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Separation and purification method for safflower yellow

A technology for the separation and purification of safflower yellow pigment, which is applied in the field of natural medicine extraction, can solve the problems of poor product purity, easy moisture absorption, and difficult storage, and achieve the effects of less reagent consumption, easy operation, and less pollution

Inactive Publication Date: 2012-03-21
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

The traditional preparation process of safflower yellow is extraction with water and refining by organic solvent precipitation, that is, safflower-crushing-distilled water extraction to obtain extract solution-refining filtration-filtrate concentrated under reduced pressure-precipitation of concentrated solution-centrifugal separation-vacuum drying- Crushing - finished product, but the product obtained by this method has poor purity, which contains impurities such as protein, sugar, salt, etc., is easy to absorb moisture, and is not easy to store

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  • Separation and purification method for safflower yellow
  • Separation and purification method for safflower yellow
  • Separation and purification method for safflower yellow

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Embodiment Construction

[0020] The following describes the specific embodiments of the present invention but does not limit the present invention.

[0021] The separation and purification process of the present invention is through the key parameters in the separation and purification process: sample loading conditions, cleaning conditions, and elution conditions are investigated, and finally the best separation and purification method of safflower yellow of the present invention is obtained, which improves the concentration of solids. Contents of total safflower yellow and hydroxysafflower yellow A.

[0022] 1. Materials and Instruments

[0023] Safflower: purchased from Hehuachi Traditional Chinese Medicine Market in Chengdu, identified by Professor Pei Jin as the dried flower of Compositae safflower (Carthamus tinctorius L.); hydroxysafflower yellow A reference substance: tested by HPLC, the purity is ≥99%; LD605, D140, D141, D100, D160 macroporous adsorption resins are provided by China Blue Che...

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Abstract

The invention belongs to the field of natural medicine extraction, and particularly relates to a separation and purification method for safflower yellow, which is used for obtaining solid matters with total safflower yellow content more than 96%. The separation and purification method for safflower yellow utilizes safflowers as raw materials and utilizes water as dissolve for extracting, and extractives are separated and purified by means of LX-68 type macro-porous absorption resin, so that the total safflower yellow content and hydroxy safflower yellow A content contained in the solid matters are more than 96% and more than 16% respectively, and the separation and purification method for safflower yellow is simple and convenient in operation, and low in reagent consumption and pollution.

Description

technical field [0001] The invention belongs to the field of natural medicine extraction, and in particular relates to a method for separating and purifying safflower yellow. Background technique [0002] Safflower is the dried flower of Carthamus tinctorius L., a plant of Compositae. Safflower yellow, also known as safflower yellow, referred to as SY in the present invention, is the main effective component of safflower, and exists in the water-soluble extraction part of safflower. Hydroxy safflower yellow A, referred to as HSYA in the present invention, has a relatively high content in safflower yellow, and has anti-tumor, anti-coagulation, inhibition of thrombosis, anti-oxidation, anti-inflammation, and lipid-lowering effects [1-4] . [0003] The content of safflower yellow in safflower varies greatly, and the extraction and purification process of safflower yellow has always been a hot issue in safflower research. The traditional preparation process of safflower yello...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H7/04C07H1/08C09B61/00
Inventor 裴瑾万德光梁颖张选
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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