Method for preparing meat flavor precursor by controlling enzymolysis-mild heating oxidation of fats

A technology of heating oxidation and meat flavor, which is applied in the field of meat flavor precursors, can solve the problems of hala taste, high energy consumption, excessive oxidation, etc., and achieve mellow and full flavor, distinctive aroma and full flavor Effect

Inactive Publication Date: 2011-11-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the first technique needs to be carried out under higher temperature and anhydrous conditions, which not only has the disadvantages of high energy consumption and air drying, but also increases the randomness of fat oxidation sites caused by high temperature, and there are more oxidation by-products. It is difficult to control oxidation in a targeted manner, and it is easy to cause excessive oxidation and produce off-flavors such as hala; although the second technology overcomes some shortcomings in the first technology, the lipoxygenase used is to catalyze the free polyunsaturated fat in fat. Oxidation of fatty acids, while the content of unsaturated fatty acids in livestock and poultry fat is low, and mainly exists in the form of triglycerides, so the catalytic oxidation efficiency is low, not enough to provide rich or capable of releasing important meat flavor precursors fat oxidation products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 70g of refined chicken fat was added to 30g of 0.1mol / L pH=6 phosphate buffer (substrate concentration 70%), the mixture was placed in an enzymolysis reactor, stirred slowly, and 1200U / g was added after the chicken fat was melted The substrate lipase MER was hydrolyzed at 37°C for 2 hours, and the enzyme was inactivated to obtain the hydrolyzed product.

[0020] Add the above chicken fat enzymatic hydrolyzate into a 250mL four-necked flask equipped with an electric stirrer, a mercury thermometer, an air inlet tube and a reflux condenser, and stir the reaction with air at 60°C, the stirring speed is 100r / min, and the air flow rate is 60L / h, stop the reaction after 3h of oxidation, cool down and let stand to stratify. The oxidized chicken fat in the upper layer is the product. Its peroxide value is 81.39meq / kg fat, and its acid value is 39.68mgKOH / g fat.

Embodiment 2

[0022] Add 33g of 0.05mol / L pH=6.5 phosphate buffer solution (substrate concentration 60%) to 50g of refined lard, place the mixture in an enzymolysis reactor, stir slowly until the lard is melted, add 1000U / g bottom The lipase DF was hydrolyzed at 38°C for 3 hours to inactivate the enzyme to obtain the hydrolyzed product.

[0023] Add the above pig fat enzymatic hydrolyzate into a 250mL four-necked flask equipped with an electric stirrer, a mercury thermometer, an air inlet tube and a reflux condenser, and stir the reaction with air at 80°C, the stirring speed is 150r / min, and the air flow rate is 75L / h, stop the reaction after 3h of oxidation, cool and stand for stratification, the upper layer of oxidized lard is the product, its peroxidation value is 120.36meq / kg fat, and its acid value is 46.29mgKOH / g fat.

Embodiment 3

[0025] Add 20 g of 0.05 mol / L pH=6 phosphate buffer (substrate concentration 80%) to 80 g of refined tallow, place the mixture in an enzymolysis reactor, stir slowly until the tallow is melted, then add 2500 U / g of substrate lipase MER was hydrolyzed at 40°C for 5 hours, and the enzyme was inactivated to obtain the hydrolyzed product.

[0026] Add the above tallow enzymatic hydrolyzate into a 250mL four-neck flask equipped with an electric stirrer, a mercury thermometer, an air inlet tube and a reflux condenser, and stir the reaction with air at 100°C. The stirring speed is 200r / min, and the air flow rate is 80L / min. h, stop the reaction after 2 hours of oxidation, cool and stand for stratification, the oxidized tallow in the upper layer is the product, its peroxidation value is 111.96meq / kg fat, and its acid value is 51.24mgKOH / g fat.

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Abstract

The invention discloses a method for preparing a meat flavor precursor by firstly utilizing lipase to carry out proper enzymolysis on fats and then regulating oxidation of enzymolysis products through mild heating. The method is characterized by comprising the following steps: firstly adding 10-50g of 0.05-0.2mol / L phosphate buffer with the pH value of 6-10 into 50-100g of fats and adding 1000-2500U / g of lipase of a substrate to carry out enzymolysis at 30-50 DEG C for 2-6 hours to obtain the fat enzymolysis products; and then pumping air into the enzymolysis products to carry out heating oxidation, wherein the concentration of the substrate is 50-90%; the air flow is 5-150L / 100g.h; the oxidation temperature is 50-100 DEG C; the oxidation time is 30 minutes to 4 hours; and the stirring speed is 100-500r / min (revolutions per minute). The method has the advantages of mild oxidation condition, easiness in regulation and oxidation, less byproducts, low energy consumption and easiness in realizing industrial enlargement. The prepared fat oxidation products are rich in the characteristic meat flavor precursor, and combined with the sensory evaluation of the Maillard reaction products, the selected oxidized fats can be used for producing the meat flavor with full flavor, prominent characteristic aroma, aromatic flavor and stable quality.

Description

technical field [0001] The invention relates to a kind of raw material of animal fat, which first hydrolyzes part or all of the fat into free fatty acid under the catalysis of lipase, and then uses mild heating conditions to control its oxidation, so as to obtain the meat-flavored pre-preserved meat flavor rich in characteristic meat flavor. bodily method. Background technique [0002] The Maillard reaction has always been a hot topic in the study of meat flavor, and studies have shown that the basic meat aroma and general taste of cooked meat can be obtained by heating water-soluble components in meat (such as amino acids, peptides, carbohydrates, nucleotides, thiamine, etc.), but the characteristic flavor difference of different varieties of meat is formed by the heating of its specific lipids. Volatile derivatives produced by thermal degradation of lipids play an important role in the formation of ideal meat aroma whether they are directly used as aroma compounds or as r...

Claims

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Application Information

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IPC IPC(8): A23L1/231A23L27/26
Inventor 张晓鸣宋诗清袁霖吕怡黄梅桂史孝霞张亚婷
Owner JIANGNAN UNIV
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