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A method for continuous separation of cyclic adenosine monophosphate

A technology of cyclic adenosine monophosphate and continuous ions, which is applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve the problems of high cost, difficult scale, cumbersome operation, etc., and achieve operating cost saving and reduction The effect of simple resin dosage and process steps

Inactive Publication Date: 2011-12-07
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Therefore, the purpose of the present invention is to overcome the shortcomings of the current separation process of cyclic adenosine monophosphate, such as high cost, cumbersome operation, and difficulty in scale-up, and provide a method for continuously separating cyclic adenosine monophosphate with low cost, easy operation, and large-scale application

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  • A method for continuous separation of cyclic adenosine monophosphate
  • A method for continuous separation of cyclic adenosine monophosphate
  • A method for continuous separation of cyclic adenosine monophosphate

Examples

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Embodiment 1

[0043] This example is used to illustrate the method for continuous separation of cyclic adenosine monophosphate provided by the present invention.

[0044] A continuous ion exchange system consisting of 13 anion exchange columns is adopted, including 4 in the adsorption area, 2 in the impurity washing area, 3 in the elution area, and 4 in the regeneration area. Each ion exchange column was filled with 50 g of resin (Amberlite IRA95), the diameter of the column was 4.0 cm, and the height was 30 cm. Cyclic adenosine monophosphate supernatant is put on the column, the concentration of the upper column is 7g / L, the adsorption flow rate is 2mL / min, and the adsorption capacity is 0.087g / g wet resin; the washing solution in the washing area is 0.1mol / L ammonia water, and the flow rate is 2.7mL / min, washing impurities until no impurities (use HPLC to determine that the first anion exchange column in the washing area has no impurities flowing out); the eluent is 0.02mol / L hydrochlori...

Embodiment 2

[0046] This example is used to illustrate the method for continuous separation of cyclic adenosine monophosphate provided by the present invention.

[0047] A continuous ion exchange system consisting of 13 anion exchange columns is adopted, 5 in the adsorption area, 2 in the impurity washing area, 3 in the elution area, and 3 in the regeneration area. Each ion exchange column is packed with 1.5Kg resin (Amberlite IRA95), the diameter of the column is 10 cm, and the height is 55 cm. Cyclic adenosine monophosphate supernatant is put on the column, the concentration on the column is 10g / L, the adsorption flow rate is 65mL / min, and the adsorption capacity is 0.073g / g wet resin; min, until there is no impurity (use HPLC to confirm that the first ion exchange column in the impurity washing area has no impurity flow out); (detection with HPLC determines that the first ion exchange column in the elution zone is fully eluted); in the ion exchange column located in the regeneration zo...

Embodiment 3

[0049] This example is used to illustrate the method for continuous separation of cyclic adenosine monophosphate provided by the present invention.

[0050] A continuous ion exchange system consisting of 14 anion exchange columns is adopted, 5 in the adsorption area, 3 in the washing area, 2 in the elution area, and 4 in the regeneration area. Each ion exchange column was filled with 3Kg resin (Amberlite IRA900RF Cl), the diameter of the column was 22 cm, and the height was 100 cm. Cyclic adenosine monophosphate supernatant is put on the column, the concentration of the upper column is 8g / L, the adsorption flow rate is 140mL / min, and the adsorption capacity is 0.078g / g wet resin; the washing solution in the washing area is 0.2mol / L sodium hydroxide aqueous solution, and the flow rate is 160mL / min, washing impurities until no impurities (use HPLC to confirm that the first ion exchange column in the washing area has no impurities flowing out); the eluent is 0.6mol / L hydrochloric...

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Abstract

The invention provides a method for continuously separating cyclic adenosine monophosphate. The method uses a continuous ion exchange system, and the continuous ion exchange system includes 5-80 Anion-exchange columns in series; the method includes continuously pumping the solution containing cyclic adenosine monophosphate into the anion-exchange column located in the adsorption area for adsorption, so that the adsorbed anion-exchange column passes through the impurity washing area, the elution area and the regeneration area in sequence. zone, and then return to the adsorption zone for adsorption again; the method also includes desalting, crystallizing, and drying the eluent flowing out of the elution zone to obtain cyclic adenosine monophosphate. The separation method of the present invention is simple and easy to operate, and has good effect. Not only its equipment investment and operation cost are low, but also the separation from kilogram level to ton level can be realized according to the amount of separation. All aspects have achieved excellent results, ensuring product quality.

Description

technical field [0001] The invention relates to a method for continuously separating cyclic adenosine monophosphate. Background technique [0002] Cyclic adenosine monophosphate, also known as 3′, 5′-cyclic adenosine monophosphate (3′, 5′cyclic adenosine monophosphate, referred to as cAMP), is a protein kinase activator and a derivative of nucleotides. It is an important substance with physiological activity that widely exists in the human body. It is produced by adenosine triphosphate under the catalysis of adenylyl cyclase and can regulate various functional activities of cells. As the second messenger of hormones, hormones play a role in regulating physiological functions and substance metabolism in cells, can change the function of cell membranes, and promote calcium ions in the reticulum sarcoplasm to enter muscle fibers, thereby enhancing myocardial contraction and promoting the respiratory chain Oxidase activity can improve myocardial hypoxia, relieve symptoms of cor...

Claims

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Application Information

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IPC IPC(8): C07H19/213C07H1/06B01D15/10
Inventor 应汉杰钱文斌柏建新陈晓春陈勇熊健林晓清
Owner NANJING UNIV OF TECH
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