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Application of Cordyceps diffusa in Controlling Tobacco Bacterial Wilt

A technology for Cordyceps diffusa and tobacco bacterial wilt, which is applied in the direction of application, chemicals for biological control, biocides, etc., can solve the problems that have not been reported in research and application, and achieve good social and ecological benefits, Simple process and good control effect

Inactive Publication Date: 2011-12-21
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The germplasm resources of G. diffusa are abundant, and they are widely distributed in Hainan, Guangdong, Guangxi, Taiwan and other provinces in my country. At present, the research and application of its extracts in the prevention and treatment of plant diseases and as green manure have not been reported.

Method used

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  • Application of Cordyceps diffusa in Controlling Tobacco Bacterial Wilt
  • Application of Cordyceps diffusa in Controlling Tobacco Bacterial Wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Extraction of the Active Substances of Glycyrrhiza diffusa

[0016] Collect 240.0kg of the whole herb of Diphtheria radix, wash it with water, dry it in the sun, and crush it. Half of the raw material is soaked in ethanol for 7 days, and extracted by cold soaking. It is extracted 3 times in total, each time for 5d to 7d, filtered and combined to concentrate the filtrate into In the form of a paste, 1453.0 g of the ethanol extract of Echinopsis diffusa was obtained. The other half was boiled with water and extracted 3 times, each extraction was 1h-3h, filtered and the filtrate was concentrated into a paste to obtain 1643.0g of the water extract of Echinacea diffusa.

Embodiment 2

[0017] Example 2 Anti-tobacco Ralstonia solanacear activity test of active ingredients

[0018] Using the active substance of G. diffusa in Example 1, the ethanol extract and the water extract were numbered 1 and 2 respectively, and the organic solvent chloroform was added to prepare a 1mg / 20μL sample solution, and each 1.6μl was taken to measure its antibacterial Activity of the tobacco pathogen R. solanacearum. As the positive control, carbendazim with a total active ingredient content of 50% produced by Shandong Haixun Biochemical Co., Ltd. was selected.

[0019] Tobacco solanacear pathogen is activated with beef extract solid medium, and its liquid medium is used to cultivate pathogenic bacteria for subsequent use (concentration is about 1 × 10 7 ). Cool the freshly sterilized solid beef extract culture medium to 50-60°C (not hot), add 2000 μL tobacco solanacearum culture solution to each 200mL medium, shake well, and pour it into a plate for later use. After the plate ...

Embodiment 3

[0023] Example 3 Field Test of Active Substances of Glycyrrhiza diffusa against Tobacco Ralstonia Solanacearum

[0024] A control field and a blank control field were set up, and the control field was separately applied with the ethanol extract and water extract produced in Example 1, which were added to ethanol to prepare a 1g / 20L fungicide. The blank control field was not treated, and repeated 4 times.

[0025] The experiment was arranged in random blocks, 20 tobacco plants were planted in each plot, and protective rows were set around. The fungicides in Example 3 were used in the control field respectively, and the roots were diluted 1 hour before the application. The application time was: from the beginning of the disease, once every 10 days, and twice, 30-40ml per plant. The incidence rate and disease index of each tobacco plant were investigated before each root irrigation, and the surveyed data were statistically analyzed to compare the differences in the inhibition of...

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Abstract

The invention relates to the agricultural application of Echinopsis diffusa. Both the ethanol extract and water extract of the whole herb of G. radix had good antagonistic activity against the pathogen of R. solanacearum. The extract is a natural plant source component, which can replace chemical pesticides, reduce environmental pollution and residues of chemical pesticides, and is an ideal biological fungicide in agriculture. The use of the whole plant as green manure has good control effect on the occurrence of tobacco bacterial wilt and can promote the growth of tobacco plants, which has good economic, social and ecological benefits.

Description

technical field [0001] The invention relates to a method for extracting plant active ingredients and its application in plant diseases, in particular to the application of Echinopsis diffusa in preventing and treating tobacco bacterial wilt. Background technique [0002] Elephantopus tomentosus Linn., also known as Bugloss (Hainan Island), belongs to the genus Elephantopus in the Compositae family. There are more than 30 species in this genus, most of which are produced in America, and a few Distributed in Africa, Asia and Oceania. In my country, there are only two species of Echinacea and Echinacea diffusa, which are distributed in South China and Southwest China, and grow in hillside wilderness, roadside or bushes. [0003] Glycyrrhiza radix is ​​the botanical source of the traditional Chinese medicine gall gall. Folks believe that gallbladder has the effects of clearing away heat, cooling blood and promoting dampness. As early as in the "Lingnan Herbal Medicine", it wa...

Claims

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Application Information

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IPC IPC(8): A01N65/12A01P1/00C05G3/00A01G1/00A01C21/00
Inventor 戴好富莫明河刘国道梅文莉王蓓王辉
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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