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A kind of method that utilizes animal proteolytic enzyme to extract fish oil from squid viscera

A technology of animal protein hydrolysis and squid viscera, which is applied in the direction of fat oil/fat production, fermentation, and fat production, can solve the problems of poor quality and low extraction rate of fish oil, and achieve the effect of low cost, clear and transparent quality, and good quality of fish oil.

Inactive Publication Date: 2011-11-30
TAIXIANG GRP TECH DEV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting fish oil from squid viscera by using animal proteolytic enzymes to obtain fish oil with high extraction rate and fish oil quality, so as to overcome the current technical problems of low extraction rate and poor quality of fish oil

Method used

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  • A kind of method that utilizes animal proteolytic enzyme to extract fish oil from squid viscera
  • A kind of method that utilizes animal proteolytic enzyme to extract fish oil from squid viscera
  • A kind of method that utilizes animal proteolytic enzyme to extract fish oil from squid viscera

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Embodiment

[0014] Fresh squid viscera were taken, crushed and homogenized with a tissue masher, and the tissue fluid was divided into packaging bags, which could be stored in a -20 °C refrigerator when not in use. Then accurately weigh a certain mass of broken viscera, add 1 mol / L NaOH solution to adjust the pH of the reaction system to 8.0; weigh animal proteolytic enzymes, and add animal proteolytic enzymes at 900 u / g based on the weight of squid viscera , continuously stirred and mixed, raised the temperature of the reaction vessel to 50 °C, inserted a stirring bar, and stirred the enzymolysis reaction for 4 h without interruption; , the centrifugation condition is 5000 r / min, and the centrifugation time is 20 min; the reaction system after centrifugation can be divided into three layers, and the fish oil in the upper layer, the enzymatic hydrolysis solution in the lower layer and the content in the middle layer are separated respectively.

[0015] Adopting gas chromatography to the f...

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Abstract

The invention belongs to the field of biotechnology. In order to overcome the technical problems of low extraction rate and poor quality of fish oil from squid viscera, the present invention provides a method for extracting fish oil from squid viscera by using animal proteolytic enzymes. The upper layer is a clear and transparent orange-red fish oil, the lower layer is an aqueous enzymolysis solution rich in low molecular weight oligopeptides and amino acids, and the middle layer is unenzymolyzed components, just absorb the upper layer fish oil. The method has mild reaction conditions, low cost and simple operation, and can be widely used in industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically refers to a method for extracting fish oil from squid viscera by using animal proteolytic enzymes. Background technique [0002] Squid is a kind of aquatic product with a large amount of catch in my country. In 2005, the annual catch of squid in China reached 500,000 to 600,000 tons. Squid is rich in nutrition, high in protein and low in fat, and is a good raw material for aquatic product processing. A large amount of squid viscera waste was generated during squid processing, accounting for about 15.92% of the total squid. The fat content in viscera is as high as 20%, which can be used as raw material for extracting fish oil. [0003] The production methods of fish oil mainly include pressing method, light alkali hydrolysis method, solvent method, water method, enzymatic method and so on. Among them, the pressing method is mainly used for by-products of fishmeal processing plants,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/02C12P21/06
Inventor 王鹏杨小克李银塔李泽瑶胡增淼
Owner TAIXIANG GRP TECH DEV
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