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High-efficiency high-yield production method of mycorrhizal fungi

A production method, the technology of mycorrhizal fungus, applied in the field of high-efficiency incremental production of mycorrhizal fungi, can solve the problems of industrial production of VAMF mycorrhizal fungi, technical limitations of large-scale application, high disinfection technology, etc., and achieve improved seed activation energy, increase the growth rate, the effect of fast growth

Inactive Publication Date: 2011-11-02
刘方一
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are several methods for producing mycorrhizal fungi: field culture method, potted substrate culture method, static nutrient solution culture method, nutrient solution flow culture method, etc. The inoculum used in the field is mainly inoculated with soil and soilless substrates. body, whose disadvantages are not clear, the material is bulky and heavy, and the soil needs to be sterilized
For the production of tissue culture seedlings, sterile and pure propagules are used as the inoculum. The disadvantages are high disinfection technology and high cost. Currently, it is limited to research
[0006] The pure culture technology of VAMF mycorrhizal fungus in my country has not yet been solved, and the commercialization and factory production of VAMF mycorrhizal fungus cannot be carried out, and the large-scale application technology is still limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A microbial nutrient solution for the cultivation of mycorrhizal fungi, which is fermented from the following raw materials in weight percentage: 5% rice bran, 10% soybean powder, 3% bone meal, 4% brown sugar, Su Kehan ​​(Weifang) Bioengineering Co., Ltd. The company produces 0.5% prebiotics and 77.5% micro-nano bubble water.

[0044]The microbial nutrient solution is prepared by fermentation at a temperature of 20-30° C., stirring twice a day for 25 days.

Embodiment 2

[0046] A method for incremental production of mycorrhizal bacteria, comprising the following steps:

[0047] a) Select Bahia grass as the host plant, sow the Bahia grass seeds on a seedbed equipped with simple perlite, pour it thoroughly with micro-nano bubble water, and water it once a day with the microbial nutrient solution obtained in Example 1 diluted 200 times , cultivated for 14 days in total;

[0048] b) Take out the Bahia Grass cultivated in step a), inoculate its roots with a mixture of mycorrhizal fungi and perlite containing >120 spores, then plant them on the seedbed, and use Example 1 diluted 200 times every day to obtain The microbial nutrient solution was watered once and grown for 14 days;

[0049] c) Wrap the bahia grass stem obtained in step b) with a sponge, place it in a planar cultivation device, provide the root system with the microbial nutrient solution by spraying, and keep the root humidity above 95%. The microbial nutrient solution is diluted 100 ...

Embodiment 3

[0052] A method for incremental production of mycorrhizal bacteria, comprising the following steps:

[0053] a) Select Bahia grass as the host plant, first treat the Bahia grass seeds in an electrostatic field for 5 minutes, the input voltage of the electrostatic field is 220V, the output voltage is 0-100 kV, and the output current is 1 mA, and then Sow on the seedbed that is the mixture of perlite and vermiculite that the weight ratio of 3:1 is housed, pour thoroughly with micro-nano bubble water, water once with the microbial nutrient solution that the embodiment 1 that dilutes 200 times obtains every day, cultivate 14 in total sky;

[0054] b) Take out the Bahia Grass cultivated in step a), inoculate its roots with a mixture of mycorrhizal fungi and perlite containing >120 spores, then plant them on the seedbed, and use Example 1 diluted 200 times every day to obtain The microbial nutrient solution was watered once and grown for 14 days;

[0055] c) Wrap the bahia grass s...

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Abstract

The invention provides a high-efficiency high-yield production method of mycorrhizal fungi, integrates multiple technical characteristics of biological nutrient fluids, tower-shaped plate-type three-dimensional cultivation racks and electrostatic field treatment of host plant seeds, and achieves the effect of high-efficiency high-yield production of mycorrhizal fungi. In the whole production process, the utilization of tower-shaped plates maximizes the use of space and increases the planting area. Considering the nutrient fluid preparation, instead of the conventional inorganic nutrient fluids, organic nutrient fluids which are prepared by fermenting formulated ingredients with microorganisms are adopted and sprayed to the root systems of plants, and the residual fluids are returned to a nutrient fluid tank to achieve the recycling of the nutrient fluids. The purpose of zero discharge is achieved by the symbiosis of microorganisms and plants.

Description

technical field [0001] The invention is a method for producing fungi, in particular to a method for producing mycorrhizal fungi. Background technique [0002] With the increase of industrial wastewater, the unreasonable use of chemical fertilizers and pesticides, and the excess of heavy metals, soil compaction, aggravation of salinization, lack of trace elements, serious imbalance of beneficial bacteria in the soil, etc., the deterioration of the soil is constantly endangering human health. . And microbial fertilizer is being paid more and more attention by people, as the key of soil improvement. [0003] Mycorrhizal fungi are vesicular-arbuscular mycorrhizal fungi (VAMF) for short. In the symbiosis between higher plants and mycorrhizal fungi, carbohydrates produced by photosynthesis are shared by host plants and fungi. Some of the nutrients that fungi absorb from the soil are also passed on to plants, and the fungi and plant roots jointly absorb and synthesize nutrients f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 刘方一
Owner 刘方一
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