PRRS rt-lamp detection method and detection kit
A technology for detection kits and detection methods, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of cumbersome operation, difficult diagnosis, long quarantine period, etc., and achieve high sensitivity, high specificity, very good effect
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[0035] The specific construction steps of the present invention are further elaborated below, mainly including the following steps:
[0036] 1. Design and synthesis of RT-LAMP primers:
[0037] Primer design is the core of establishing the LAMP method. It has certain similarities with PCR primers, such as the optimal GC content of primers is 40% to 60%, and the requirement to avoid forming a complementary hairpin structure at the 3' end as much as possible, but there are also differences. , The following principles should be followed when designing LAMP primers: the distance between the 5' end of the F2 primer and the 5' end of the B2 primer should preferably be controlled within 120 to 180 bp, between the F2 primer and the F3 primer, and between the B2 primer and the B3 primer The distance should be within 20bp; the Tm value of the primer is 60 to 65°C for normal or GC-rich regions, and 55 to 60°C for AT-rich regions. At the same time, the Tm value of the primers should meet ...
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