Method for screening a fish liver protecting drug by using carp liver injury model induced by CCl4
A liver injury and drug technology, applied in the field of aquatic drug screening, can solve the problems of inability to evaluate the anti-liver injury activity of the tested drug, the inapplicability of fish liver disease prevention, and the death of fish due to hypoxia. Blood is easy and the individual is large
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Embodiment 1
[0024] Example 1 Acute liver injury modeling screening experiment
[0025] 1. CCl used to induce carp acute liver injury model 4 Choice of Concentration
[0026] Take 50 carps with a body weight of about 150 grams, and randomly divide them into control group and different concentrations of CCl 4 Groups (12.5%, 25%, 30% and 50%), 5 groups in total, 10 in each group, were raised in 5 aquariums of a recirculating aquaculture system, and the water volume of each aquarium was 150 liters. After feeding for 2 weeks, with concentrations of 0, 12.5%, 25%, 30% and 50% (v / v) CCl 4 Vegetable oil solution was injected intraperitoneally into the control group and different concentrations of CCl 4 group, the dose was 0.05mL / 10g fish body weight. After 72 hours of injection, the fish in each group were anesthetized with 3-aminobenzoic acid ethyl methylsulfonate MS222 (100mg / L), and 1.5mL of blood was collected from each tail vein, and the serum was centrifuged and produced by Nanjing Jian...
Embodiment 2
[0039] Example 2 uses CCl 4 Induced carp liver injury model to screen fish hepatoprotective drugs
[0040] Experimental method: Take 60 carps with a body weight of about 150 grams and raise them in 6 aquariums in a recirculating aquaculture system. The water volume of each aquarium is 150 liters, and 10 carps are raised. Randomly divided into normal control group, CCl 4 A model control group and a test drug group, wherein the test drug group includes a high-dose astragalus polysaccharide group, a low-dose astragalus polysaccharide group, a high-dose angelica extract group and a low-dose angelica extract group. Normal control group and CCl 4 The model control group was fed carp pellet feed, and the test drug group was fed carp pellet feed added with a certain dose of astragalus polysaccharide or angelica extract. The daily feeding amount was 3% of the fish body weight. After continuous feeding for 6 weeks, The normal control group was injected intraperitoneally with vegetabl...
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