Preparation method and product of piglet edema disease inactivated vaccine

A technology for piglet edema disease and inactivated vaccines, applied in the direction of microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve the problems of low number of cultured bacteria and high production costs, and achieve the goal of cultivating bacteria The effect of increasing the number, optimizing the production process and reducing the production cost

Inactive Publication Date: 2011-12-07
哈药集团生物疫苗有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to overcome the defects such as low number of cultured bacteria and high production cost in the existing preparation method of piglet edema disease inactivated vaccine, and provide a new preparation method of piglet edema disease inactivated vaccine , the preparation method has the advantages of rich culture medium, low production cost and high yield

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  • Preparation method and product of piglet edema disease inactivated vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Preparation of primary slant strains: Escherichia coli C83684 strains were diluted and inoculated with synthetic plate medium for 22 hours at 37°C, and 10 typical colonies were selected to inoculate synthetic slant medium, cultivated at 37°C for 24h, to obtain primary slant strains, and placed for 4 ℃ refrigerator for use;

[0029] 2. Preparation of secondary strains: Inoculate the primary slant strains into the synthetic medium and incubate in a full-temperature shaker at 37°C for 16 hours. After the pure test confirms the sterility, obtain the secondary strains for use;

[0030] 3. Bacterial liquid culture: inoculate the secondary bacteria into the synthetic medium and cultivate it in a biological fermentation tank; the culture temperature is 37°C, the rotation speed is 100r / min, and the air intake volume is 18m 3 / h, the inner pressure of the tank is 0.05Mpa, and the incubation time is 12h; after the live bacteria are counted on the sampling plate, and the sterili...

Embodiment 2

[0033] 1. Preparation of primary slant strains: Escherichia coli C83684 strains were diluted and inoculated with synthetic plate medium for 24 hours at 37°C, and 20 typical colonies were inoculated into synthetic slant medium, cultivated at 37°C for 24 hours, to obtain primary slant strains, and placed for 4 ℃ refrigerator for use;

[0034] 2. Preparation of secondary strains: Inoculate the primary slant strains into the synthetic medium and incubate in a full-temperature shaker at 37°C for 16 hours. After the pure test confirms the sterility, obtain the secondary strains for use;

[0035] 3. Bacterial liquid culture: inoculate the secondary bacteria into the synthetic medium and cultivate it in a biological fermentation tank; the culture temperature is 37°C, the speed is 200r / min, and the air intake is 18m 3 / h, the inner pressure of the tank is 0.05Mpa, and the incubation time is 12h; after the live bacteria are counted on the sampling plate, and the sterility is confirmed b...

Embodiment 3

[0038] 1. Preparation of primary slant strains: Escherichia coli C83684 strains were diluted and inoculated with synthetic plate medium for 23 hours at 37°C, and 15 typical colonies were inoculated into synthetic slant medium, cultured at 37°C for 24h to obtain primary slant strains, and placed for 4 ℃ refrigerator for use;

[0039] 2. Preparation of secondary strains: Inoculate the primary slant strains into the synthetic medium and incubate in a full-temperature shaker at 37°C for 16 hours. After the pure test confirms the sterility, obtain the secondary strains for use;

[0040] 3. Bacterial liquid culture: inoculate the secondary strains into the synthetic medium and cultivate them in a biological fermentation tank; the culture temperature is 37°C, the rotation speed is 150r / min, the inner pressure of the tank is 0.05Mpa, and the culture time is 12h; during the fermentation process, Adopt the method of gradually increasing the ventilation volume, in which 0-2h static cultu...

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Abstract

The invention discloses a preparation method of an inactivated vaccine for piglet edema disease and a product thereof, comprising: (1) inoculating Escherichia coli into a synthetic plate culture medium by dilution and culturing; selecting typical colonies to inoculate a synthetic slant culture medium and culturing, Obtain the first-level slant bacteria; (2), get the first-level slant bacteria and inoculate them in the synthetic medium for shaking culture, and obtain the second-level bacteria; (3), inoculate the second-level bacteria into the synthetic medium for fermentation and cultivation; The inactivated fermentation medium is obtained. The present invention aims at the deficiencies in the preparation method of piglet edema disease inactivated vaccines, such as low nutritional content of the culture medium, high production cost, and low yield, and improves the formula of the culture medium and optimizes the production process. The method has been greatly improved, with an average of 27.9875 billion / ml, the culture time has been shortened by 8-10 hours, and the production cost has been effectively reduced.

Description

technical field [0001] The invention relates to a preparation method of an inactivated vaccine, in particular to a preparation method of an inactivated vaccine for piglet edema disease and an inactivated vaccine product obtained by the preparation method, and belongs to the field of production of inactivated vaccine for piglet edema disease. Background technique [0002] Piglet edema disease (Edema disease, ED) pathogens belong to Enterobacteriaceae Escherichia coli species, Shiga toxin-producing Escherichia coli. Studies have proved that there are 25 O antigen serotypes related to the occurrence of piglet edema disease, mainly including O 2, O 8, O 138, O 139, O 141 and other O antigen serotypes. Most of these strains are hemolytic, among them, C83684 Belongs to a species of O 139. Clinically, the disease mainly causes eyelid edema and neurological symptoms in piglets, some piglets are accompanied by diarrhea symptoms, eyelids, stomach wall, mesentery edema can be seen in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/108C12N1/20A61P31/04C12R1/19
CPCY02A50/30
Inventor 孟福强曲海波孙德君付丽杰丁国杰张杨王彬柴华王琪郑铁鑫田路路孙建富郝建伟谢德炎
Owner 哈药集团生物疫苗有限公司
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