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Preparation method of hydrogel cell support used for promoting multiplication of retinal pigment epithelium cells

A technology of retinal pigment and epithelial cells, applied in the field of biological materials, can solve the problems of inability to attach and effectively attach, and achieve the effect of promoting adhesion and preventing aging

Inactive Publication Date: 2013-08-14
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the deficiencies in the above-mentioned prior art, the object of the present invention is to provide a method for preparing a hydrogel cell scaffold that promotes the expansion of retinal pigment epithelial cells. The hydrogel cell scaffold prepared by this preparation method overcomes the It is prone to aging, and cannot effectively attach to the retina, and the transplanted retinal pigment epithelial cells cannot attach to the fovea of ​​the retina to form a cell layer. Realizing and promoting the adhesion, spreading and proliferation of human retinal pigment epithelial cells can play a role Function to prevent its aging

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation method of the hydrogel cell scaffold that promotes retinal pigment epithelial cell expansion, the steps are as follows:

[0018] Step 1: First prepare the electrolyte polymer monomer solution, that is, mix the electrolyte polymer monomer and the solvent in a mass ratio range of 1:10 to obtain the electrolyte polymer monomer solution, the solvent is a phosphate buffer solution with a pH value of 5.7, and the electrolyte Polymers include negatively charged acrylic acid;

[0019] Step 2: Prepare a neutral polymer monomer solution, that is, mix the neutral polymer monomer and the solvent in a mass ratio range of 1:10 to obtain a neutral polymer monomer solution, and the solvent is a phosphate buffer solution with a pH value of 5.7 , the neutral macromolecular monomer includes hydroxyethyl methacrylate;

[0020] Step 3: Prepare polymer hydrogel, the prepared electrolyte polymer monomer solution and neutral polymer monomer solution are in the range of 1:0.05 ...

Embodiment 2

[0026] The preparation method of the hydrogel cell scaffold that promotes retinal pigment epithelial cell expansion, the steps are as follows:

[0027] Step 1: First prepare the electrolyte polymer monomer solution, that is, mix the electrolyte polymer monomer and the solvent in a mass ratio range of 1:100 to obtain the electrolyte polymer monomer solution, the solvent is a phosphate buffer solution with a pH value of 6.5, and the electrolyte Polymers include methacrylic acid;

[0028] Step 2: preparing a neutral polymer monomer solution, that is, mixing the neutral polymer monomer and a solvent in a mass ratio range of 1:100 to obtain a neutral polymer monomer solution, the solvent being a phosphate buffer solution of deionized water, Neutral macromolecular monomers include methyl methacrylate;

[0029] Step 3: Prepare polymer hydrogel, the prepared electrolyte polymer monomer solution and neutral polymer monomer solution are in the range of 1:0.5 according to the molar rati...

Embodiment 3

[0035] The preparation method of the hydrogel cell scaffold that promotes retinal pigment epithelial cell expansion, the steps are as follows:

[0036] Step 1: First prepare the electrolyte polymer monomer solution, that is, mix the electrolyte polymer monomer and the solvent in a mass ratio range of 1:200 to obtain the electrolyte polymer monomer solution, the solvent is a phosphate buffer solution with a pH value of 8.0, and the electrolyte High molecular monomers include sodium styrene sulfonate;

[0037] Step 2: Prepare a neutral polymer monomer solution, that is, mix the neutral polymer monomer and a solvent in a mass ratio range of 1:10 to 1:200 to obtain a neutral polymer monomer solution, and the solvent is deionized water or pH Phosphate buffer solution with a value of 5.7 to 8.0, neutral polymers including methyl methacrylate;

[0038] Step 3: Prepare polymer hydrogel, the prepared electrolyte polymer monomer solution and neutral polymer monomer solution are in the ...

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Abstract

The invention relates to a preparation method of a hydrogel cell support used for promoting multiplication of retinal pigment epithelium cells. Electrolyte macromolecule monomer solution and neutral macromolecule monomer solution are mixed to be uniform, an initiator is added, mixing to be uniform is carried out again, deoxidizing is carried out by utilizing nitrogen under the condition of dark place, and then mixture is added into a platy composite die. The prepared hydrogel cell support overcomes the defects that the traditional hydrogel cell support is easy to be aged and can not be effectively adhered on retina and transplanted retinal pigment epithelium cells can not be adhered on fovea to form a cell layer, adhesion, spreading and multiplication of the retinal pigment epithelium cells of human body can be realized and promoted, and a macromolecule hydrogel cell support with anti-ageing function can be realized.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method of a hydrogel cell support for promoting the expansion of retinal pigment epithelial cells. Background technique [0002] Age-related macular degeneration (age-related macular degeneration, AMD) has become a disease that cannot be ignored and endangers human eye health all over the world. According to the research report of the World Health Organization, AMD blindness accounts for about 8.7% of the blind people in the world. There are about 30 million AMD patients in the world, and about 500,000 people are blinded by AMD every year. The United States reported about 1.75 million AMD patients in 2004, and the prevalence of AMD was 1.47%. By 2020, it is estimated that there will be as many as 3 million AMD patients. According to a survey conducted by Zou Haidong and others in my country, the prevalence rate of AMD among people over 50 years old...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/14C12N5/071A61L27/38C08F220/06C08F2/48
Inventor 陈咏梅刘健康冯智辉刘振齐
Owner XI AN JIAOTONG UNIV
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