Galactoside compound and preparation method thereof

A technology of galactoside and galactosidase, applied in the field of galactoside compounds and their preparation, to achieve the effect of promoting intestinal microecological balance

Active Publication Date: 2011-11-16
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, with the improvement of people's requirements for food quality, a single sucralose can no longer meet people's needs. Therefore, using sucralose as a raw material, glycosylation modification by glycosidase, and the development of new compounds have great potential in the food industry. potential application value

Method used

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  • Galactoside compound and preparation method thereof
  • Galactoside compound and preparation method thereof
  • Galactoside compound and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A novel galactosyl compound β-6-galactosyl-sucralose, the preparation steps of which are as follows:

[0030] 1. Preparation of β-galactosidase

[0031] Prepare β-galactosidase according to the method described in patent ZL2005 1 0044897.9.

[0032] 2. Synthesis of β-6-galactosyl-sucralose catalyzed by β-galactosidase

[0033] The enzyme activity of the above-mentioned β-galactosidase enzyme solution was measured, and it was diluted to 30 U / mL with pH 7.0, 50 mM potassium phosphate buffer solution. Prepare 0.6M lactose solution and 0.3M sucralose solution with pH 7.0, 50mM potassium phosphate buffer. Take 2mL diluted enzyme solution, mix 9mL lactose solution and 9mL sucralose solution, react at 45°C for 10 hours, then boil at 100°C for 10 minutes to terminate the reaction.

[0034] 3. Purification of β-6-galactosyl-sucralose

[0035] The boiled reaction solution was centrifuged at 12000 rpm for 20 minutes, and the supernatant was filtered with a 0.4 μm filter membra...

Embodiment 2

[0040] A novel galactosyl compound β-6-galactosyl-sucralose, its preparation steps compared with Example 1, the difference is:

[0041] The β-galactosidase was prepared according to the method described in the patent ZL2005 1 0044898.3 by using the gene whose GenBank accession number is CAI98003.

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Abstract

The invention relates to a galactoside compound and a preparation method thereof, and particularly relates to a method for synthesizing a galactoside compound beta-6-galactosyl-sucralose by utilizing beta-galactosidase, belonging to the technical field of sugar engineering. The galactoside compound has a chemical name of 1,6-dichloro-1,6-dideoxy-beta-D-fructofuranosyl-4-chloro-4-deoxy-alpha-D-galactopyranosyl-beta-D-galactopyranose and a molecular formula of C18H29O13C13.

Description

technical field [0001] The invention relates to a galactoside compound and a preparation method thereof, in particular to a method for synthesizing a galactoside compound β-6-galactosyl-sucralose by using β-galactosidase, and belongs to the technical field of sugar engineering. technical background [0002] Sucralose is an excellent sweetener made from sucrose. Its sweetness can reach 600 times that of sucrose, 2 times that of saccharin, and 4 times that of aspartame. In addition to extremely high sweetness, this sugar also has many advantages such as low calorie, pure sweet taste, high safety, stability and easy dissolution, and is now widely used in the food and beverage industry. [0003] For example, the Chinese patent application with publication number CN101812095A (application number 201010166831.8) discloses a method for preparing sucralose using a new acetylation reagent, which includes three steps: (1) acylation reaction, sucrose and acetylation Chemical reagent r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H5/02C12P19/14
Inventor 肖敏徐淑泽卢丽丽
Owner SHANDONG UNIV
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