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Umbilical cord preserving fluid and preparation method thereof

A technology of preservation solution and umbilical cord, which is applied in the field of umbilical cord preservation solution, can solve the problems that the basal medium is not up to the clinical use level, the activity of stem cells is affected, and the storage time is short, so as to achieve low pollution rate, reduce accumulation, and reduce time The effect of restriction

Active Publication Date: 2012-02-01
江苏省北科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preservation solution that can reach the clinical standard and effectively preserve the umbilical cord in order to overcome the problems in the prior art that the storage time is short, the activity of the stem cells is affected, and the basal medium cannot reach the level of clinical use. The preservation solution is simple in proportion and easy to operate, so that the collected umbilical cord can effectively preserve the biological activity for a long time during the process of transportation, handover, and pre-separation detection, and improve the efficiency of material collection and use.

Method used

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  • Umbilical cord preserving fluid and preparation method thereof
  • Umbilical cord preserving fluid and preparation method thereof
  • Umbilical cord preserving fluid and preparation method thereof

Examples

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Effect test

Embodiment 1

[0019] An umbilical cord preservation solution, which contains 6000 mg of sodium chloride, 1000 mg of glucose, 10000 mg of human serum albumin, 250 mg of potassium chloride, 50 mg of anhydrous calcium chloride, 100 mg of cefoperazone sodium, and 1 mg of phenol red in 1 L of preservation solution, and the balance is injection With water, all ingredients above are injectable grade.

[0020] The preservation solution preparation method is:

[0021] (a) Take 0.5L of water for injection, add 6000mg of sodium chloride, 250mg of potassium chloride, and 50mg of anhydrous calcium chloride, and mix well;

[0022] (b) Take 0.2L of water for injection, add 1000mg of glucose and 10000mg of human serum albumin, and mix well;

[0023] (c) Take 0.1L of water for injection, add 100 mg of cefoperazone sodium and mix well;

[0024] (d) Take 0.1L of water for injection, add 1mg of phenol red and mix thoroughly;

[0025] (e) Add the solutions obtained in steps (a), (b), (c) and (d) respectively...

Embodiment 2

[0028] An umbilical cord preservation solution, which contains 6800 mg of sodium chloride, 2000 mg of glucose, 20000 mg of human serum albumin, 400 mg of potassium chloride, 300 mg of anhydrous calcium chloride, 150 mg of cefoperazone sodium, and 10 mg of phenol red in 1 L of preservation solution, and the balance is injection With water, all ingredients above are injectable grade.

[0029] The preservation solution preparation method is:

[0030] (a) Take 0.5L of water for injection, add 6800mg of sodium chloride, 400mg of potassium chloride, and 300mg of anhydrous calcium chloride, and mix well;

[0031] (b) Take 0.2L of water for injection, add 2000mg of glucose and 20000mg of human serum albumin and mix well;

[0032] (c) Take 0.1L of water for injection, add 150 mg of cefoperazone sodium and mix thoroughly;

[0033] (d) Take 0.1L of water for injection, add 10 mg of phenol red and mix thoroughly;

[0034] (e) Add the solutions obtained in steps (a), (b), (c) and (d) re...

Embodiment 3

[0037] An umbilical cord preservation solution, which contains 6500 mg of sodium chloride, 1500 mg of glucose, 15000 mg of human serum albumin, 300 mg of potassium chloride, 150 mg of anhydrous calcium chloride, 120 mg of cefoperazone sodium, and 5 mg of phenol red in 1 L of preservation solution, and the balance is injection With water, all ingredients above are injectable grade.

[0038] The preservation solution preparation method is:

[0039] (a) Take 0.5L of water for injection, add 6500mg of sodium chloride, 300mg of potassium chloride, and 150mg of anhydrous calcium chloride, and mix well;

[0040] (b) Take 0.2L of water for injection, add 1500mg of glucose and 15000mg of human serum albumin, and mix well;

[0041] (c) Take 0.1L of water for injection, add 120 mg of cefoperazone sodium and mix thoroughly;

[0042] (d) Take 0.1L of water for injection, add 5 mg of phenol red and mix well;

[0043] (e) Add the solutions obtained in steps (a), (b), (c) and (d) respectiv...

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Abstract

The invention discloses an umbilical cord preserving fluid for temporary preservation of umbilical cord after collection before separation. The umbilical cord preserving fluid comprises the following main components: sodium chloride, glucose, human serum albumin, potassium chloride and anhydrous calcium chloride, as well as auxiliary components: cefoperazone sodium and phenol red, wherein the main components have the effects on maintaining osmotic equilibrium of inner cells of the umbilical cord tissue, keeping the umbilical cord in a wet state and providing nutrient content for a short period of time; and the auxiliary components have the effects on preventing probable pollution and prompting happened pollution. The preserving fluid can be used for preserving the activity of mesenchyme stem cells in the umbilical cord, the umbilical cord can be effectively preserved at 2-10 DEG C for at least 72 hours, the activity of the separated mesenchyme stem cells is minimally influenced by time, the time limit from collection to preparation of the umbilical cord is greatly reduced, and all the used components meet a clinical use standard and are capable of effectively reducing the pollution probability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preservation solution for short-term preservation of umbilical cords from collection to separation. Background technique [0002] The umbilical cord is a tubular structure that connects the fetus and the placenta. In addition to two arteries and one vein and three blood vessels, the loose jelly-like substance (we call it Wharton's jelly) between the blood vessels and the amniotic membrane also contains rich umbilical cord mesenchyme. stem cells. Mesenchymal stem cells are an important member of the stem cell family. They have the characteristics of multi-directional differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. They are ideal seed cells for repairing tissue and organ damage caused by aging and disease. Umbilical cord mesenchymal stem cells are getting more and more attention because of thei...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 李俊杨兵成玉萍宋云庆
Owner 江苏省北科生物科技有限公司
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