Method for purifying high-content karanjin

A technology of aquaxanthin and a purification method, which is applied in the field of natural medicinal phytochemistry and achieves the effects of strong feasibility, large adsorption capacity and high product purity

Inactive Publication Date: 2012-03-14
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no literature report on the extraction, separation and purification of anhydroxanthin

Method used

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  • Method for purifying high-content karanjin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take dried flower bean stems and branches as raw materials, weigh 1kg after crushing and put it into the percolation cylinder, add 7 times of 80% ethanol percolation extraction for 3 times, each time for 4 hours, combine the extracts; concentrate the extracts to 1 / 4 of the original volume , ultrasonically extracted twice with an equal volume of petroleum ether, discarded the extract, put the raffinate on an AB-8 macroporous adsorption resin column (diameter-to-height ratio 1:5), eluted with water until colorless, and then used 6 times column volume 70% ethanol was eluted, collected, the reagent was recovered under reduced pressure, concentrated, and the obtained extract was subjected to polyamide column chromatography, followed by ethyl acetate, ethyl acetate-methanol (9:1), ethyl acetate- Gradient elution with methanol (9:4), collecting the eluate, recovering the reagent, concentrating to dryness to obtain coarse crystals, and recrystallizing repeatedly with 95% ethanol...

Embodiment 2

[0021] Take dried flower bean stems and branches as raw materials, weigh 3.5kg after crushing and put it into an extraction tank, add 10 times of 70% ethanol and heat and reflux for extraction twice, each time for 3 hours, and combine the extracts; concentrate the extracts to 1 / 1 of the original volume 5. Use an equal volume of petroleum ether to ultrasonically extract twice, discard the extract, put the raffinate on a D101 macroporous adsorption resin column (diameter-to-height ratio 1:10), elute with water until colorless, and then use 5 80% of the column volume was eluted with methanol, collected, the reagent was recovered under reduced pressure, concentrated, and the obtained extract was subjected to polyamide column chromatography, followed by ethyl acetate, ethyl acetate-methanol (9:1), ethyl acetate-methanol (9:4) gradient elution, collect the eluate, recover the reagent, concentrate to dryness to obtain coarse crystals, and repeat recrystallization with 95% ethanol for ...

Embodiment 3

[0023] Take dried flower bean stems and branches as raw materials, weigh 5kg after crushing and put it into the extraction tank, add 8 times of 60% ethanol to cold soak and extract twice, each time for 4 hours, combine the extracts; concentrate the extracts to 1 / 7 of the original volume , ultrasonically extracted twice with an equal volume of petroleum ether, discarded the extract, put the raffinate on a NKA-9 macroporous adsorption resin column (diameter-to-height ratio 1:15), first eluted with water until colorless, and then used 8 times the column volume was eluted with 75% acetone, collected, the reagent was recovered under reduced pressure, concentrated, and the obtained extract was subjected to polyamide column chromatography, followed by ethyl acetate, ethyl acetate-methanol (9:1), ethyl acetate- Gradient elution with methanol (9:4), collecting the eluate, recovering the reagent, concentrating to dryness to obtain coarse crystals, and recrystallizing repeatedly with 95% ...

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Abstract

The invention relates to a method for purifying high-content karanjin, comprising the following steps: crushing cauline branches of fordia cauliflora hemsl, extracting and concentrating with ethanol having a certain concentration, extracting twice with petroleum ether, using macroporous resin on the extract, concentrating the eluent and separating through a polyamide column, and recrystillizing through 95 % ethanol. The method has the advantages of low production cost and simplicity, and is easy to batch production. The cauline branches are used as raw material, thus the invention has good resource advantage.

Description

Technical field: [0001] The invention relates to a method for purifying high-content lutein, which belongs to the field of natural medicinal phytochemistry. Background technique: [0002] Phantanthin (Kranjin); [0003] Molecular formula: C 18 h 12 o 4 ;Molecular weight: 292.29; [0004] Molecular formula: [0005] [0006] Physical and chemical properties: colorless needle crystal, mp.157~158℃, soluble in methanol, ethanol, chloroform, benzene, ether, concentrated sulfuric acid, nitric acid, acetic acid, hydrochloric acid, almost insoluble in petroleum ether. Phucanthin naturally exists in the roots and stems of Fordia cauliflora Hem sl., the root bark and seeds of the leguminous plant Pongamiapinnata (L.) Merr., and has anti-tuberculosis The role of bacilli can be used for the treatment of vitiligo. [0007] Dai Bin et al. obtained the crystalline substance xanthin from the chloroform-dissolved part of the alcohol extract that has a nootropic effect, and determin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04
Inventor 李法庆郭琴刘东锋
Owner 苏州宝泽堂医药科技有限公司
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