Novel liposome capable of transfecting genes efficiently in vivo and in vitro and preparation method thereof

Abstract

The invention discloses a novel liposome capable of transfecting genes efficiently in vivo and in vitro and a preparation method thereof. The preparation method comprises the following steps of: weighing phosphatidylcholine and cholesterol in a molar ratio of (1-2):1, dissolving the phosphatidylcholine and the cholesterol in chloroform serving as a solvent in an amount which is 1 / 8 to 1 / 2 the mass of the phosphatidylcholine, putting the solution into a rotary flask, mixing uniformly, and sucking the solvent dry by using a vacuum aspirator to obtain a layer of lipid membrane which is attached to the wall of the flask; adding 5 mass percent glucose solution in amount which is 1 / 15 to 1 / 2 ml / g of phosphatidylcholine, dissolving the dried lipid membrane, and performing rotary drying under vacuum; and adding double distilled water until the volume of the double distilled water is equal to that of 5 mass percent glucose solution, performing ultrasonic treatment in an ultrasonic water bath tank at the temperature of 50 DEG C for 1 to 3 minutes, and collecting the liposome passing through a 0.1-micrometer polycarbonate filter membrane at the temperature of 50 DEG C to obtain the novel liposome capable of transfecting the genes efficiently in vivo and in vitro. The novel liposome capable of transfecting the genes efficiently in vivo and in vitro has the advantages of high transfection efficiency and wide transfection spectrum and particularly has the high-efficiency tumor targeting properties.

Description

Technical field: [0001] The invention belongs to the field of biomedicine, and specifically relates to a novel liposome capable of efficiently transfecting genes in vivo and in vitro and a preparation method thereof. Background technique: [0002] Gene transfection is a method of introducing foreign genes into living cells, and it is one of the most important basic links in the study of protein gene regulation, signal transduction and gene therapy. [0003] At present, the commonly used transfection methods include calcium-phosphorus transfection, electroporation, microinjection and liposome method. Calcium-phosphorus transfection method is to mix normal gene DNA (and its copy) with charged substances and calcium phosphate, DEAE-glucose or with several lipids to form precipitated DNA fine particles, which are directly poured into the medium to contact with cells Transfection method. This method is simple, but the efficiency is extremely low. Only 1%-5% of the DNA entering ...

AI Technical Summary

Problems solved by technology

At present, there are many liposome preparations in the market, but the transfection efficiency is low in some tumor cells or normal cells, and the effect in vivo is even worse, so it is difficult to apply to transfection research in animals. The target distribution of liposomes is not ideal, and the stability is poor

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