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Bacterial cellulose fermentation medium

A technology of bacterial cellulose, culture medium, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as unavailability, no use of coconut meal, waste, etc.

Active Publication Date: 2017-07-11
HAINAN YEGUO FOODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Coconut meal has a high fiber content, with a crude fiber content of more than 9%. It is mainly used as feed raw materials in the animal husbandry industry, especially ruminant and pig feed. Although it has played an important role in saving food resources and reducing feeding costs function, but there are still serious wastes and unusable situations
[0004] There is currently no report on the use of coconut meal as a component of bacterial cellulose fermentation medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Add 2 weight multiples of hot water at 60° C. to the coconut meal and soak for 60 minutes, then stir with a homogenizer to form a slurry. A liquid medium was prepared according to 80% by weight of the slurry and 20% by weight of sucrose, and finally the pH value was adjusted to 4.0 with glacial acetic acid.

Embodiment 2

[0016] Add 1.5 weight multiples of hot water at 70° C. to the coconut meal and soak for 45 minutes, then stir with a homogenizer to form a slurry. According to 60% by weight of slurry, 20% by weight of glucose, and 20% by weight of coconut water, a liquid medium is prepared, and finally the pH value is adjusted to 5.0 with citric acid.

Embodiment 3

[0018] Add 1 weight multiple of hot water at 80°C to the coconut meal and soak for 30 minutes, then stir with a homogenizer to make a slurry. According to 40% by weight of slurry, 15% by weight of lactose, 40% by weight of coconut water, 4% by weight of peptone, 0.2% by weight of potassium dihydrogen phosphate, 0.2% by weight of disodium hydrogen phosphate and 0.6% by weight of zinc sulfate, a liquid medium is prepared, and finally Adjust the pH to 3.5 with glacial acetic acid.

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PUM

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Abstract

The invention relates to a culture medium used for fermenting to produce bacterial cellulose. The culture medium contains slurry obtained by uniformly mixing copra meal with water, wherein the weight of the copra meal is 30-90% of the total weight of the culture medium. In the invention, waste copra meal is adopted as a culture substrate used for fermenting the bacterial cellulose to produce fungus after treatment in general, and replaces higher-cost fermentable sugars or coconut water which is adopted in conventional culture, so that cost is reduced, the application range of the copra meal is greatly expanded, the production added value of the copra meal is increased, and resources are greatly saved; and moreover, the secondary utilization of worldwide high-yield copra meal is realized, the most important problem of the source of raw materials during the production of the bacterial cellulose is solved, and the production cost of the bacterial cellulose is also greatly reduced. The copra meal contains rich nutrient substances in spite of reduced grease content, thereby being capable of providing sufficient carbon source and nitrogen source needed by fermenting the bacterial cellulose, so that the copra meal is a very good substrate for fermentation.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a culture medium for fermenting and producing bacterial cellulose and bacterial cellulose products produced by fermenting the culture medium. Background technique [0002] Bacterial Cellulose (BC) is produced by fermentation of Gluconacetobacter xylinum, etc. Through static fermentation, film-shaped bacterial cellulose products can be produced, and through dynamic fermentation, spherical bacterial cellulose products can be produced. Film-like bacterial cellulose has good water-holding properties, high crystallinity and polymerization degree, and good biocompatibility and biodegradability. It can be widely used in food, medicine, aviation, papermaking and other fields. At present, coconut water is often used as the main component of the culture medium for fermentation to produce bacterial cellulose. Although it is cheap and easy to use, it is easy to spoil and deteriorate, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08L1/02C12R1/02
Inventor 钟春燕
Owner HAINAN YEGUO FOODS
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