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Dentoidin sponginum and preparation method thereof

A tooth matrix and sponge technology, applied in the medical field, to promote the differentiation of osteoblasts and chondrocytes, enhance plasticity, and increase the dosage of bone matrix

Active Publication Date: 2013-04-17
深圳市光明创博生物制品发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scholars tried to use genetic engineering and other methods to synthesize a large amount of BMP and use it for bone repair. They found that although it has the effect of improving osteogenesis, it is easy to diffuse in the body and be degraded by proteases. The carrier controls the slow release to make it work for a longer time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of Tooth Matrix

[0033] (1) Soak the raw material of mammalian raw teeth with 50% sodium hypochlorite disinfectant heated to 40°C and stir and digest for 30 minutes to remove soft tissue and disinfect;

[0034] (2) Remove enamel, cementum, etc. from the sterilized mammalian original tooth raw materials by mechanical means, and retain the dentin;

[0035] (3) Mechanically pulverize dentin to make it into 10-100 mesh particles;

[0036] (4) Remove the soft tissue and disinfect the pulverized granular dentin again according to step (1);

[0037] (5) Put into 1:1 chloroform formaldehyde of 5 times of volumes and soak for 30 minutes;

[0038] (6) After rinsing with clean running water for 4 hours, soak in 5 times the volume of 0.6M HCl for 18 hours;

[0039] (7) The tooth matrix was obtained after repeated rinsing with sterile deionized water.

Embodiment 2

[0040] Example 2: Preparation of Tooth Matrix

[0041] (1) Soak the raw material of mammalian raw teeth with 50% sodium hypochlorite disinfectant heated to 40°C and stir and digest for 45 minutes to remove soft tissue and disinfect;

[0042] (2) Mechanically remove enamel, cementum and decayed teeth from the sterilized mammalian raw materials, and retain the dentin;

[0043] (3) Mechanically pulverize dentin to make it into 10-100 mesh particles;

[0044] (4) Remove the soft tissue and disinfect the pulverized granular dentin again according to step (1);

[0045] (5) Put into 1:1 chloroform formaldehyde of 5 times of volumes and soak for 30 minutes;

[0046] (6) After rinsing with clean running water for 4 hours, soak in 5 times the volume of 0.6M HCl for 24 hours;

[0047] (7) The tooth matrix was obtained after repeated rinsing with sterile deionized water.

Embodiment 3

[0048] Example 3: Preparation of Tooth Matrix

[0049] (1) Soak the raw material of mammalian raw teeth with 50% sodium hypochlorite disinfectant heated to 40°C and stir and digest for 45 minutes to remove soft tissue and disinfect;

[0050] (2) Mechanically remove enamel, cementum and decayed teeth from the sterilized mammalian raw materials, and retain the dentin;

[0051] (3) Mechanically pulverize dentin to make it into 10-100 mesh particles;

[0052] (4) Remove the soft tissue and disinfect the pulverized granular dentin again according to step (1);

[0053] (5) Put into 1:1 chloroform formaldehyde of 5 times of volumes and soak for 30 minutes;

[0054] (6) After rinsing in clean running water for 4 hours, soak in 5 times the volume of 0.65M HCl for 24 hours;

[0055] (7) The tooth matrix was obtained after repeated rinsing with sterile deionized water.

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PUM

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Abstract

The invention belongs to the field of pharmacy and medical technology and relates to a dentoidin sponginum and a preparation method thereof. The sponginum provided by the invention comprises 80-99wt% of dentoidin and 1-20wt% of collage sponge matrix, wherein the dentoidin is fixed on the collagen sponge through crosslinking in different weight ratios. The sponginum provided by the invention ensures that the dentoidin is filled into ill parts with bone defects, nonunion of bone, bone tissue atrophy, bone fracture, poor bone healing and the like, so that the dentoidin plays roles in inducing bone formation, promoting bone repair and promoting wound convergence.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a dental matrix sponge and a preparation method. The sponge can shape the tooth matrix very well, so that the tooth matrix can be better filled in bone defect, nonunion, bone tissue atrophy, fracture, poor bone union and other diseased parts, and it can be used continuously for a long time with an effective dose. It induces osteogenesis, promotes bone repair, and promotes wound convergence. Background technique [0002] Dr. Urist from the United States discovered that decalcified bone matrix can induce the formation of bone and cartilage, and in 1965 he established the theory of osteoinductive repair centered on the induction of bone tissue formation by BMP in bone. Since then, scholars at home and abroad have conducted a lot of research on the theory of bone repair, and developed a series of osteogenic materials derived from organisms, mainly autologous bone materials and frozen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/32A61K38/39A61K9/00A61K47/42A61K47/48A61P19/08
Inventor 李佳楠
Owner 深圳市光明创博生物制品发展有限公司
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