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Preparation method for compound microorganism liquid state fertilizer

A technology of compound microorganisms and liquid fertilizers is applied in the field of preparation of compound microorganisms liquid fertilizers, which can solve the problems of poor sustainability of fertilizer efficiency, inconvenient collection, and environmental pollution, so as to speed up the restoration of soil fertility, reduce the existence of harmful bacteria, and accumulate more humus. Effect

Inactive Publication Date: 2012-01-11
异优坊(上海)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. Increase the application of organic fertilizers: organic fertilizers are scattered, inconvenient to collect, and expensive, and often the amount applied cannot meet the technical requirements
3. Applying bacterial fertilizer: This method is not accepted by farmers, because the fertilizer effect is not sustainable, and it is generally effective in the early stage and ineffective in the later stage
4. Green manure covers the green, and it is difficult to popularize due to the high rate of replanting by farmers and their eagerness for quick success
There are still some nutrients such as protein, amino acid, minerals and trace elements in the leftovers of hijiki still remaining in the residue as leftovers. If they are discarded, they will not only be wasted, but also pollute the environment

Method used

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  • Preparation method for compound microorganism liquid state fertilizer
  • Preparation method for compound microorganism liquid state fertilizer
  • Preparation method for compound microorganism liquid state fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Crush the hijiki leftovers to 1000-1500 mesh, soak and extract with water at 80°C for 3 hours, then concentrate the extract, add ethanol to the concentrate to make the final volume fraction of ethanol 60%, and let stand at room temperature for 5 hours , filtered to obtain the initial extract of Hijiki, the weight percentage concentration of hijiki polysaccharide in the initial extract of Hijiki is 20%, and it is separated by centrifugal partition chromatography: take 240L of chloroform, 160L of methanol, and 400L of water and place it in a mixing kettle , shake balance, stand still to separate the upper and lower phases, the upper phase is the stationary phase, and the lower phase is the mobile phase. Dissolve and filter 300 grams of hibiscus polysaccharide initial extract with 2500 ml of mobile phase solvent for later use. The stationary phase is 80 ml / min pumped in, filled the host tubular column system, turned on the centrifugal distribution chromatograph, adjusted t...

Embodiment 2

[0023] Crush the leftovers of Hijiki to 1000-1500 mesh, soak and extract with water at 80°C for 3 hours, then concentrate the extract, add ethanol to the concentrate so that the final volume fraction of ethanol is 70%, and let stand at room temperature for 5 hours , filtered to obtain the initial extract of Hijiki, the weight percent concentration of hijiki polysaccharide in the initial extract of Hijiki is 30%, and it is separated by centrifugal partition chromatography: take 240L of chloroform, 160L of methanol, and 400L of water and place it in a mixing kettle , shake balance, stand still to separate the upper and lower phases, the upper phase is the stationary phase, and the lower phase is the mobile phase. Dissolve and filter 300 grams of hibiscus polysaccharide initial extract with 2500 ml of mobile phase solvent for later use. The stationary phase is 80 ml / min pumped in, filled the host tubular column system, turned on the centrifugal distribution chromatograph, adjuste...

Embodiment 3

[0029] Crush the hijiki leftovers to 1000-1500 mesh, soak and extract with water at 80°C for 3 hours, then concentrate the extract, add ethanol to the concentrated solution so that the final volume fraction of ethanol is 80%, and stand at room temperature for 5 hours , filtered to obtain the initial extract of Hijiki; the weight percent concentration of hijiki polysaccharide in the initial extract of Hijiki is 40%, and it is separated by centrifugal partition chromatography: take 240L of chloroform, 160L of methanol, and 400L of water and place it in a mixing kettle , shake balance, stand still to separate the upper and lower phases, the upper phase is the stationary phase, and the lower phase is the mobile phase. Dissolve and filter 300 grams of hibiscus polysaccharide initial extract with 2500 ml of mobile phase solvent for later use. The stationary phase is 80 ml / min pumped in, filled the host tubular column system, turned on the centrifugal distribution chromatograph, adju...

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Abstract

The invention relates to a preparation method for compound microorganism liquid state fertilizer, which is characterized by comprising the following steps: crushing leftovers of Sargassum fusiforme to 1000-1500 meshes, digesting and extracting by water, then concentrating an extract, adding ethanol in a concentrate until the final volume fraction of ethanol to be 60-80%, standing for 5 hours at the room temperature, filtering to obtain an original extract of the Sargassum fusiforme, wherein the concentration of weight percentage of Sargassum fusiforme polysaccharide of the original extract is 20-40%, separating by using a centrifugal chromatography to obtain Sargassum fusiforme polysaccharide; adding enzyme into Sargassum fusiforme polysaccharide for hydrolyzing, centrifugating, ultrafiltrating to obtain a nutrient solution; placing the nutrient solution into a cage-type ventilation stirring reactor for microorganism continuous fermentation; mixing fermented products and negative potential energy water to obtain the compound microorganism liquid state fertilizer. The invention is capable of effectively repairing soil, gradually eliminating the phenomena of soil hardening, acidifying and salting of soil, increasing the beneficial bacteria in soil and reducing the existence of pernicious bacteria.

Description

technical field [0001] The invention relates to a preparation method of a compound microbial liquid fertilizer and belongs to the technical field of microbial fertilizers. Background technique [0002] Decades of bad farming in our country have led to nutrient loss and the quality of cultivated land has been seriously damaged. According to the data analysis of 200 soil nutrient testing points in China, the national soil organic matter content is low, with an average of 1.8%, most of which are less than 1%. In the past ten years, 70-80% of the cultivated land in my country has insufficient nutrients, and 20-30% of the cultivated land has over-nutrient. Long-term use of inorganic fertilizers will cause soil compaction, acidification, and salinization, causing environmental pollution. [0003] At present, developed countries in Europe and the United States mainly adopt the following measures to protect cultivated land: land fallow, no-tillage or less tillage; green manure cove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05F11/08C05G3/80
Inventor 黄顺勇马永明李平英
Owner 异优坊(上海)生物科技有限公司
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