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Application of Paeonol in Radiosensitization of Malignant Tumors

A technology for radiosensitization and malignant tumors, which is applied in the field of new medical applications of paeonol as a radiosensitizer, which can solve the problems of high toxicity and achieve the effect of increasing the radiosensitivity of tumor cells

Inactive Publication Date: 2011-12-21
孙国平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some radiation sensitizers have transitioned to clinical trials after the completion of preliminary trials. Most of the drugs were eventually abandoned due to excessive toxicity or failure to achieve the expected effect. not much

Method used

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  • Application of Paeonol in Radiosensitization of Malignant Tumors
  • Application of Paeonol in Radiosensitization of Malignant Tumors
  • Application of Paeonol in Radiosensitization of Malignant Tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Effect of paeonol on the radiosensitivity of Lewis lung cancer cells in mice

[0021] 【Method】The colony formation experiment was used to measure the survival rate of cells under different intervention conditions, and the cell survival curve was fitted by a single-click multi-target model, and the relevant parameters and radiosensitivity ratio (SER) were calculated. For specific methods, please refer to references: Yin Weibo, Yu Zihao, Xu Guozhen, etc., Radiation Therapy for Tumors (Fourth Edition) [M], Peking Union Medical College Press, 2007: 234-237

[0022] [Results] See the attached results figure 1 and Table 1.

[0023] Table 1: The main parameter values ​​of the survival curve

[0024]

[0025] The test results showed that when 5.0 and 10.0 mg / LPae were combined with radiation, the average lethal dose D0 and the quasi-threshold dose Dq both decreased, and the radiosensitization ratios SER were 1.081 and 1.164, respectively; , increased cellular r...

Embodiment 2

[0026] Example 2: The radiosensitization effect of paeonol on the mouse Lewis lung cancer xenograft model

[0027] 【method】

[0028] (1) Production of animal models: 72 C57BL / 6 mice, male, 6-8 weeks old, 20±2 grams. Prepare Lewis lung cancer cell suspension at a concentration of 1×10 7 individual / mL. 0.2 mL of cell suspension was subcutaneously injected into the right lower limb of each mouse. After 5-7 days, the experiment was started when the tumor volume increased to 800±100mm3.

[0029] (2) Grouping: 72 mice were randomly divided into 6 groups (12 in each group): model group, low-dose drug group, high-dose drug group, radiation group, low-dose drug+radiation group, high-dose drug+radiation group .

[0030] (3) Intervention conditions: irradiation dose: single 10Gy; low dose drug: 20% LD50 (156.2 mg / kg) intraperitoneal injection; high dose drug: 50% LD50 (390.5 mg / kg) intraperitoneal injection.

[0031] (4) Observation indicators: tumor volume, growth time, growth del...

Embodiment 3

[0036] Example 3: Effect of paeonol on radiosensitivity of lung adenocarcinoma A-549 cells

[0037] 【Method】The colony formation experiment was used to measure the survival rate of cells under different intervention conditions, and the cell survival curve was fitted by a single-click multi-target model, and the relevant parameters and radiosensitivity ratio (SER) were calculated. For specific methods, please refer to references: Yin Weibo, Yu Zihao, Xu Guozhen, etc., Radiation Therapy for Tumors (Fourth Edition) [M], Peking Union Medical College Press, 2007: 234-237

[0038] [Result] see the result image 3 and Table 3.

[0039] Table 3: Effect of paeonol on radiosensitivity of lung adenocarcinoma A549 cells (cell survival curve parameters)

[0040]

[0041] The test results illustrate that with 20% IC 50 Three concentrations of paeonol (2.5, 5.0, 10.0 mg / L) selected on the left and right can increase the radiosensitivity of A-549 cells after 24 hours in a dose-dependent...

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Abstract

The present invention relates to the new medical use of paeonol, in particular to the new medical use of paeonol as a radiation sensitizer. Surprisingly, the present invention finds that paeonol has the effect of increasing the radiosensitivity of tumor cells, has obvious in vivo and external radiosensitization effects on Lewis lung cancer, and has the effect on lung A-549 cells, glioma C6 cells, and esophageal cancer cells. ECA-109 cells have broad-spectrum radiation sensitization. Compared with the existing radiosensitizers, paeonol has the characteristics of high efficiency and low toxicity, and it is expected to become a safe and effective new drug for radiosensitization.

Description

technical field [0001] The present invention relates to the new medical use of paeonol, in particular to the new medical use of paeonol as a radiation sensitizer. It belongs to the field of medical technology. Background technique [0002] Although the technology of current radiotherapy has made great progress, the effect of radiotherapy is still not ideal. An important factor affecting the effect of radiotherapy is the radioresistance of tumor cells, and improving the sensitivity of tumor cells to radiation by radiosensitizers is a current research hotspot. Radiosensitizers are a class of chemically synthesized or naturally extracted pharmaceutical preparations that can effectively increase the radiosensitivity of tumor cells and promote the killing effect of radiation on tumor cells. [0003] Human hypoxic cells have obvious resistance to radiation, which is the reason for the failure of various tumor radiotherapy or even recurrence and metastasis. Early research on radi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P35/00A61P35/02A61P35/04
Inventor 孙国平雷宇
Owner 孙国平
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