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Preparation method of cordyceps militaris natto product

A soy product, the technology of Cordyceps militaris, applied in the field of microorganisms, can solve the problems that the production process of natto has not been reported yet, and achieve the effect of enhancing the immune function of the body, inhibiting cancer cells, and having a good taste

Inactive Publication Date: 2013-04-17
李光敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are hundreds of medicated recipes in China that combine the fruiting body of Cordyceps militaris with food and Chinese herbal medicines, but the production process that is compatible with natto has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Mix and dissolve 5 g of peptone, 3 g of beef extract, 5 g of NaCl, 15 g of agar and 1000 ml of water, and then adjust the pH value to 7.0 with sodium bicarbonate solution to obtain a bacterial culture medium. Put the melted bacterial culture medium into 18mm×180mm test tubes, 7ml in each test tube, stuff them with cotton plugs, sterilize at 0.14MPa for 30min, and place them on an inclined plane. After inoculating the pure Bacillus natto in the ultra-clean workbench, cultivate it at a constant temperature of 37°C until the slant is covered with bacteria to obtain the slant strain.

[0023] (2) Mix 5 g of peptone, 3 g of beef extract, 5 g of NaCl and 1000 ml of water, and then adjust the pH value to 7.0 with sodium bicarbonate solution to obtain a liquid medium. Fill 100ml of liquid medium in a 500ml glass bottle, seal it with a cotton plug, sterilize at 0.14MPa for 30min, cool to 35°C and inoculate slant strains, inoculate 0.3cm per 500ml of liquid medium 2 , and th...

Embodiment 2

[0031] (1) Mix and dissolve 5 g of peptone, 3 g of beef extract, 5 g of NaCl, 15 g of agar and 1000 ml of water, and then adjust the pH value to 7.0 with sodium bicarbonate solution to obtain a bacterial culture medium. Put the melted bacterial culture medium into 18mm×180mm test tubes, 7ml in each test tube, stuff them with cotton plugs, sterilize at 0.12MPa for 40min, and place them on an inclined plane. After inoculating the pure Bacillus natto in the ultra-clean workbench, cultivate it at a constant temperature of 37°C until the slant is covered with bacteria to obtain the slant strain.

[0032] (2) Mix 5 g of peptone, 3 g of beef extract, 5 g of NaCl and 1000 ml of water, and then adjust the pH value to 7.0 with sodium bicarbonate solution to obtain a liquid medium. Fill 100ml of liquid culture medium in a 500ml glass bottle, seal it with a cotton plug, sterilize at 0.12MPa for 40min, cool to 37°C and inoculate slant strains, inoculate 0.2cm per 500ml of liquid culture me...

Embodiment 3

[0040] (1) Mix and dissolve 5 g of peptone, 3 g of beef extract, 5 g of NaCl, 15 g of agar and 1000 ml of water, and then adjust the pH value to 7.2 with sodium bicarbonate solution to obtain a bacterial culture medium. Put the melted bacterial culture medium into 15mm×150mm test tubes, 4ml in each test tube, stuffed with cotton plugs, sterilized at 0.1MPa for 50min, and placed on a slope. After inoculating the pure Bacillus natto in the ultra-clean workbench, cultivate it at a constant temperature of 36°C until the slope is covered with bacteria to obtain the slope strain.

[0041] (2) Mix 2 g of peptone, 3 g of beef extract, 4 g of NaCl and 1000 ml of water, and then adjust the pH value to 7.2 with sodium bicarbonate solution to obtain a liquid medium. Fill 100ml of liquid medium in a 500ml glass bottle, seal it with a cotton plug, sterilize at 0.14MPa for 40min, cool to 35°C and inoculate slant strains, inoculate 0.4cm per 500ml of liquid medium 2 , and then use a reciproc...

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PUM

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Abstract

The invention discloses a preparation method of a cordyceps militaris natto product, belonging to the field of microorganism, comprising the following steps: crushing first-class soybean, wetting the crushed first-class soybean with water, and autoclaving; cooling the material, inoculating Bacillus natto liquid spawn, uniformly mixing, then keeping warm and carrying out fermentation, discharging,and drying; and crushing dried natto fermented material, then adding cordyceps militaris fruiting body powder proportionally, and uniformly mixing to obtain the cordyceps militaris natto product. Compared with pure natto powder, the prepared cordyceps militaris natto product has more stable activity of nattokinase, less peculiar smell, and better mouthfeel, and has high nutritional value and health care function.

Description

technical field [0001] The invention relates to a method for preparing a Chinese caterpillar fungus natto product, which belongs to the field of microorganisms. Background technique [0002] Cordyceps militaris and Cordyceps sinensis are heterogeneous fungi belonging to the same genus of the genus Cordyceps, and their chemical composition, nutritional composition and medicinal functions are very similar. The main medicinal ingredient of Cordyceps militaris, the content of cordycepin, etc., is much higher than that of wild Cordyceps sinensis, and it is easier to cultivate artificially than Cordyceps sinensis, so it was selected as the best substitute of Cordyceps sinensis and a large number of artificial cultivations were carried out. Artificially cultivated Cordyceps militaris is a fungal fruiting body formed by the growth of Cordyceps fungus on dead organic matter. It is usually made of rice as the main raw material, and is made through processes such as wetting, sterilizat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/29A23L33/00
Inventor 李纯端
Owner 李光敏
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