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Method for quickly reproducing bletilla striata seedlings

A Bletilla striata seedling and rapid technology are applied in the field of rapid propagation of Bletilla striata seedlings, which can solve the problems of extreme shortage of supply in the market, quality degradation, low effective yield and the like, and achieve the effect of alleviating the contradiction between supply and demand in the market and increasing the output.

Active Publication Date: 2013-07-24
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the artificial cultivation of asexually propagated seedlings carrying viruses, quality degradation, and low effective yield, the market is extremely short of supply, and prices have been rising year after year. Solving the breeding technology of Bletilla striata is the key to alleviating the contradiction between supply and demand in the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Disinfection of explants: first rinse the capsules with running water, rinse with washing powder solution, wash with distilled water, soak in 75% alcohol for 28 seconds, then soak in 0.15% mercury liter solution for 12 minutes, finally rinse with sterile water 3 times, and set aside.

[0021] 2) Protocorm formation and proliferation: evenly shake off the sterilized seeds into sterile water or germination medium, which is 1 / 2 MS solid medium with 1.05 mg / Kg 6-benzylaminopurine added. After about 7 days, the seed embryo starts to expand, and the protocorm is formed in about 14 days. After the protocorm is cut, it is transferred to the protocorm proliferation medium to obtain a large number of protocorms. Wherein the protocorm proliferation medium is 1 / 2MS solid medium supplemented with 1.08mg / Kg 6-benzylaminopurine, 0.95mg / Kg naphthaleneacetic acid, 28g / Kg sucrose and 4.6g / Kg agar. The culture conditions are: temperature 24°C, relative humidity 70%, light time 12 hours...

Embodiment 2

[0026] 1) Disinfection of explants: first rinse the capsules with running water, rinse with washing powder solution, wash with distilled water, soak in 75% alcohol for 25 seconds, then soak in 0.1% mercury liter solution for 10 minutes, and finally rinse with sterile water twice, and set aside.

[0027] 2) Protocorm formation and proliferation: Shake the sterilized seeds evenly into sterile water or germination medium. After about 7 days, the embryos start to expand, and form a protocorm after about 14 days. Cut the protocorm and transfer it to the protocorm for proliferation culture medium to obtain a large number of protocorms. Wherein the protocorm proliferation medium is 1 / 2MS solid medium with 0.9mg / Kg 6-benzylaminopurine, 0.08mg / Kg naphthaleneacetic acid, 25g / Kg sucrose and 4g / Kg agar; the culture conditions are: temperature 23°C, The relative humidity is 65%, the light time is 11 hours / day, and the light intensity is 2000Lx.

[0028] 3) Cluster bud induction and prolif...

Embodiment 3

[0032] 1) Disinfection of explants: first rinse the capsules with running water, rinse with washing powder solution, wash with distilled water, soak in 75% alcohol for 35 seconds, then soak in 0.2% mercuric solution for 15 minutes, finally rinse with sterile water 5 times, and set aside.

[0033] 2) Protocorm formation and proliferation: Shake the sterilized seeds evenly into sterile water or germination medium. After about 7 days, the embryos start to expand, and form a protocorm after about 14 days. Cut the protocorm and transfer it to the protocorm for proliferation culture medium to obtain a large number of protocorms. Among them, the protocorm proliferation medium is 1 / 2MS solid medium with 1.2mg / Kg 6-benzylaminopurine, 0.12mg / Kg naphthaleneacetic acid, 35g / Kg sucrose and 6g / Kg agar; the culture conditions are: temperature 26°C, The relative humidity is 80%, the light time is 14 hours / day, and the light intensity is 5000Lx.

[0034]3) Cluster bud induction and proliferat...

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Abstract

The invention provides a method for quickly reproducing bletilla striata seedlings, which comprises the following steps: 1) using mature seeds of bletilla striata as explants; 2) sterilizing the seeds and inoculating the seeds to a germination culture medium to allow the seeds to germinate in about 7 days, cutting protocorms formed in about 14 days, and transferring the cut protocorms onto a protocorm proliferation culture medium; 3) transferring the protocorms onto a cluster bud induction and proliferation culture medium; and 4) cutting the cluster buds, allowing the cluster buds to propagate massively, transplanting 3-centimeter seedlings grown from the cluster buds onto a rooting and seedling strengthening culture medium, and transplanting the seedlings when the seedlings grow to 7 to 10 centimeter. The method solves the problems of virus carrying seedlings, deterioration of variety, low effective yield and the like of the conventional bletilla striata asexual reproduction. When the method is used, a large number of asexual reproduction virus-free seedlings can be obtained under a manual control condition; compared with the conventional reproduction method, the yield is improved by 30 percent; and thus, the method has an active significance for reliving market contradiction between supply and demand for bletilla striata.

Description

technical field [0001] The invention belongs to the technical field of rapid plant propagation, and in particular relates to a method for rapid propagation of bletilla striata seedlings. Background technique [0002] Bletilla striata (Bletilla striata (Thunb.) Reichb.f.) is a perennial herbaceous plant of the Orchidaceae (Orchidaceae) plant Bletilla striata, which is used as medicine with dry tubers. It has the effects of astringent and hemostasis, detumescence and granulation promoting. It has a good curative effect on hemoptysis, hematemesis, blood in the stool and trauma hemostasis, sore swelling, cracked hands and feet, etc. It is widely used in the treatment of pulmonary tuberculosis, bronchiectasis hemoptysis, gastric ulcer bleeding and other diseases. Its bletilla striata glue is widely used in food, medicine and Daily chemicals and other fields. In recent years, wild bletilla striata has been over-harvested, resulting in endangered wild resources. Due to the artif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 齐香君王钏施春阳
Owner SHAANXI UNIV OF SCI & TECH
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