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Method for improving ginkgo leaf feeding palatability and increasing product bioactivity

A bioactive, ginkgo biloba technology, which is applied in the molding or processing of animal feed, animal feed, animal feed, etc., can solve the problems of low utilization rate of active ingredients, poor palatability, and insignificant physiological activity, etc., so as to improve feed conversion rate , reduce the content, significantly promote the growth and the effect of disease prevention and disease resistance

Active Publication Date: 2012-10-24
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007]Technical problems to be solved: The first aspect of the present invention is to overcome the poor palatability and low utilization rate of active ingredients in the direct use of ginkgo leaves and soybean meal as livestock and poultry feed or feed additives And its physiological activity is not significant and other defects, the palatability of the products prepared by the microbial fermentation treatment and the addition of flavoring agents of ginkgo leaves and soybean meal are significantly improved, and the feed intake of animals is significantly improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Selection and expansion of test bacteria: Aspergillus niger ( Aspergillus niger ) as the strain, using Zapei medium (the components of Chapei medium and their weight percentages are: glucose or sucrose 2-3%, NaNO 3 0.2%, K 2 HPO 4 .3H 2 O 0.1%, MgSO 4 .7H 2 O 0.05%, KCl 0.05%, FeSO 4 .7H 2 O 0.001%, the balance is distilled water) on the inclined plane or eggplant bottle to cultivate Aspergillus niger to obtain Aspergillus niger spores, add physiological saline to make spore suspension, the content of spores is 5×10 6 a / mL;

[0032] (2) Preparation of microbial nutrient salt solution: 1% glucose (that is, 10 g of glucose per 1 L of nutrient salt solution, the same below), NaNO 3 0.2%, K 2 HPO 4 .3H 2 O 0.1%, MgSO 4 .7H 2 The amount of O 0.05% was weighed successively and dissolved in citric acid-sodium citrate buffer solution of 50mM, pH5.0 to form a nutrient salt solution;

[0033] (3) Preparation of fermentation base material: The preparation of f...

Embodiment 2

[0038] (1) Selection and expansion of test bacteria: Aspergillus oryzae ( Aspergillus oryzae ) as the strain, using Zapei liquid medium (the components of Chapei’s medium and their weight percentages are: glucose or sucrose 2-3%, NaNO 3 0.2%, K 2 HPO 4 .3H 2 O 0.1%, MgSO 4 .7H 2 O 0.05%, KCl 0.05%, FeSO 4 .7H 2 (0.001%, the remainder is distilled water) to prepare the liquid seeds of Aspergillus oryzae, and the cultivation time is 48h;

[0039] (2) Preparation of microbial nutrient salt solution: 1.0% glucose (that is, 10 g of glucose per 1 L of nutrient salt solution, the same below), NaNO 3 0.2%, K 2 HPO 4 .3H 2 O 0.1%, MgSO 4 .7H 2 The amount of O 0.05% was weighed successively and dissolved in citric acid-sodium citrate buffer solution of 50mM, pH5.0 to form a nutrient salt solution;

[0040] (3) Preparation of fermentation base material: The fermentation material is weighed according to the weight ratio of crushed ginkgo biloba leaves: edible mushroom wa...

Embodiment 3

[0045] (1) Selection and expansion of test bacteria: Aspergillus niger ( Aspergillus niger ) as the strain, using Zapei medium (the components of Chapei medium and their weight percentages are: glucose or sucrose 2-3%, NaNO 3 0.2%, K 2 HPO 4 .3H 2 O 0.1%, MgSO 4 .7H 2 O 0.05%, KCl 0.05%, FeSO 4 .7H 2 O 0.001%, the balance is distilled water) on the inclined plane or eggplant bottle to cultivate Aspergillus niger to obtain Aspergillus niger spores, add physiological saline to make spore suspension, the content of spores is 5×10 6 a / mL;

[0046] (2) Preparation of microbial nutrient salt solution: 1.0% glucose (that is, 10 g of glucose per 1 L of nutrient salt solution, the same below), NaNO 3 0.2%, K 2 HPO 4 .3H 2 O 0.1%, MgSO 4 .7H 2 The amount of O 0.05% was weighed successively and dissolved in citric acid-sodium citrate buffer solution of 50mM, pH5.0 to form a nutrient salt solution;

[0047] (3) Preparation of fermentation base material: The fermentation...

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Abstract

A method for improving ginkgo leaf feeding palatability and increasing product bioactivity comprises the following steps: well mixing solid fermentation raw materials composed of ginkgo leaves, edible mushroom waste mushroom dregs, soybean meal and the like with a microbial nutrient salt solution, performing disinfection to obtain a solid fermentation base material; with microbe which produce beta-glucosidase as strains, performing expanding culture of the strains, inoculating the strains into the prepared solid fermentation base material for fermentation; after fermentation, drying and pulverizing the material; adding a scenting agent, well mixing to obtain the product. Application tests show that the feed additive of the invention has significant effect of growth promotion, disease prevention and disease resistance on livestock and poultry. The feed additive can significantly promote the growth of piglets and suckling pigs, has obvious effect on the control of diarrhoea diseases, can well improve the laying performance of laying hens and reduce cholesterol content of eggs, and has good prevention and adjuvant therapy effect on dairy cow mastitis.

Description

technical field [0001] The invention relates to a preparation method of a feed additive and its products, in particular to a feed additive which uses ginkgo biloba leaves, waste edible fungus residues and soybean meal as main raw materials, uses microorganisms to ferment and transform them, and compound the fermented products and flavoring agents to obtain A method for a biological feed additive and a product obtained by the method. The invention also relates to the application of the product in aquaculture, belonging to the field of feed. Background technique [0002] In order to one-sidedly pursue breeding efficiency or to prevent and resist diseases and reduce the incidence of livestock and poultry in high-density breeding, farmers will use large doses of chemically synthesized growth promoters and antibiotics. These substances will remain in livestock and poultry products, enter the human body through the food chain, and cause a series of human diseases; and the large-sc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16A23K1/14A23K10/12A23K10/30A23K10/37A23K40/00
CPCY02A40/70Y02P60/87
Inventor 赵林果曹福亮汪贵斌
Owner NANJING FORESTRY UNIV
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