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Preparation method of chitosan glycine anticoagulation derivative

A technology of chitosan glycine and derivatives, applied in the field of biomedicine, can solve the problems of high price, difficulty in obtaining, and easy side effects, etc., and achieve the effect of convenient operation, good biocompatibility, and simple process

Inactive Publication Date: 2012-02-15
SERICULTURAL RES INST ANHUI ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Currently the most widely used anticoagulant in clinical practice is heparin, but it is expensive, not easy to obtain, and prone to side effects when used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example example 1

[0015] Example 1: the preparation method of chitosan glycine anticoagulant derivatives, comprising the following steps:

[0016] The present invention takes by weighing 11.18g glycine and 13.88g epichlorohydrin by the molar ratio of 1:1, neutralizes glycine in the sodium hydroxide solution used in the conical flask to pH=9.0, makes sodium glycinate solution, pours into the conical flask Slowly add epichlorohydrin which is equimolar to glycine, and stir well at room temperature. At this time, the solution is an emulsion. Control the reaction in a water bath at about 50°C and continue stirring for 15 minutes until the solution turns into a light yellow clear liquid. Neutralize with hydrochloric acid to neutral. After dehydration and recrystallization with acetone, washing with 30% ethanol, evaporation to dryness with a rotary evaporator, and vacuum drying, the reaction intermediate was obtained.

[0017] Therefore weigh 2.0g chitosan, place it in a 200ml beaker, add a sodium hy...

Embodiment 2

[0018] Embodiment 2: chitosan glycine derivative of the present invention uses purification method

[0019] Since the chitosan glycine derivatives prepared by the present invention are used in the field of biomedicine and directly act on the human body, in order to avoid the mixing of harmful substances, they need to be purified to remove impurities and desalt. The present invention uses the method of dialysis to purify chitosan 1. Pass the suspension of chitosan and water through a nitrocellulose membrane, so that inorganic salts and small molecular impurities can permeate the membrane, so as to achieve the purpose of purifying chitosan.

[0020] Chitosan glycine derivatives are purified by precipitation. First, acetone is poured into the derivatives. After the derivatives are completely precipitated, the acetone is poured out, and the operation is repeated 3 times. After drying, the derivatives should be autoclaved before each use to avoid the influence of microorganisms.

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Abstract

The invention discloses a preparation method of a chitosan glycine anticoagulation derivative. The chitosan glycine derivative is obtained by preparing a sodium glycinate solution through neutralizing glycine with a sodium hydroxide solution, preparing a reaction intermediate through successively carrying out steps of a water bath reaction, neutralization to neutrality with hydrochloric acid, recrystallization, washing, evaporation to dryness with a rotary evaporator, and drying on the sodium glycinate solution and chloropropylene oxide, adding chitosan to an aqueous solution of sodium hydroxide, adding the equimolar intermediate after the complete swelling of chitosan, stirring to obtain products, dehydrating the products, washing with ethanol, and carrying out vacuum drying. The preparation method of the invention has the advantages of simple technology and convenient operation, a preparative anticoagulant (the chitosan glycine anticoagulation derivative) has an excellent anticoagulation performance, and raw materials of the anticoagulant, which have good biological compatibility, can be naturally degraded and have no toxicity and irritation, so the anticoagulant can be applied to fields of clinical biological medicines and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a preparation method of chitosan glycine anticoagulant derivatives. Background technique [0002] Heparin is currently the most widely used anticoagulant clinically, but it is expensive, not easy to obtain, and prone to side effects when used. In the medical field, there is an urgent need to develop an anticoagulant that is cheap, convenient and easy to obtain, and has a good anticoagulant effect to meet medical needs. Chitosan widely exists in the outer skin of silkworm chrysalis and other animals. It is non-toxic, non-irritating, naturally degradable, has good tissue compatibility and high biological activity, and fully meets the conditions for medical use. Moreover, there are many active groups on the chitosan molecule, which are easy to be chemically modified. [0003] Therefore, the study of chitosan-glycine derivatives has great theoretical and practical significanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61P7/02
Inventor 石凉吴大洋黄德辉孙家羿漆学文童晓琪秦凤
Owner SERICULTURAL RES INST ANHUI ACADEMY OF AGRI SCI
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