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A method to enhance the immunogenicity of small molecule organics

A technology of immunogenicity and organic matter, applied in the field of immunology, can solve the problems of masking, inability to effectively enhance the immunogenicity of target small molecule organic matter, and destruction of antigenic determinants, and achieve low cost, enhanced immunogenicity, and easy preparation Effect

Active Publication Date: 2011-12-28
苏州良辰生物医药科技有限公司
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Problems solved by technology

However, in practice, in many cases, the use of EDC and SSMCC as cross-linkers cannot effectively enhance the immunogenicity of the target small molecule organics, which may be due to the destruction or masking of the epitopes of the small molecule organics during the modification process

Method used

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Embodiment Construction

[0015] The technical solutions of the present invention will be further described below in conjunction with specific examples, but the present invention should not be limited to these examples.

[0016] A method for enhancing the immunogenicity of 2,3Dinor TxB2, the specific steps are as follows:

[0017] (1) Add 5mg of 2,3Dinor TxB2 (1ml) and 10mg (1ml) of albumin into a 15ml test tube.

[0018] (2) Add 20 mg of a cross-linker (1 ml), which is composed of PEG with a molar mass of 4000 kDa and succinimide succinate modified at both ends.

[0019] (3) Act for 2 hours at room temperature to combine the cross linker with 2,3Dinor TxB2;

[0020] (4) dialyze with PBS buffer solution for 2 hours;

[0021] (5) Adjust the concentration of the conjugate of 2,3Dinor TxB2 and the cross-linker to about 500 μg / ml. 200 microliters are dispensed in small plastic bottles.

[0022] (6) Specific polyclonal antibodies are prepared by immunizing animals with coupled antigens and adding approp...

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Abstract

The invention relates to a method for enhancing immunogenicity of a micromolecular organic matter. The method is characterized in that a cross connector is used to connect the micromolecular organic matter with a protein vector, especially, the cross connector is formed by modifying active groups at the two ends of a linear polymer, wherein the molar mass of the linear polymer is 500-10000kDa. According to the invention, the connector with a special structure is adopted; and after the micromolecular organic matter is connected with the protein vector by using the connector, the antigenic determinant of the micromolecular organic matter is not easy to cover, thus the immunogenicity of the micromolecular organic matter is obviously enhanced. In addition, the preparation of the connector is extremely easy, and the method is easy and is low in cost.

Description

technical field [0001] The invention relates to the field of immunology, in particular to a method capable of enhancing the immunogenicity of small molecular organic substances. Background technique [0002] Antigen is a kind of substance that can stimulate the body's immune system to produce a specific immune response, and can specifically bind to the corresponding immune response product in vivo or in vitro. Antigen has two characteristics: ① Immunogenicity (immunogenicity) means that antigen can stimulate specific immune cells, activate immune cells, proliferate, differentiate, and finally produce immune effector substances (antibodies and sensitized lymphocytes). ②Immune reactivity (immunoreactivity) refers to the characteristic that when an antigen meets the corresponding immune effector substances (antibodies and sensitized lymphocytes), specific binding can occur to generate an immune response (also known as reactogenicity). Substances with these two properties are c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K17/08
Inventor 王小良
Owner 苏州良辰生物医药科技有限公司
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