryo of double-petal paeonia lactiflora
  • Culture medium and culture method for cultivating immature embryo of double-petal paeonia lactiflora

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Embodiment 1

[0027] The double-petalled peony variety 'Hongyan Zhenghui' was used as the test material to carry out this research work:

[0028] (1) Materials: Collect the pods of 'Hongyan Zhenghui' whose skin has started to change color in the first ten days of July, soak them in washing solution for 10 minutes, rinse them with tap water, soak them in 1% for 20 minutes, rinse them with tap water, and peel off the pods. pods, remove the seeds, first sterilize the surface with 75% alcohol for 30 s, then sterilize with 0.1% mercuric chloride for 8 min, and rinse with sterile water 5 to 6 times. Place on sterile filter paper, cut open the seeds with a scalpel, and pick out the seed embryos with a dissecting needle.

[0029] (2) Culture medium formula:

[0030] ① Inoculate the seed embryos in the following three media for inducing adventitious buds: 1), improved MS + TDZ 0.5 mg / L, sucrose 100g L -1 , agar 6g·L -1 , pH 5.8; 2), modified MS + TDZ 1.0 mg / L, sucrose 100g L -1 , agar 6g·L -1...

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Abstract

The invention specifically relates to a culture medium prescription for cultivating immature embryo of double-petal paeonia lactiflora and a method for cultivating the seedlings of the paeonia lactiflora by using the culture medium. The culture medium comprises adventitious bud induction culture medium, adventitious bud strengthening culture medium and rooting culture medium; and the culture medium is formed by adding TDZ, cane sugar and agar based on improved MS or 1 / 2 improved MS. The culture method comprises the following steps: selecting the seedling embryo; preparing the culture medium; and inoculating to cultivate, wherein the step of inoculating to cultivate is the step of adopting the three culture mediums to induce the adventitious bud, strengthen the adventitious bud and root. Because of the invention, the seedling rate of the immature embryo reaches over 60%, and the technical obstacle that the double-petal paeonia lactiflora cannot establish the seedlings caused by the dysplasia of the seedling embryo. Each germination seedling embryo can establish 3-8 adventitious buds and 5-10 fleshy roots, so the effect that the seedlings of the normal seed differentiate the buds after three years is realized, the growth speed of the seedlings of the paeonia lactiflora is improved, the hybrid seedlings of the paeonia lactiflora flowers at early stage, and the breeding period of the paeonia lactiflora is shortened.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a medium formula for cultivating immature embryos of peony double petals and a method for cultivating peony seedlings using the medium. Background technique [0002] The existing excellent ornamental varieties of peony in my country are mainly obtained through natural seed selection, and the books about the introduction of peony varieties do not indicate the source of their parents. Although many scientific research units have carried out hybrid breeding of peony through the selection and mating of paeoniae officinalis parents, the progress has been slow for many years. The reason is that the peony ( Paeonia lactiflora Pall.) The most important double-petal trait in the ornamental quality is formed by the petalization variation after the degeneration of the pistil or stamen. Therefore, it is difficult to fertilize and set fruit when using double-petal varieties of peony with ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陶俊薛银芳赵大球葛金涛韩晨霞
Owner YANGZHOU UNIV
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