Method for extracting, separating and purifying enramycin

A technology of enramycin and extract, which is applied in the field of biochemical industry, can solve the problems of complex production operation, low purity, and affecting economic benefits, and achieve the effect of simple operation and short time

Active Publication Date: 2012-03-21
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the enramycin product produced in the prior art has lower purity, dark color, lower product yield and complex production operations, thus affecting economic benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Streptomyces fungicidicus is used as the production strain, and its spore liquid is preserved in a liquid nitrogen environment; the frozen spore liquid is inoculated into the seed medium for seed cultivation to obtain a seed liquid; then the seed liquid is inoculated Carry out fermentation culture in fermentation medium, obtain brown yellow enramycin fermented liquid (enramycin content 5.54g / L, total sugar content 0.98%, reducing sugar content 0.34%, protein content 0.39%, dissolving phosphorus 78mg / L L).

[0028] 1) Take 10L brown-yellow enramycin fermentation broth, heat it to 60°C, concentrate in vacuum until the enramycin content is 55.4g / L, filter it through a 300,000D ceramic membrane, and centrifuge it at 5000rpm in a centrifuge. To obtain 1000 g of mycelium, add 4 L of 50% methanol (volume ratio), stir and break under ultrasonic conditions for 3 h, and filter to obtain enramycin methanol extract.

[0029] 2) Adjust the pH of the enramycin methanol extract to 2....

Embodiment 2

[0035] Streptomyces fungicidicus is used as the production strain, and its spore liquid is preserved in a liquid nitrogen environment; the frozen spore liquid is inoculated into the seed medium for seed cultivation to obtain a seed liquid; then the seed liquid is inoculated Carry out fermentation culture in fermentation medium, obtain brown yellow enramycin fermentation liquid (enramycin content 5.01g / L, total sugar content 0.89%, reducing sugar content 0.51%, protein content 0.54%, dissolving phosphorus 103mg / L L).

[0036] 1) Take 10L brown-yellow enramycin fermentation liquid, heat it to 55°C, concentrate in vacuum until the enramycin content is 50.1g / L, filter through a 500,000D ceramic membrane, and centrifuge at 4500rpm with a centrifuge. To obtain 1200 g of mycelium, add 4 L of 55% methanol (volume ratio), stir and break under ultrasonic conditions for 4 h, and filter to obtain enramycin methanol extract.

[0037] 2) Use 15% hydrochloric acid to adjust the pH of the en...

Embodiment 3

[0043] Streptomyces fungicidicus is used as the production strain, and its spore liquid is preserved in a liquid nitrogen environment; the frozen spore liquid is inoculated into the seed medium for seed cultivation to obtain a seed liquid; then the seed liquid is inoculated Carry out fermentation culture in fermentation medium, obtain brown yellow enramycin fermented liquid (enramycin content 4.92g / L, total sugar content 0.93%, reducing sugar content 0.46%, protein content 0.43%, dissolving phosphorus 93mg / L L).

[0044] 1) Take 10L brown-yellow enramycin fermentation broth, heat it to 65°C, concentrate in vacuum until the enramycin content is 49.2g / L, filter it through a 100,000D ceramic membrane, and centrifuge it with a centrifuge at 5000rpm. To obtain 1000 g of mycelium, add 5 L of 45% methanol (volume ratio), stir and break under ultrasonic conditions for 6 h, and filter to obtain enramycin methanol extract.

[0045] 2) Adjust the pH of the enramycin methanol extract to ...

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PUM

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Abstract

The invention relates to a method for extracting, separating and purifying enramycin from enramycin fermentation liquor. The method comprises the following steps of: performing heat treatment on the enramycin fermentation liquor, filtering, centrifuging, and collecting thalli; adding a methanol solution into the thalli, performing ultrasonic treatment, and filtering; acidifying and alkalizing filtrate, decoloring, adsorbing a decoloring solution by using macroporous adsorption resin, eluting by using methanol, collecting eluent, performing concentration and crystallization, and performing freeze-drying to obtain the enramycin. The method for extracting the enramycin has the advantages that: the required time is short, the product purity is high, the process is simple, and the operating cost is low.

Description

technical field [0001] The invention relates to a method for extracting and purifying enramycin from fermented liquid of enramycin, which belongs to the field of biochemical industry. Background technique [0002] Enramycin is an organic base composed of 17 amino acid molecules and fatty acid molecules of 13 different types. Among them, the amino acid molecules form a cyclic polypeptide structure, and the fatty acid is located at the end of the polypeptide structure. According to the different types of terminal fatty acids, it is divided into enramycin A and enramycin B, and enramycin is a mixture of these two components. Enramycin hydrochloride is a white crystalline powder with a molecular weight of about 2500 and a melting point of 238-245°C. It is easily soluble in dimethyl sulfoxide, soluble in methanol and aqueous ethanol, hardly soluble in acetone, and insoluble in benzene. , Chloroform. Enramycin hydrochloride has excellent stability to heat, light and humidity. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/64C07K1/36C07K1/34C07K1/22
Inventor 李荣杰张雪锋魏生
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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