High-efficient sterilization composition containing bupirimate
A technology of pyrimethamine sulfonate and composition, applied in the directions of fungicides, biocides, biocides, etc., to achieve the effects of excellent control effect, easy degradation and good efficacy
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example 1
[0044] (1) Experimental object:
[0045] Cucumber powdery mildew pathogen was isolated and purified from cucumber powdery mildew-infected leaves in Shandong Shouguang cucumber greenhouse, cultured and preserved on PDA slant in 4°C refrigerator.
[0046] Test medium:
[0047] PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
[0048] AEA medium: yeast powder 5g / L, glycerol 20mL / L, MgSO 4 0.25g / L, NaNO 3 6g / L, KCl 0.5g / L, KH 2 PO 4 1.5g / L, agar powder 20g / L, deionized water 1L.
[0049] (2) Experimental medicine:
[0050] 70% kasugamycin technical, Jilin Province Yanbian Chunlei Bio-Pharmaceutical Co., Ltd., 95% pyrithrimol sulfonate technical, Qingdao Hailier R&D Center Synthesis Institute.
[0051] Mix and match settings:
[0052] The content percentages of 12% pyrimifene kasugamycin are 11.5:0.5, 11:1, 10.5:1.5, 10:2, 9.5:2.5, namely 11.5%+0.5%, 11%+1%, 10.5 %+1.5%, 10%+2%, 9.5%+2.5%, a total of 5 mixing combinations.
[0053] (3) Test method:
[0054] ...
example 2
[0072] Biological Assay Example 2: Indoor Toxicity Determination of Polyoxin and Ethyrimol Sulfonate to Cucumber Downy Mildew.
[0073] Cucumber downy mildew pathogen was isolated and purified from cucumber downy mildew diseased leaves in Shouguang cucumber shed, and cultured and preserved on PDA slopes in a refrigerator at 4°C.
[0074] We performed toxicity assays for single doses of pyrithrimol and polyoxamycin, EC 50 The values were 5.76 mg / l and 4.19 mg / l, respectively.
[0075] Mix and match settings:
[0076] The content percentages of 12% pyridilsulfonate·polyoxamycin are 11:1, 10:2, 9:3, 8:4, 7:5, namely 11%+1%, 10%+2%, 9% + 3%, 8% + 4%, 7% + 5% a total of 5 mixing combinations.
[0077] The test method is similar to that of biological assay example 1.
[0078] Experimental results and analysis:
[0079] Table 3 Toxicity test of polyoxin and pyrithrimol compound against cucumber downy mildew
Virulence regression equation ...
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