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Polypeptide for specifically targeting lung cancer cell, and preparation method and application thereof

A technology of lung cancer cells and targeting peptides, which is applied in the fields of biology and medicine, and achieves the effect of simple preparation method and broad application prospects

Active Publication Date: 2012-03-21
BIOCELLS BEIJING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacterial surface display technology combined with flow cytometry technology has become a hotspot in the field of display technology due to its fast and high-throughput characteristics. Although researchers at home and abroad are deepening the targeted research on lung cancer, so far There are no reports on targeting molecules that can specifically target lung cancer cells and methods for specific detection of lung cancer cells

Method used

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  • Polypeptide for specifically targeting lung cancer cell, and preparation method and application thereof
  • Polypeptide for specifically targeting lung cancer cell, and preparation method and application thereof
  • Polypeptide for specifically targeting lung cancer cell, and preparation method and application thereof

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Embodiment Construction

[0020] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings and a preferred embodiment.

[0021] The A-13A-1 peptide of this example is screened through the following steps:

[0022] 1) In vitro screening of bacterial random peptide library:

[0023] To recover the random peptide library of bacteria, take at least 10 times the amount of bacteria in the library in LB medium, expand and grow at 37°C, and induce bacteria with 0.02% (m / v) arabinose at room temperature for 1 hour, according to the ratio of bacteria:cells 100:1 Ratio and HLF cell incubation of lung fibroblasts, 4°C, 30min, centrifuge at 1000rpm, 4°C, 5min, take the supernatant and incubate with the same amount of lung cancer cells A549 cells, 4°C, 30min, centrifuge at 1000rpm, 4°C, 5min, 3 The supernatant was discarded, resuspended in PBS (pH7.4), and the binding status was analyzed by flow cytometry. The cells bound to the bacteria were sorte...

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Abstract

The invention discloses polypeptide for specifically targeting a lung cancer cell and an application thereof. The polypeptide for specifically targeting the lung cancer cell has an amino acid sequence shown as SEQ ID NO.1, and is prepared by screening on the surface of the human lung cancer cell by an ex-vivo bacterial random polypeptide library exhibiting method and a flow cell sorting method. The polypeptide for specifically targeting the lung cancer cell can be applied to preparation of lung cancer diagnosing tracer, lung cancer cell detecting reagent and the like. The polypeptide for specifically targeting the lung cancer cell can be combined with a lung cancer A549 cell specifically, is not combined with a normal lung cell and other tumor cells; and the preparation method is simple, feasible and applicable to large-scale industrial production. The polypeptide for specifically targeting the lung cancer cell provides important theoretical and practical basis for early diagnosis, targeting therapy of the lung cancer and the like, and has a wide application prospect.

Description

technical field [0001] The invention relates to a cancer cell targeting polypeptide and its preparation method and application, in particular to a lung cancer cell-specific targeting polypeptide and its preparation method and application, belonging to the technical fields of biology and medicine. Background technique [0002] Lung cancer is one of the most frequently occurring tumors in the world. According to statistics, lung cancer has surpassed liver cancer to become the first cause of death from malignant tumors in my country, accounting for 22.7% of all malignant tumor deaths, and the morbidity and mortality continue to rise rapidly. Modern medicine has made a lot of progress in the scientific research of lung cancer, but the early diagnosis and effective treatment of lung cancer are still difficult problems in the process of diagnosis and treatment. How to improve the sensitivity of early diagnosis and the accuracy of treatment has become the goal of lung cancer treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08G01N33/68
Inventor 朱毅敏董兵
Owner BIOCELLS BEIJING BIOTECH CO LTD
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