A method and kit for detecting amylase

Abstract

The invention relates to the technical field of medical detection and discloses a method and a kit for detecting amylase. The method for detecting the amylase provided by the invention comprises the following steps of: in a buffer solution with a pH value of 7.2, mixing a sample to be detected and soluble starch to obtain a mixed solution; mixing potassium iodide and acidic potassium iodate and mixing the mixture of the potassium iodide and the acidic potassium iodate with the obtained mixed solution; detecting absorbance in a 630nm wavelength; and calculating the concentration of the amylase in the sample to be detected according to the absorbance of a non-enzymatic blank tube which is subjected to the same treatment. In the method, the potassium iodide and the potassium iodate generate iodine under the acidic condition, and the iodine is combined with the unreacted starch to form a blue compound, so influence on reaction color development due to reduction of components caused by easiness in sublimation and volatilization of an iodine solution which is directly used is avoided, and the accuracy of a detection result is improved. The kit is good in stability, long in preservation time, wide in application range and convenient for popularization and can be applied to determination of the content of the amylase in serum or urine by hospitals, health departments and medical biological scientific research institutes at all levels.

Description

technical field [0001] The invention relates to the technical field of medical testing and determination, in particular to a method and kit for detecting amylase. Background technique [0002] Amylase (α-Amylase), referred to as AMS or AMY, is a type of enzyme that can hydrolyze starch molecules. It generally acts on α-1,4-glucans such as soluble starch, amylose, and glycogen to hydrolyze α-1, 4-Glycosidic bonds, resulting in dextran, maltose and glucose molecules. Amylase is abundant in the pancreas and salivary glands. Pancreatic amylase is excreted into the digestive tract in an active state by the pancreas. It is the most important enzyme that hydrolyzes carbohydrates. It belongs to α-amylase like the amylase secreted by salivary glands. Amylase has a small molecular weight and can be filtered through the glomerulus and excreted with urine. When suffering from pancreatic diseases or pancreatic exocrine dysfunction, amylase activity can be increased or decreased. Theref...

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Problems solved by technology

[0005] However, the chromogenic agent iodine solution in the above method is easy to sublimate and volatilize, causing the components to decrease and become lighter, which affects the color development of the reaction, resulting in a decrease in the accuracy of the test results.

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