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Wikstroemia indica (L.) C.A.Mey extract as well as preparation method and application thereof

A technology of extract and bromide, which is applied in the field of medicine, can solve the problems such as the lack of clear active ingredients of bromide extract, failure to give full play to bromide, and the unclear basis of medicinal effect material.

Inactive Publication Date: 2013-11-06
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Gewang is widely used clinically, but the substance basis of its efficacy is not clear. Due to the variety of active ingredients in traditional Chinese medicine, the existing technology research has not clarified the active ingredients of Gewang extract, which has failed to give full play to the good application of Gewang.

Method used

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  • Wikstroemia indica (L.) C.A.Mey extract as well as preparation method and application thereof
  • Wikstroemia indica (L.) C.A.Mey extract as well as preparation method and application thereof
  • Wikstroemia indica (L.) C.A.Mey extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of Liogewang Extract

[0040] Gewang extract was prepared as follows:

[0041] (1) After taking 1000g of the aerial part of Gewang and pulverizing it (about 1cm in length), adding 8L of ethanol with a concentration of 95% by volume and extracting at room temperature for 2 hours, the extract was concentrated and dried under reduced pressure at 40°C to obtain 80g of extract;

[0042] (2) Extract 80 g of the extract three times with 150 mL of petroleum ether, 150 mL of chloroform, 150 mL of ethyl acetate, and 150 mL of n-butanol, regardless of the order of extraction, or use one or more of them to extract Solvent extraction separately;

[0043] (3) Take the ethyl acetate extract or n-butanol extract and perform silica gel column chromatography, and elute with the gradient of chloroform:methanol (100:1-1:1, volume ratio); collect the chloroform:methanol volume ratio of 50: 1 to 1:1 elution fraction to get the effective part; the effective part c...

Embodiment 2

[0056] Example 2 Preparation of Liogewang Extract

[0057] Gewang extract was prepared as follows:

[0058] (1) Take 1000g of the above ground part of Gewang and crush it (about 1cm in length), add 8L of ethanol with a concentration of 95% by volume and extract at room temperature for 2 hours, then concentrate the extract at 40°C under reduced pressure and dry to obtain 85g of extract;

[0059] (2) Extract 80 g of the extract with 300 mL of petroleum ether, 300 mL of chloroform, 300 mL of ethyl acetate, and 300 mL of n-butanol for 4 times, regardless of the order of extraction, or use one or both of them alone The above extraction solvents are extracted respectively;

[0060] (3) Take the ethyl acetate extract or n-butanol extract and perform silica gel column chromatography, and elute with the gradient of chloroform:methanol:water (100:1:0-1:1:1, volume ratio); collect chloroform: Methanol: water volume ratio of 100:1 to 50:1 eluting components, to obtain effective par...

Embodiment 3

[0064] Example 3 Preparation of Liogewang Extract

[0065] Gewang extract was prepared as follows:

[0066] (1) Take 1000g of the above ground part of Gewang and crush it (about 1cm in length), add 8L of ethanol with a concentration of 95% by volume and extract at room temperature for 2 hours, then concentrate the extract at 40°C under reduced pressure and dry to obtain 80g of extract;

[0067] (2) Extract 80 g of the extract with 500 mL of petroleum ether, 500 mL of chloroform, 500 mL of ethyl acetate, and 500 mL of n-butanol for 4 times, regardless of the order of extraction, or use one or both of them alone The above extraction solvents are extracted respectively;

[0068] (3) Take the ethyl acetate extract or n-butanol extract and perform silica gel column chromatography, and elute with the gradient of chloroform:methanol:water (100:1:0-1:1:1, volume ratio); collect chloroform: Methanol: water volume ratio of 50:1 to 1:1 eluting components, to obtain effective parts...

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Abstract

The invention discloses a wikstroemia indica (L.) C.A.Mey extract as well as a preparation method and application thereof. The wikstroemia indica (L.) C.A.Mey extract has the effective compositions of: naringenin, 5, 6, 7- trihydroxy-4'-methoxyflavanone, p-hydroxybenzoic acid, benzoic acid, quercetin glycoside,genkwano1 B, daphnoretin, Lirioresinol, Sikokianin and the like. The invention simultaneously provides application of the wikstroemia indica (L.) C.A.Mey extract, in particular the favorable application in preparing anti-cancer or anti-inflammation analgesia drug. A cytotoxicity experiment shows that the wikstroemia indica (L.) C.A.Mey extract disclosed by the invention has cytotoxicity functions on breast cancer of human, breast cancer drugresistant cell strain, mouse breast adenocarcinoma, which are in linear relation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a Ligewang extract and its preparation method and application. Background technique [0002] Brother Wang ( Wikstroemia indlca (L.) C.A. Mey), a commonly used Chinese herbal medicine, bitter and cold in nature and taste, slightly pungent, poisonous, mainly used for bruises, dispelling blood stasis and relieving pain. The water decoction of Liaogewangye is highly sensitive to pneumococcus and staphylococcus aureus , is moderately sensitive to Pseudomonas aeruginosa and Salmonella typhi; the water decoction of rhizome bark has obvious inhibitory effect on Staphylococcus aureus in test tube; the ethyl acetate soluble part of root bark ethanol extract also has antibacterial effect in vitro , the flavonoid glycosides contained in it have inhibitory effects on Staphylococcus aureus, hemolytic streptococcus, pneumococcus and Escherichia coli in preliminary experiments; T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/20C07D311/40C07D311/30C07D311/32C07C63/06C07C65/03C07C51/42A61K31/353A61K31/352A61K31/192A61P29/00A61P35/00A61P15/14
Inventor 沈志滨尹永芹崔红花张鑫
Owner GUANGDONG PHARMA UNIV
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