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Combination containing lutein ester and preparation method thereof

A technology containing lutein esters and lutein esters, which is applied in food preparation, food science, organic chemistry, etc., can solve problems such as safety and stability problems, hidden safety hazards, etc., to improve biological activity and ensure The effect of stability and simple process

Active Publication Date: 2012-02-15
INNOBIO CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

N-butanol is used to wash the filter cake with lower alcohols. The high boiling point of n-butanol is difficult to be completely removed when the product is vacuum-dried, which makes the product safe when used in food ingredients.
Moreover, no adsorbent was added to remove heavy metals and pesticide residues when ethyl acetate was dissolved and filtered, and no antioxidant was added to the final product, which made the safety and stability of the final product problematic.

Method used

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  • Combination containing lutein ester and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Weigh 100g of marigold extract whose total ester content is 28wt%, mix with 1100g of methyl acetate / methanol (weight ratio is 2: 8), add 7g of diatomaceous earth and filter after heating and stirring at 60°C for 3.5 hours to obtain the mother liquor I. The mother liquor I was cooled to 27° C. for 1 hour, then kept for 3 hours, then cooled to 9° C. for 1 hour, kept for 4 hours, and then filtered to obtain filter cake I.

[0034] At 55°C, add methyl acetate / methanol (weight ratio 2:8) 4 times the mass of filter cake I to dissolve filter cake I, heat and stir for 5 hours, then slowly cool down to 11°C and keep for 3.5 hours, then filter to obtain filter cake II, add 12 times the mass of methanol was stirred and washed filter cake II, 1.2g of tea polyphenols and 0.6g of vitamin C were added to the solid after washing with methanol, and dried at 45°C for 5 hours to obtain 27g of the product. The quality of the lutein-containing ester was tested. The percentage content is 67...

Embodiment 2

[0036] Take by weighing 100g total ester content and be 27wt% marigold extract and methyl acetate / ethanol (weight ratio is 2.5: 7.5) 1800g mixes, add 6.5gEDTA and 0.5g clay, filter after heating and stirring at 48 ℃ for 3 hours, obtain Mother liquor I. Slowly lower the temperature of the mother liquor I to 23.5° C. for 1 h, then keep it for 1 h, then cool it down to 13° C. for 1 h and keep it for 2.5 h, then filter to obtain the filter cake I.

[0037]At 60°C, add methyl acetate / isopropanol (5:5 by weight) of 0.5 times the mass of the filter cake I to dissolve the filter cake I, heat and stir for 2 hours, then slowly cool down to 11.5°C and keep for 4 hours, then filter to obtain the filter cake II. Add 8 times its mass of methanol and stir to wash the filter cake II, add 0.5g of vitamin E and 0.3g of vitamin C palmitate to the solid after washing with methanol, and dry it at 32°C for 17 hours to obtain 26g of the product, which is tested to contain lutein The mass percentage...

Embodiment 3

[0039] Weigh 100g of marigold extract with a total ester content of 26wt%, mix it with 1350g of ethyl acetate / methanol (weight ratio is 2:8), add 15g of clay, heat and stir at 32°C for 4.5 hours and then filter to obtain mother liquor I. Slowly lower the temperature of the mother liquor I to 26.5° C. for 1 hour, then keep it for 3 hours, then cool it down to 8.5° C. for 1 hour and keep it for 1.5 hours, then filter to obtain filter cake I.

[0040] Add ethyl acetate / isopropanol (weight ratio: 6:4) 20 times the mass of filter cake I at 65°C to dissolve filter cake I, heat and stir for 0.5h, then slowly cool down to 28°C and keep for 1h, then filter to obtain filter cake II , add 30 times the mass of isopropanol to stir and wash the filter cake II, add 1.5g of vitamin E to the solid after washing with isopropanol, and dry at 30°C for 24 hours to obtain 20g of the product. After testing the quality of lutein ester The percentage content is 70%, wherein all-trans lutein: zeaxanthi...

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Abstract

The invention relates to a combination containing lutein ester and a preparation method thereof. The preparation method comprises the steps of: decreasing the temperature of solution of a marigold extract solvent A to 20-28 DEG C, maintaining the temperature for 0.5-4 hours, decreasing the temperature to 5-18 DEG C, maintaining the temperature for 0.5-4 hours and filtering to obtain a filter cake I; and under the condition of 30-70 DEG C, dissolving the filter cake I in a mixed solvent A, agitating for 0.5-5 hours, decreasing the temperature to 10-28 DEG C, keeping the temperature for 0.5-5 hours, filtering to obtain a filter cake II and conducting subsequent steps, wherein the mixed solvent A is the mixed solvent of methyl acetate, acetic ether, n-propyl acetate or butyl acetate and C1-3 alcohol. By adopting the method provided by the invention, the process is simple and feasible, the industrialization is easy to realize, all operations are conducted at low temperature and the stability of the product is guaranteed. The yield of the obtained product highly reaches more than 25 percent, the content of total esters in the product is more than 70 percent, the content of trans-lutein highly reaches 90-93 percent and the bioactivity of the product during use is greatly improved.

Description

technical field [0001] The present invention relates to a method for isolating high lutein ester products, especially compositions with high trans-lutein ester content, from marigold extracts. In particular, it relates to a method for obtaining the above-mentioned products by direct crystallization Background technique [0002] Lutein esters are carotenoid fatty acid esters that exist in the form of fatty acids combined with lutein. It is widely found in plants such as pumpkins, spinach, cabbage, and marigold flowers. Studies have shown that the intake of lutein esters can increase the lutein levels in the blood and macula of the retina, thereby preventing age-related retinoid degeneration and cataracts, and can effectively inhibit tumor growth and prevent the occurrence of atherosclerosis. Lutein and zeaxanthin are the only carotenoids that exist in the macular area of ​​the human retina. The human body cannot synthesize lutein itself and must supplement it through daily ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L33/00
CPCC07C403/24A23L33/105
Inventor 吴文忠黄媛徐维锋范志强吕忠武刘强
Owner INNOBIO CORP LTD
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