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Fusion protein of active group of hepatocyte growth factor receptor

A technology of hepatocyte growth factor and active group, applied in the field of DNA, can solve the problem of no obvious improvement in lung cancer patients, and achieve the effect of reducing autoimmune reactions

Active Publication Date: 2011-12-14
江苏宁录科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, chemotherapy does not significantly improve the majority of lung cancer patients

Method used

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  • Fusion protein of active group of hepatocyte growth factor receptor
  • Fusion protein of active group of hepatocyte growth factor receptor
  • Fusion protein of active group of hepatocyte growth factor receptor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (Example 1, Gene Sequence and Preparation of SMI-Fc Fusion Protein)

[0041] The structure of the SMI-Fc fusion protein constructed in this example is shown in figure 2 (a). The SMI-Fc fusion protein is fused with human immunoglobulin hIgG1-Fc by a functional group composed of Sema protein active group, MRS protein active group and IPT protein active group connected end to end.

[0042] The SMI-Fc fusion protein gene is obtained by amplifying the gene fragments of the above-mentioned Sema protein active group, MRS protein active group, IPT protein active group and hIgG1-Fc through polymerase chain reaction (PCR).

[0043] When performing PCR amplification, PCR primers cMet1F and cMet2RL and cMet nucleic acid template (nucleotide sequence sees SEQ ID NO.14), PCR primers cMet2FL and hIgGFcRev1 and human immunoglobulin heavy chain nucleic acid template (nucleotide sequence sees SEQ ID NO.14) NO.11) First perform the first PCR amplification respectively to obtain two PCR...

Embodiment 2

[0051] (Example 2, Gene Sequence and Preparation of SM-Fc Fusion Protein)

[0052] The structure of the SM-Fc fusion protein constructed in this example is shown in figure 2 (b). The SM-Fc fusion protein is fused with human immunoglobulin hIgG1-Fc by combining the functional groups formed by the Sema protein active group of cMet and the MRS protein active group end-to-end.

[0053] The SM-Fc fusion protein gene is obtained by amplifying the gene fragments of the above-mentioned Sema protein active group, MRS protein active group and hIgG1-Fc through polymerase chain reaction (PCR).

[0054] When performing PCR amplification, PCR primers cMet1F and cMet3RL and cMet nucleic acid template (nucleotide sequence sees SEQ ID NO.14), PCR primers cMet3FL and hIgGFcRev1 and human immunoglobulin heavy chain nucleic acid template (nucleotide sequence sees SEQ ID NO.14) NO.11) Perform the first PCR amplification respectively to obtain two PCR products; then PCR primers cMet1F and hIgGFc...

Embodiment 3

[0058] (embodiment 3, SM 2 - Preparation of Fc fusion protein)

[0059] The SM constructed in this example 2 -Fc fusion protein structure see figure 2 (c). SM 2 - The Fc fusion protein is fused with human immunoglobulin hIgG1-Fc by fusing two groups of Sema protein active groups and MRS protein active groups of cMet connected end-to-end.

[0060] SM 2 - The Fc fusion protein gene is obtained by amplifying the gene fragments of the above-mentioned Sema protein active group, MRS protein active group and hIgG1-Fc through polymerase chain reaction (PCR).

[0061] When performing PCR amplification, PCR primers cMet1F and cMet4RL and cMet nucleic acid template (see SEQ ID NO.14 for the nucleotide sequence), PCR primers cMet4FL and cMet3RL and cMet nucleic acid template (see SEQ ID NO.14 for the nucleotide sequence), The PCR primers cMet3FL and hIgGFcRev1 and the human immunoglobulin heavy chain nucleic acid template (see SEQ ID NO.11 for the nucleotide sequence) were firstly ...

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Abstract

The invention discloses a fusion protein of a hepatocyte growth factor receptor active group, and the fusion protein expresses various fusion proteins of single or more hepatocyte growth factor receptor active groups and human immunoglobulin Fc segments in different combinations by utilizing a molecular biology technology. The fusion protein of the hepatocyte growth factor receptor active group can be efficiently and specifically combined with a growth factor, prevents the growth factor from combining with tumor cells, inhibits growth and transfer of the tumor cells, and treats tumor in a target localization way; and due to the diversity of the hepatocyte growth factor receptor active group, the fusion protein with different affinities and pharmacokinetics can be obtained by virtue of the combination of single or more receptor active groups.

Description

technical field [0001] The invention relates to a fusion protein, in particular to a fusion protein encoding a hepatocyte growth factor receptor (cMet) active group and an Fc fragment and its encoding DNA. Background technique [0002] Hepatocyte growth factor (hepatocyte growth factor, HGF), also known as scatter factor (SF), is a heterodimer composed of an α heavy chain (relative molecular weight of 69 kDa) and a β light chain (relative molecular weight of 34 kDa). Polymer. HGF is a mitogen that stimulates hepatocyte proliferation and plays an important role in hepatocyte regeneration after hepatectomy or chemical injury. HGF is a multifunctional cytokine that can stimulate the differentiation, proliferation, regeneration, movement and morphological changes of various types of cells. [0003] The receptor of HGF is the hepatocyte growth factor receptor (cMet), which is a proto-oncogene (Proto-Oncogen) cloned from NIH3T3 cells by Cooper et al. in 1984. The mutation can be...

Claims

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Application Information

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IPC IPC(8): C07K19/00A61K38/17A61K39/395A61P35/00
Inventor 周斌
Owner 江苏宁录科技股份有限公司
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