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Method for separating and purifying succinic acid from fermentation broth

A technology for separating and purifying and fermenting liquid, which is applied in the technical field of separating and purifying succinic acid from fermentation liquid, can solve the problems of poor economy, difficult coupling of fermentation processes, unreasonable unit operation design, etc., and achieves the effect of improving yield

Inactive Publication Date: 2012-02-29
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] In short, the current process has complicated process flow, poor economy, unreasonable unit operation design, difficult to realize coupling with fermentation process, and low yield of succinic acid, which needs to be further improved and improved

Method used

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  • Method for separating and purifying succinic acid from fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Using glucose as the carbon source, Actinobacillus succinate BE-1 is the succinic acid fermentation broth of the production strain, wherein the concentration of succinic acid is 45g / L, the concentration of formic acid is 8g / L, the concentration of acetic acid is 10g / L, and the concentration of lactic acid The concentration is 14g / L, the amount of residual sugar is less, and the pH of the fermentation broth is 6.8.

[0066] The fermented liquid is subjected to ultrafiltration operation. The ultrafiltration operation adopts hollow fiber membrane equipment. The membrane material is PES membrane. The membrane separation capacity is 10kDa. After filtering, the removal rate of protein was 91%. At this time, the concentration of succinic acid was 43g / L. D301 type resin was used to decolorize succinic acid. The decolorization temperature was 40°C. The concentration is 40g / L. The fermented liquid after clarification and decolorization is vacuum distilled and concentrated, the co...

Embodiment 2

[0069] Using glucose as the carbon source, Actinobacillus succinate BE-1 is the succinic acid fermentation liquid of the production strain, wherein the concentration of succinic acid is 52g / L, the concentration of formic acid is 9g / L, the concentration of acetic acid is 7g / L, and the concentration of lactic acid The concentration is 17g / L, the amount of residual sugar is less, and the pH of the fermentation broth is 6.6.

[0070] The fermentation broth is processed according to two schemes, scheme 1: fermentation broth—centrifugation—decolorization—vacuum distillation concentration—crystallization; scheme 2: fermentation broth—ultrafiltration—decolorization—vacuum distillation concentration—crystallization;

[0071] Option 1: Centrifuge the fermentation broth obtained above at a speed of 6000r / min for 30 minutes, collect the supernatant after centrifugation, and use D301 resin to decolorize succinic acid at a temperature of 40°C. After decolorization, the fermentation broth is ...

Embodiment 3

[0075] Using glucose as the carbon source, Actinobacillus succinate BE-1 is the succinic acid fermentation liquid of the production strain, wherein the concentration of succinic acid is 62g / L, the concentration of formic acid is 10g / L, the concentration of acetic acid is 6g / L, and the concentration of lactic acid The concentration is 18g / L, no residual sugar, and the pH of the fermentation broth is 6.8.

[0076] Two schemes are adopted for the clarification of the fermentation broth. Scheme 1: Microfiltration followed by ultrafiltration; Scheme 2: The fermentation broth is clarified directly by ultrafiltration.

[0077] Option 1: Microfiltration-ultrafiltration operation is performed on the fermentation broth at the same time for comparison. The microfiltration operation uses a hollow fiber microfiltration membrane, the pore size of the microfiltration membrane is 0.1 μm, the membrane material is PE membrane, and the cross-flow operation is adopted. method, the operating tempe...

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Abstract

The invention belongs to a technology for separating succinic acid from fermentation products, and specifically relates to a method for separating and purifying succinic acid from fermentation broth. According to the invention, separation and purification of succinic acid can be realized by the operations of appropriate units and effective combinations of the units, such that defects of existing succinic acid separation and purification technologies of complicated process and poor economical efficiency can be overcome. Therefore, a novel economic technology for separating and purifying succinic acid from fermentation broth is established. Specifically, the fermentation broth is clarified through ultrafiltration. Resin decoloration is used for replacing traditional active carbon decoloration, such that the final succinic acid yield can be improved. Succinic acid crystals are directly obtained through crystallization. With the technology, the purity of the separated succinic acid reaches 99.5%, and the yield is higher than 90%.

Description

technical field [0001] The invention relates to a production process of succinic acid, in particular to a process for separating and purifying succinic acid from a fermentation broth. Background technique [0002] Succinic acid (Succinic Acid), also known as succinic acid, widely exists in organisms and is an important dibasic organic carboxylic acid. As an important C 4 As a platform compound, succinic acid can participate in various types of reactions to synthesize a variety of chemical products such as 1,4-butanediol (BDO), γ-butyrolactone (GBL), tetrahydrofuran (THF), etc., so it is used in many It has a wide range of uses, such as a tranquilizer in the pharmaceutical field, a food additive in the food industry, and a surfactant. [0003] At present, the industrial production of succinic acid is obtained by hydrogenation catalysis of maleic anhydride obtained from fossil raw materials. The chemical synthesis method is increasingly exposed to many problems, such as the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C55/10C07C51/42C07C51/47C07C51/43
Inventor 邢建民王彩霞万印华
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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